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Protein

Endonuclease 8-like 2

Gene

NEIL2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).By similarity

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

Enzyme regulationi

Acetylation of Lys-50 leads to loss of DNA nicking activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNAPROSITE-ProRule annotation1
Active sitei3Proton donorPROSITE-ProRule annotation1
Active sitei50Proton donor; for beta-elimination activityPROSITE-ProRule annotation1
Binding sitei227DNABy similarity1
Active sitei306Proton donor; for delta-elimination activityPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri280 – 316FPG-typePROSITE-ProRule annotationAdd BLAST37

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Glycosidase, Hydrolase, Lyase, Multifunctional enzyme
Biological processDNA damage, DNA repair
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-BTA-110329 Cleavage of the damaged pyrimidine
R-BTA-5649702 APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8-like 2 (EC:3.2.2.-, EC:4.2.99.18)
Alternative name(s):
DNA glycosylase/AP lyase Neil2
DNA-(apurinic or apyrimidinic site) lyase Neil2
Endonuclease VIII-like 2
Nei homolog 2
Short name:
NEH2
Nei-like protein 2
Gene namesi
Name:NEIL2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 8

Organism-specific databases

VGNCiVGNC:31989 NEIL2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001709072 – 329Endonuclease 8-like 2Add BLAST328

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50N6-acetyllysineBy similarity1
Modified residuei68PhosphoserineBy similarity1
Modified residuei149N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ6IE77
PRIDEiQ6IE77

Expressioni

Gene expression databases

BgeeiENSBTAG00000004769

Interactioni

Subunit structurei

Binds EP300.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006262

Structurei

3D structure databases

ProteinModelPortaliQ6IE77
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The zinc-finger domain is important for DNA binding.

Sequence similaritiesi

Belongs to the FPG family.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri280 – 316FPG-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IJ89 Eukaryota
ENOG4112BNS LUCA
GeneTreeiENSGT00730000110955
HOGENOMiHOG000069913
HOVERGENiHBG082014
InParanoidiQ6IE77
KOiK10568
OMAiSPFVGQQ
OrthoDBiEOG091G0G2K
TreeFamiTF331502

Family and domain databases

InterProiView protein in InterPro
IPR015886 DNA_glyclase/AP_lyase_DNA-bd
IPR012319 FPG_cat
IPR010979 Ribosomal_S13-like_H2TH
IPR000214 Znf_DNA_glyclase/AP_lyase
PfamiView protein in Pfam
PF06831 H2TH, 1 hit
SMARTiView protein in SMART
SM01232 H2TH, 1 hit
SUPFAMiSSF46946 SSF46946, 1 hit
PROSITEiView protein in PROSITE
PS51068 FPG_CAT, 1 hit
PS51066 ZF_FPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6IE77-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEGPSVRKF HHLVSPFVGQ QVVKTGGSSK KLNPTSFQSL WLQDSQVHGK
60 70 80 90 100
KLFLRFDPDE EAVSLGNSLL SEPLREGEQK DKARHHQEAS DPSSWSPGGD
110 120 130 140 150
SAVPSGDDGL QCLGGDTPAG GAERWLQVSF GLFGSIRVNE FSRAKKANKR
160 170 180 190 200
GDWRDPVPRL VLHFSGSGFL AFYNCQMTWR FSSPVVSPAS DILSEKFHRG
210 220 230 240 250
QALEALGREQ PICYTLLDQR YFSGLGNIIK NEALFRAGIH PLSPGSLLGL
260 270 280 290 300
PRLEALVDHV VAFSADWLQG KFQGTRQHTQ IYQKEQCPAG HQVVRESLGP
310 320
PGGFQRLTWW CPQCQPRLSA DEPKQLQPS
Length:329
Mass (Da):36,392
Last modified:January 23, 2007 - v3
Checksum:i0D48CC7BEDA526EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC107065 Genomic DNA No translation available.
BN000316 mRNA Translation: CAE48362.1
RefSeqiNP_001013021.1, NM_001013003.1
XP_005209884.1, XM_005209827.3
XP_010806002.1, XM_010807700.2
UniGeneiBt.26303

Genome annotation databases

EnsembliENSBTAT00000006262; ENSBTAP00000006262; ENSBTAG00000004769
GeneIDi444987
KEGGibta:444987

Similar proteinsi

Entry informationi

Entry nameiNEIL2_BOVIN
AccessioniPrimary (citable) accession number: Q6IE77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 92 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health