Reviewed, UniProtKB/Swiss-Prot Q6IE81 (JADE1_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein Jade-1
Alternative name(s):
    PHD finger protein 17

Gene names

Name:	PHF17
Synonyms:	JADE1, KIAA1807

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	842 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator it may also promote acetylation of nucleosomal histone H4 by HTATIP. Promotes apoptosis. May act as a renal tumor suppressor.
Subunit structure	Component of the HBO1 complex composed at least of ING4 or ING5, MYTS2/HBO1, EAF6, and one of PHF15, PHF16 and PHF17. Isoform 3 interacts with VHL and HTATIP.
Subcellular location	Cytoplasm. Nucleus. Ref.7 Ref.1
Tissue specificity	Highly expressed in kidney. Also present in pancreas, liver and heart (at protein level). Down-regulated in renal cancer cells. Ref.8 Ref.1
Domain	The 2 PHD-type zinc fingers are required for transcriptional activity.
Sequence similarities	Belongs to the JADE family. Contains 2 PHD-type zinc fingers.

Ontologies

Keywords
Biological process	Apoptosis Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	apoptosis Ref.1 Non-traceable author statement. Source: UniProtKB negative regulation of cell growth Ref.1 Non-traceable author statement. Source: UniProtKB regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to stress Ref.1 Non-traceable author statement. Source: UniProtKB transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: UniProtKB nucleus Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6IE81-1)

Also known as: JADE1L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6IE81-2)

The sequence of this isoform differs from the canonical sequence as follows:
     99-110: Missing.

Note: No experimental confirmation available.

Isoform 3 (identifier: Q6IE81-3)

Also known as: JADE1S;

The sequence of this isoform differs from the canonical sequence as follows:
     503-509: RNLTYMV → MIDTDTL
     510-842: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 842	842	Protein Jade-1		PRO_0000253529
Regions
	Zinc finger	203 – 253	51	PHD-type 1
	Zinc finger	312 – 371	60	PHD-type 2; atypical
	Compositional bias	544 – 550	7	Poly-Ser
Amino acid modifications
	Modified residue	8	1	Phosphoserine Ref.9
	Modified residue	9	1	Phosphoserine Ref.9
	Modified residue	10	1	Phosphoserine Ref.9
	Modified residue	13	1	Phosphoserine Ref.9
	Modified residue	89	1	Phosphoserine Ref.11
	Modified residue	92	1	Phosphothreonine Ref.11
Natural variations
	Alternative sequence	99 – 110	12	Missing in isoform 2.		VSP_021045
	Alternative sequence	503 – 509	7	RNLTYMV → MIDTDTL in isoform 3.		VSP_021046
	Alternative sequence	510 – 842	333	Missing in isoform 3.		VSP_021047
	Natural variant	662	1	N → S: dbSNP rs6855813.		VAR_053777

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (JADE1L) [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 239FC2229CE25FFD	FASTA	842	95,533
	10 20 30 40 50 60 MKRGRLPSSS EDSDDNGSLS TTWSQNSRSQ HRRSSCSRHE DRKPSEVFRT DLITAMKLHD 70 80 90 100 110 120 SYQLNPDEYY VLADPWRQEW EKGVQVPVSP GTIPQPVARV VSEEKSLMFI RPKKYIVSSG 130 140 150 160 170 180 SEPPELGYVD IRTLADSVCR YDLNDMDAAW LELTNEEFKE MGMPELDEYT MERVLEEFEQ 190 200 210 220 230 240 RCYDNMNHAI ETEEGLGIEY DEDVVCDVCQ SPDGEDGNEM VFCDKCNICV HQACYGILKV 250 260 270 280 290 300 PEGSWLCRTC ALGVQPKCLL CPKKGGAMKP TRSGTKWVHV SCALWIPEVS IGSPEKMEPI 310 320 330 340 350 360 TKVSHIPSSR WALVCSLCNE KFGASIQCSV KNCRTAFHVT CAFDRGLEMK TILAENDEVK 370 380 390 400 410 420 FKSYCPKHSS HRKPEESLGK GAAQENGAPE CSPRNPLEPF ASLEQNREEA HRVSVRKQKL 430 440 450 460 470 480 QQLEDEFYTF VNLLDVARAL RLPEEVVDFL YQYWKLKRKV NFNKPLITPK KDEEDNLAKR 490 500 510 520 530 540 EQDVLFRRLQ LFTHLRQDLE RVRNLTYMVT RREKIKRSVC KVQEQIFNLY TKLLEQERVS 550 560 570 580 590 600 GVPSSCSSSS LENMLLFNSP SVGPDAPKIE DLKWHSAFFR KQMGTSLVHS LKKPHKRDPL 610 620 630 640 650 660 QNSPGSEGKT LLKQPDLCGR REGMVVPESF LGLEKTFAEA RLISAQQKNG VVMPDHGKRR 670 680 690 700 710 720 DNRFHCDLIK GDLKDKSFKQ SHKPLRSTDV SQRHLDNTRA ATSPGVGQSA PGTRKEIVPK 730 740 750 760 770 780 CNGSLIKVNY NQTAVKVPTT PASPVKNWGG FRIPKKGERQ QQGEAHDGAC HQHSDYPYLG 790 800 810 820 830 840 LGRVPAKERA KSKLKSDNEN DGYVPDVEMS DSESEASEKK CIHTSSTISR RTDIIRRSIL AS
	Isoform 2. Checksum: 3F136EE584113837	FASTA	830	94,114
	10 20 30 40 50 60 MKRGRLPSSS EDSDDNGSLS TTWSQNSRSQ HRRSSCSRHE DRKPSEVFRT DLITAMKLHD 70 80 90 100 110 120 SYQLNPDEYY VLADPWRQEW EKGVQVPVSP GTIPQPVARP KKYIVSSGSE PPELGYVDIR 130 140 150 160 170 180 TLADSVCRYD LNDMDAAWLE LTNEEFKEMG MPELDEYTME RVLEEFEQRC YDNMNHAIET 190 200 210 220 230 240 EEGLGIEYDE DVVCDVCQSP DGEDGNEMVF CDKCNICVHQ ACYGILKVPE GSWLCRTCAL 250 260 270 280 290 300 GVQPKCLLCP KKGGAMKPTR SGTKWVHVSC ALWIPEVSIG SPEKMEPITK VSHIPSSRWA 310 320 330 340 350 360 LVCSLCNEKF GASIQCSVKN CRTAFHVTCA FDRGLEMKTI LAENDEVKFK SYCPKHSSHR 370 380 390 400 410 420 KPEESLGKGA AQENGAPECS PRNPLEPFAS LEQNREEAHR VSVRKQKLQQ LEDEFYTFVN 430 440 450 460 470 480 LLDVARALRL PEEVVDFLYQ YWKLKRKVNF NKPLITPKKD EEDNLAKREQ DVLFRRLQLF 490 500 510 520 530 540 THLRQDLERV RNLTYMVTRR EKIKRSVCKV QEQIFNLYTK LLEQERVSGV PSSCSSSSLE 550 560 570 580 590 600 NMLLFNSPSV GPDAPKIEDL KWHSAFFRKQ MGTSLVHSLK KPHKRDPLQN SPGSEGKTLL 610 620 630 640 650 660 KQPDLCGRRE GMVVPESFLG LEKTFAEARL ISAQQKNGVV MPDHGKRRDN RFHCDLIKGD 670 680 690 700 710 720 LKDKSFKQSH KPLRSTDVSQ RHLDNTRAAT SPGVGQSAPG TRKEIVPKCN GSLIKVNYNQ 730 740 750 760 770 780 TAVKVPTTPA SPVKNWGGFR IPKKGERQQQ GEAHDGACHQ HSDYPYLGLG RVPAKERAKS 790 800 810 820 830 KLKSDNENDG YVPDVEMSDS ESEASEKKCI HTSSTISRRT DIIRRSILAS
	Isoform 3 (JADE1S). Checksum: ED47BFCFAE89A24F	FASTA	509	58,370
	10 20 30 40 50 60 MKRGRLPSSS EDSDDNGSLS TTWSQNSRSQ HRRSSCSRHE DRKPSEVFRT DLITAMKLHD 70 80 90 100 110 120 SYQLNPDEYY VLADPWRQEW EKGVQVPVSP GTIPQPVARV VSEEKSLMFI RPKKYIVSSG 130 140 150 160 170 180 SEPPELGYVD IRTLADSVCR YDLNDMDAAW LELTNEEFKE MGMPELDEYT MERVLEEFEQ 190 200 210 220 230 240 RCYDNMNHAI ETEEGLGIEY DEDVVCDVCQ SPDGEDGNEM VFCDKCNICV HQACYGILKV 250 260 270 280 290 300 PEGSWLCRTC ALGVQPKCLL CPKKGGAMKP TRSGTKWVHV SCALWIPEVS IGSPEKMEPI 310 320 330 340 350 360 TKVSHIPSSR WALVCSLCNE KFGASIQCSV KNCRTAFHVT CAFDRGLEMK TILAENDEVK 370 380 390 400 410 420 FKSYCPKHSS HRKPEESLGK GAAQENGAPE CSPRNPLEPF ASLEQNREEA HRVSVRKQKL 430 440 450 460 470 480 QQLEDEFYTF VNLLDVARAL RLPEEVVDFL YQYWKLKRKV NFNKPLITPK KDEEDNLAKR 490 500 EQDVLFRRLQ LFTHLRQDLE RVMIDTDTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The von Hippel-Lindau tumor suppressor stabilizes novel plant homeodomain protein Jade-1." Zhou M.I., Wang H., Ross J.J., Kuzmin I., Xu C., Cohen H.T. J. Biol. Chem. 277:39887-39898(2002) [PubMed: 12169691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), INTERACTION WITH VHL, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3). Tissue: Hippocampus.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Tissue: Cervix.
[5]	"Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O. DNA Res. 8:85-95(2001) [PubMed: 11347906] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 141-842. Tissue: Brain.
[6]	"Identification of Jade1, a gene encoding a PHD zinc finger protein, in a gene trap mutagenesis screen for genes involved in anteroposterior axis development." Tzouanacou E., Tweedie S., Wilson V. Mol. Cell. Biol. 23:8553-8562(2003) [PubMed: 14612400] [Abstract] Cited for: IDENTIFICATION (ISOFORM 1).
[7]	"von Hippel-Lindau partner Jade-1 is a transcriptional co-activator associated with histone acetyltransferase activity." Panchenko M.V., Zhou M.I., Cohen H.T. J. Biol. Chem. 279:56032-56041(2004) [PubMed: 15502158] [Abstract] Cited for: INTERACTION WITH HTATIP, SUBCELLULAR LOCATION, FUNCTION.
[8]	"Jade-1, a candidate renal tumor suppressor that promotes apoptosis." Zhou M.I., Foy R.L., Chitalia V.C., Zhao J., Panchenko M.V., Wang H., Cohen H.T. Proc. Natl. Acad. Sci. U.S.A. 102:11035-11040(2005) [PubMed: 16046545] [Abstract] Cited for: TISSUE SPECIFICITY, FUNCTION.
[9]	"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M. Cell 127:635-648(2006) [PubMed: 17081983] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8; SER-9; SER-10 AND SER-13, MASS SPECTROMETRY. Tissue: Epithelium.
[10]	"ING tumor suppressor proteins are critical regulators of chromatin acetylation required for genome expression and perpetuation." Doyon Y., Cayrou C., Ullah M., Landry A.-J., Cote V., Selleck W., Lane W.S., Tan S., Yang X.-J., Cote J. Mol. Cell 21:51-64(2006) [PubMed: 16387653] [Abstract] Cited for: FUNCTION IN HISTONE H4 ACETYLATION, IDENTIFICATION BY MASS SPECTROMETRY, IDENTIFICATION IN THE HBO1 COMPLEX.
[11]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed: 18220336] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-89 AND THR-92, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF520952 mRNA. Translation: AAM95612.1. AK027620 mRNA. Translation: BAB55239.1. AK026132 mRNA. Translation: BAB15371.1. Different initiation. AK074986 mRNA. Translation: BAC11335.1. Different initiation. AK127326 mRNA. Translation: BAC86931.1. AC108024 Genomic DNA. Translation: AAY40997.1. AC093783 Genomic DNA. No translation available. BC032376 mRNA. Translation: AAH32376.1. AB058710 mRNA. Translation: BAB47436.1. BN000287 mRNA. Translation: CAE30500.1.
IPI	IPI00395424. IPI00399252. IPI00789745.
RefSeq	NP_079176.2. NP_955352.1.
UniGene	Hs.12420
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6IE81. 1 interaction.
PTM databases
PhosphoSite	Q6IE81.
Proteomic databases
PRIDE	Q6IE81.
Genome annotation databases
Ensembl	ENSG00000077684. Homo sapiens. [Contig view]
GeneID	79960.
KEGG	hsa:79960.
Organism-specific databases
GeneCards	GC04P129950.
H-InvDB	HIX0004498.
HGNC	HGNC:30027. PHF17.
MIM	610514. gene.
PharmGKB	PA134925124.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q6IE81.
OMA	Q6IE81. TPDHGKR.
Gene expression databases
ArrayExpress	Q6IE81.
Bgee	Q6IE81.
CleanEx	HS_PHF17.
GermOnline	ENSG00000077684. Homo sapiens.
Family and domain databases
InterPro	IPR019542. Enhancer_of_polycomb-like. IPR019786. Zinc_finger_PHD-type_CS. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. [Graphical view]
Pfam	PF10513. EPL1. 1 hit. PF00628. PHD. 2 hits. [Graphical view]
SMART	SM00249. PHD. 2 hits. [Graphical view]
PROSITE	PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	69955.
SOURCE	Search...

Entry information

Entry name

JADE1_HUMAN

Accession

Primary (citable) accession number: Q6IE81
Secondary accession number(s): Q4W5D5 , Q6ZSL7, Q8NC41, Q96JL8, Q96SQ1, Q9H692

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)