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Q6IM78 (TOP1M_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase I, mitochondrial

Short name=TOP1mt
EC=5.99.1.2
Gene names
Name:Top1mt
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length593 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Cofactor

Divalent metal ions (calcium or magnesium) By similarity.

Subcellular location

Mitochondrion By similarity.

Sequence similarities

Belongs to the type IB topoisomerase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4343Mitochondrion By similarity
Chain44 – 593550DNA topoisomerase I, mitochondrial
PRO_0000384395

Regions

Region254 – 2552Interaction with DNA By similarity
Region317 – 3226Interaction with DNA By similarity
Region414 – 4163Interaction with DNA By similarity

Sites

Active site5511O-(3'-phospho-DNA)-tyrosine intermediate By similarity
Site1451Interaction with DNA By similarity
Site1931Interaction with DNA By similarity
Site2411Interaction with DNA By similarity
Site2721Interaction with DNA By similarity
Site3301Interaction with DNA By similarity
Site3611Interaction with DNA By similarity
Site4031Interaction with DNA By similarity
Site4611Interaction with DNA By similarity
Site4791Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6IM78 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 6B691D668970BD9E

FASTA59369,011
        10         20         30         40         50         60 
MLLLWLRALC RRFQHVPRRV PSRQVSRGSK ASRAGWGETS KSSVKWKQLE HKGPCFAPAY 

        70         80         90        100        110        120 
EPLPDGVRFF YDGKPVRLSL AAEEVATFYG KMLHLECTTK EVFRRNFFSD WQKEMTAEER 

       130        140        150        160        170        180 
KLITHLDKCD FSEIHRHFME RAEARRTLPR EQKQKLKEEA EKLQQEFGYC ILDGHREKIG 

       190        200        210        220        230        240 
NFKTEPPGLF RGRGDHPKMG MLKRRVMPED VVINCSRDSK IPEPPAGHQW KEVRSDNTVM 

       250        260        270        280        290        300 
WLAAWVENIQ NSFKYIILNP SSKPKGEMDW QKYEVARRLK GVVDKIRAQY QADWKSPEMK 

       310        320        330        340        350        360 
KRQLAVALYF IDKLALRTGN EKEEGETADT VGCCSLRVEH VRLHTPADGQ EHVVELDFLG 

       370        380        390        400        410        420 
KDSIRYKNHV TVEKLVFQNL QHFMEDKDPR DDLFDALTTS SLNKHLQDLM EGLTAKVFRT 

       430        440        450        460        470        480 
YNASITLQEQ LRVLTRAEDS LTCKVLAYNR ANRAVAVLCN HQRAIPKTFE ESMQTLQKKI 

       490        500        510        520        530        540 
ETKKAQVAEA QVELQKAETD LRMRGDSKSK SFLQKQQRLL KLEEQLARLC TKATDKEENK 

       550        560        570        580        590 
QVALGTAKLN YLDPRISIAW CKRFGVPVEK IYNKTQRERF AWAFNQAGED FEF 

« Hide

References

[1]"Thirteen-exon-motif signature for vertebrate nuclear and mitochondrial type IB topoisomerases."
Zhang H., Meng L.-H., Zimonjic D.B., Popescu N.C., Pommier Y.
Nucleic Acids Res. 32:2087-2092(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BK001786 mRNA. Translation: DAA02296.1.
RefSeqNP_001002798.1. NM_001002798.1.
UniGeneRn.31307.

3D structure databases

ProteinModelPortalQ6IM78.
SMRQ6IM78. Positions 38-593.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000009965.

Proteomic databases

PaxDbQ6IM78.
PRIDEQ6IM78.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000009965; ENSRNOP00000009965; ENSRNOG00000007500.
GeneID300029.
KEGGrno:300029.
UCSCRGD:1303177. rat.

Organism-specific databases

CTD116447.
RGD1303177. Top1mt.

Phylogenomic databases

eggNOGCOG3569.
GeneTreeENSGT00390000016347.
HOGENOMHOG000105469.
InParanoidQ6IM78.
KOK03163.
OMAVINCSRD.
OrthoDBEOG7CVPX5.
PhylomeDBQ6IM78.
TreeFamTF105281.

Gene expression databases

GenevestigatorQ6IM78.

Family and domain databases

Gene3D1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SMARTSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
ProtoNetSearch...

Other

NextBio646149.
PROQ6IM78.

Entry information

Entry nameTOP1M_RAT
AccessionPrimary (citable) accession number: Q6IM78
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families