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Protein

DNA topoisomerase I, mitochondrial

Gene

Top1mt

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Cofactori

Ca2+By similarity, Mg2+By similarityNote: Divalent metal ions (calcium or magnesium).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei551O-(3'-phospho-DNA)-tyrosine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase I, mitochondrial (EC:5.99.1.2)
Short name:
TOP1mt
Gene namesi
Name:Top1mt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1303177 Top1mt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43MitochondrionBy similarityAdd BLAST43
ChainiPRO_000038439544 – 593DNA topoisomerase I, mitochondrialAdd BLAST550

Proteomic databases

PaxDbiQ6IM78
PRIDEiQ6IM78

Expressioni

Gene expression databases

BgeeiENSRNOG00000007500
ExpressionAtlasiQ6IM78 baseline and differential
GenevisibleiQ6IM78 RN

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei145Interaction with DNABy similarity1
Sitei193Interaction with DNABy similarity1
Sitei241Interaction with DNABy similarity1
Sitei272Interaction with DNABy similarity1
Sitei330Interaction with DNABy similarity1
Sitei361Interaction with DNABy similarity1
Sitei403Interaction with DNABy similarity1
Sitei461Interaction with DNABy similarity1
Sitei479Interaction with DNABy similarity1

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009965

Structurei

3D structure databases

ProteinModelPortaliQ6IM78
SMRiQ6IM78
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 255Interaction with DNABy similarity2
Regioni317 – 322Interaction with DNABy similarity6
Regioni414 – 416Interaction with DNABy similarity3

Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0981 Eukaryota
COG3569 LUCA
GeneTreeiENSGT00390000016347
HOGENOMiHOG000105469
InParanoidiQ6IM78
KOiK03163
OMAiVINCSRD
OrthoDBiEOG091G02KT
PhylomeDBiQ6IM78
TreeFamiTF105281

Family and domain databases

CDDicd00659 Topo_IB_C, 1 hit
Gene3Di1.10.10.41, 1 hit
1.10.132.10, 1 hit
2.170.11.10, 2 hits
3.90.15.10, 1 hit
InterProiView protein in InterPro
IPR011010 DNA_brk_join_enz
IPR013034 DNA_topo_DNA_db_N_dom1
IPR013030 DNA_topo_DNA_db_N_dom2
IPR001631 TopoI
IPR025834 TopoI_C_dom
IPR014711 TopoI_cat_a-hlx-sub_euk
IPR014727 TopoI_cat_a/b-sub_euk
IPR013500 TopoI_cat_euk
IPR008336 TopoI_DNA-bd_euk
IPR036202 TopoI_DNA-bd_euk_N_sf
IPR013499 TopoI_euk
PANTHERiPTHR10290 PTHR10290, 1 hit
PfamiView protein in Pfam
PF14370 Topo_C_assoc, 1 hit
PF01028 Topoisom_I, 1 hit
PF02919 Topoisom_I_N, 1 hit
PRINTSiPR00416 EUTPISMRASEI
SMARTiView protein in SMART
SM00435 TOPEUc, 1 hit
SUPFAMiSSF56349 SSF56349, 2 hits
SSF56741 SSF56741, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6IM78-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLWLRALC RRFQHVPRRV PSRQVSRGSK ASRAGWGETS KSSVKWKQLE
60 70 80 90 100
HKGPCFAPAY EPLPDGVRFF YDGKPVRLSL AAEEVATFYG KMLHLECTTK
110 120 130 140 150
EVFRRNFFSD WQKEMTAEER KLITHLDKCD FSEIHRHFME RAEARRTLPR
160 170 180 190 200
EQKQKLKEEA EKLQQEFGYC ILDGHREKIG NFKTEPPGLF RGRGDHPKMG
210 220 230 240 250
MLKRRVMPED VVINCSRDSK IPEPPAGHQW KEVRSDNTVM WLAAWVENIQ
260 270 280 290 300
NSFKYIILNP SSKPKGEMDW QKYEVARRLK GVVDKIRAQY QADWKSPEMK
310 320 330 340 350
KRQLAVALYF IDKLALRTGN EKEEGETADT VGCCSLRVEH VRLHTPADGQ
360 370 380 390 400
EHVVELDFLG KDSIRYKNHV TVEKLVFQNL QHFMEDKDPR DDLFDALTTS
410 420 430 440 450
SLNKHLQDLM EGLTAKVFRT YNASITLQEQ LRVLTRAEDS LTCKVLAYNR
460 470 480 490 500
ANRAVAVLCN HQRAIPKTFE ESMQTLQKKI ETKKAQVAEA QVELQKAETD
510 520 530 540 550
LRMRGDSKSK SFLQKQQRLL KLEEQLARLC TKATDKEENK QVALGTAKLN
560 570 580 590
YLDPRISIAW CKRFGVPVEK IYNKTQRERF AWAFNQAGED FEF
Length:593
Mass (Da):69,011
Last modified:July 5, 2004 - v1
Checksum:i6B691D668970BD9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BK001786 mRNA Translation: DAA02296.1
RefSeqiNP_001002798.1, NM_001002798.1
UniGeneiRn.31307

Genome annotation databases

EnsembliENSRNOT00000009965; ENSRNOP00000009965; ENSRNOG00000007500
GeneIDi300029
KEGGirno:300029
UCSCiRGD:1303177 rat

Similar proteinsi

Entry informationi

Entry nameiTOP1M_RAT
AccessioniPrimary (citable) accession number: Q6IM78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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