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Q6IE77 (NEIL2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endonuclease 8-like 2

EC=3.2.2.-
EC=4.2.99.18
Alternative name(s):
DNA glycosylase/AP lyase Neil2
DNA-(apurinic or apyrimidinic site) lyase Neil2
Endonuclease VIII-like 2
Nei homolog 2
Short name=NEH2
Nei-like protein 2
Gene names
Name:NEIL2
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity.

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Enzyme regulation

Acetylation of Lys-50 leads to loss of DNA nicking activity By similarity.

Subunit structure

Binds EP300 By similarity.

Subcellular location

Nucleus By similarity.

Domain

The zinc-finger domain is important for DNA binding.

Sequence similarities

Belongs to the FPG family.

Contains 1 FPG-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 329328Endonuclease 8-like 2
PRO_0000170907

Regions

Zinc finger280 – 31637FPG-type

Sites

Active site21Schiff-base intermediate with DNA By similarity
Active site31Proton donor By similarity
Active site501Proton donor; for beta-elimination activity By similarity
Active site3061Proton donor; for delta-elimination activity By similarity
Binding site2271DNA By similarity

Amino acid modifications

Modified residue501N6-acetyllysine By similarity
Modified residue1491N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6IE77 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 0D48CC7BEDA526EA

FASTA32936,392
        10         20         30         40         50         60 
MPEGPSVRKF HHLVSPFVGQ QVVKTGGSSK KLNPTSFQSL WLQDSQVHGK KLFLRFDPDE 

        70         80         90        100        110        120 
EAVSLGNSLL SEPLREGEQK DKARHHQEAS DPSSWSPGGD SAVPSGDDGL QCLGGDTPAG 

       130        140        150        160        170        180 
GAERWLQVSF GLFGSIRVNE FSRAKKANKR GDWRDPVPRL VLHFSGSGFL AFYNCQMTWR 

       190        200        210        220        230        240 
FSSPVVSPAS DILSEKFHRG QALEALGREQ PICYTLLDQR YFSGLGNIIK NEALFRAGIH 

       250        260        270        280        290        300 
PLSPGSLLGL PRLEALVDHV VAFSADWLQG KFQGTRQHTQ IYQKEQCPAG HQVVRESLGP 

       310        320 
PGGFQRLTWW CPQCQPRLSA DEPKQLQPS 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of taurine cattle: a window to ruminant biology and evolution."
The bovine genome sequencing and analysis consortium
Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hereford.
[2]"Mapping of the GATA4, NEIL2, FDFT1 genes and CTSB-associated microsatellites to the centromeric region of BTA8."
Plis-Finarov A., Hudson H., Roe B., Ron M., Seroussi E.
Anim. Genet. 35:154-155(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC107065 Genomic DNA. No translation available.
BN000316 mRNA. Translation: CAE48362.1.
RefSeqNP_001013021.1. NM_001013003.1.
XP_005209884.1. XM_005209827.1.
UniGeneBt.26303.

3D structure databases

ProteinModelPortalQ6IE77.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000006262.

Proteomic databases

PRIDEQ6IE77.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000006262; ENSBTAP00000006262; ENSBTAG00000004769.
GeneID444987.
KEGGbta:444987.

Organism-specific databases

CTD252969.

Phylogenomic databases

eggNOGNOG73546.
GeneTreeENSGT00730000110955.
HOGENOMHOG000069913.
HOVERGENHBG082014.
InParanoidQ6IE77.
KOK10568.
OMAGHQVMKE.
OrthoDBEOG7Z69CR.
TreeFamTF331502.

Family and domain databases

InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SUPFAMSSF46946. SSF46946. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20831770.

Entry information

Entry nameNEIL2_BOVIN
AccessionPrimary (citable) accession number: Q6IE77
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 72 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families