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Q6IE77

- NEIL2_BOVIN

UniProt

Q6IE77 - NEIL2_BOVIN

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Protein
Endonuclease 8-like 2
Gene
NEIL2
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity.

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Enzyme regulationi

Acetylation of Lys-50 leads to loss of DNA nicking activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNA By similarity
Active sitei3 – 31Proton donor By similarity
Active sitei50 – 501Proton donor; for beta-elimination activity By similarity
Binding sitei227 – 2271DNA By similarity
Active sitei306 – 3061Proton donor; for delta-elimination activity By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri280 – 31637FPG-type
Add
BLAST

GO - Molecular functioni

  1. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
  2. damaged DNA binding Source: InterPro
  3. hydrolase activity, hydrolyzing N-glycosyl compounds Source: InterPro
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. base-excision repair Source: InterPro
  2. nucleotide-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Lyase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 8-like 2 (EC:3.2.2.-, EC:4.2.99.18)
Alternative name(s):
DNA glycosylase/AP lyase Neil2
DNA-(apurinic or apyrimidinic site) lyase Neil2
Endonuclease VIII-like 2
Nei homolog 2
Short name:
NEH2
Nei-like protein 2
Gene namesi
Name:NEIL2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 8

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB-SubCell
  3. spindle microtubule Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 329328Endonuclease 8-like 2
PRO_0000170907Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501N6-acetyllysine By similarity
Modified residuei149 – 1491N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ6IE77.

Interactioni

Subunit structurei

Binds EP300 By similarity.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000006262.

Structurei

3D structure databases

ProteinModelPortaliQ6IE77.

Family & Domainsi

Domaini

The zinc-finger domain is important for DNA binding.

Sequence similaritiesi

Belongs to the FPG family.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG73546.
GeneTreeiENSGT00730000110955.
HOGENOMiHOG000069913.
HOVERGENiHBG082014.
InParanoidiQ6IE77.
KOiK10568.
OMAiSVRKFHH.
OrthoDBiEOG7Z69CR.
TreeFamiTF331502.

Family and domain databases

InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6IE77-1 [UniParc]FASTAAdd to Basket

« Hide

MPEGPSVRKF HHLVSPFVGQ QVVKTGGSSK KLNPTSFQSL WLQDSQVHGK    50
KLFLRFDPDE EAVSLGNSLL SEPLREGEQK DKARHHQEAS DPSSWSPGGD 100
SAVPSGDDGL QCLGGDTPAG GAERWLQVSF GLFGSIRVNE FSRAKKANKR 150
GDWRDPVPRL VLHFSGSGFL AFYNCQMTWR FSSPVVSPAS DILSEKFHRG 200
QALEALGREQ PICYTLLDQR YFSGLGNIIK NEALFRAGIH PLSPGSLLGL 250
PRLEALVDHV VAFSADWLQG KFQGTRQHTQ IYQKEQCPAG HQVVRESLGP 300
PGGFQRLTWW CPQCQPRLSA DEPKQLQPS 329
Length:329
Mass (Da):36,392
Last modified:January 23, 2007 - v3
Checksum:i0D48CC7BEDA526EA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC107065 Genomic DNA. No translation available.
BN000316 mRNA. Translation: CAE48362.1.
RefSeqiNP_001013021.1. NM_001013003.1.
XP_005209884.1. XM_005209827.1.
UniGeneiBt.26303.

Genome annotation databases

EnsembliENSBTAT00000006262; ENSBTAP00000006262; ENSBTAG00000004769.
GeneIDi444987.
KEGGibta:444987.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC107065 Genomic DNA. No translation available.
BN000316 mRNA. Translation: CAE48362.1 .
RefSeqi NP_001013021.1. NM_001013003.1.
XP_005209884.1. XM_005209827.1.
UniGenei Bt.26303.

3D structure databases

ProteinModelPortali Q6IE77.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000006262.

Proteomic databases

PRIDEi Q6IE77.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000006262 ; ENSBTAP00000006262 ; ENSBTAG00000004769 .
GeneIDi 444987.
KEGGi bta:444987.

Organism-specific databases

CTDi 252969.

Phylogenomic databases

eggNOGi NOG73546.
GeneTreei ENSGT00730000110955.
HOGENOMi HOG000069913.
HOVERGENi HBG082014.
InParanoidi Q6IE77.
KOi K10568.
OMAi SVRKFHH.
OrthoDBi EOG7Z69CR.
TreeFami TF331502.

Miscellaneous databases

NextBioi 20831770.

Family and domain databases

InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
[Graphical view ]
Pfami PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of taurine cattle: a window to ruminant biology and evolution."
    The bovine genome sequencing and analysis consortium
    Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hereford.
  2. "Mapping of the GATA4, NEIL2, FDFT1 genes and CTSB-associated microsatellites to the centromeric region of BTA8."
    Plis-Finarov A., Hudson H., Roe B., Ron M., Seroussi E.
    Anim. Genet. 35:154-155(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiNEIL2_BOVIN
AccessioniPrimary (citable) accession number: Q6IE77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 73 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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