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Q6IE77

- NEIL2_BOVIN

UniProt

Q6IE77 - NEIL2_BOVIN

Protein

Endonuclease 8-like 2

Gene

NEIL2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity.By similarity

    Catalytic activityi

    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

    Enzyme regulationi

    Acetylation of Lys-50 leads to loss of DNA nicking activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Schiff-base intermediate with DNAPROSITE-ProRule annotation
    Active sitei3 – 31Proton donorPROSITE-ProRule annotation
    Active sitei50 – 501Proton donor; for beta-elimination activityPROSITE-ProRule annotation
    Binding sitei227 – 2271DNABy similarity
    Active sitei306 – 3061Proton donor; for delta-elimination activityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri280 – 31637FPG-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
    3. hydrolase activity, hydrolyzing N-glycosyl compounds Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. base-excision repair Source: InterPro
    2. nucleotide-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease 8-like 2 (EC:3.2.2.-, EC:4.2.99.18)
    Alternative name(s):
    DNA glycosylase/AP lyase Neil2
    DNA-(apurinic or apyrimidinic site) lyase Neil2
    Endonuclease VIII-like 2
    Nei homolog 2
    Short name:
    NEH2
    Nei-like protein 2
    Gene namesi
    Name:NEIL2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 8

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: UniProtKB-SubCell
    3. spindle microtubule Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 329328Endonuclease 8-like 2PRO_0000170907Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei50 – 501N6-acetyllysineBy similarity
    Modified residuei149 – 1491N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiQ6IE77.

    Interactioni

    Subunit structurei

    Binds EP300.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000006262.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6IE77.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The zinc-finger domain is important for DNA binding.

    Sequence similaritiesi

    Belongs to the FPG family.PROSITE-ProRule annotation
    Contains 1 FPG-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri280 – 31637FPG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG73546.
    GeneTreeiENSGT00730000110955.
    HOGENOMiHOG000069913.
    HOVERGENiHBG082014.
    InParanoidiQ6IE77.
    KOiK10568.
    OMAiSVRKFHH.
    OrthoDBiEOG7Z69CR.
    TreeFamiTF331502.

    Family and domain databases

    InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    [Graphical view]
    PfamiPF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    [Graphical view]
    SUPFAMiSSF46946. SSF46946. 1 hit.
    PROSITEiPS51068. FPG_CAT. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6IE77-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPEGPSVRKF HHLVSPFVGQ QVVKTGGSSK KLNPTSFQSL WLQDSQVHGK    50
    KLFLRFDPDE EAVSLGNSLL SEPLREGEQK DKARHHQEAS DPSSWSPGGD 100
    SAVPSGDDGL QCLGGDTPAG GAERWLQVSF GLFGSIRVNE FSRAKKANKR 150
    GDWRDPVPRL VLHFSGSGFL AFYNCQMTWR FSSPVVSPAS DILSEKFHRG 200
    QALEALGREQ PICYTLLDQR YFSGLGNIIK NEALFRAGIH PLSPGSLLGL 250
    PRLEALVDHV VAFSADWLQG KFQGTRQHTQ IYQKEQCPAG HQVVRESLGP 300
    PGGFQRLTWW CPQCQPRLSA DEPKQLQPS 329
    Length:329
    Mass (Da):36,392
    Last modified:January 23, 2007 - v3
    Checksum:i0D48CC7BEDA526EA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC107065 Genomic DNA. No translation available.
    BN000316 mRNA. Translation: CAE48362.1.
    RefSeqiNP_001013021.1. NM_001013003.1.
    XP_005209884.1. XM_005209827.1.
    UniGeneiBt.26303.

    Genome annotation databases

    EnsembliENSBTAT00000006262; ENSBTAP00000006262; ENSBTAG00000004769.
    GeneIDi444987.
    KEGGibta:444987.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC107065 Genomic DNA. No translation available.
    BN000316 mRNA. Translation: CAE48362.1 .
    RefSeqi NP_001013021.1. NM_001013003.1.
    XP_005209884.1. XM_005209827.1.
    UniGenei Bt.26303.

    3D structure databases

    ProteinModelPortali Q6IE77.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000006262.

    Proteomic databases

    PRIDEi Q6IE77.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000006262 ; ENSBTAP00000006262 ; ENSBTAG00000004769 .
    GeneIDi 444987.
    KEGGi bta:444987.

    Organism-specific databases

    CTDi 252969.

    Phylogenomic databases

    eggNOGi NOG73546.
    GeneTreei ENSGT00730000110955.
    HOGENOMi HOG000069913.
    HOVERGENi HBG082014.
    InParanoidi Q6IE77.
    KOi K10568.
    OMAi SVRKFHH.
    OrthoDBi EOG7Z69CR.
    TreeFami TF331502.

    Miscellaneous databases

    NextBioi 20831770.

    Family and domain databases

    InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    [Graphical view ]
    Pfami PF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46946. SSF46946. 1 hit.
    PROSITEi PS51068. FPG_CAT. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of taurine cattle: a window to ruminant biology and evolution."
      The bovine genome sequencing and analysis consortium
      Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hereford.
    2. "Mapping of the GATA4, NEIL2, FDFT1 genes and CTSB-associated microsatellites to the centromeric region of BTA8."
      Plis-Finarov A., Hudson H., Roe B., Ron M., Seroussi E.
      Anim. Genet. 35:154-155(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiNEIL2_BOVIN
    AccessioniPrimary (citable) accession number: Q6IE77
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 7, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 74 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3