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Protein

Beta-secretase 2

Gene

Bace2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672 (By similarity).By similarity

Catalytic activityi

Broad endopeptidase specificity. Cleaves Glu-Val-Asn-Leu-|-Asp-Ala-Glu-Phe in the Swedish variant of Alzheimer's amyloid precursor protein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei106PROSITE-ProRule annotation1
Active sitei299PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Protein family/group databases

MEROPSiA01.041.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-secretase 2 (EC:3.4.23.45)
Alternative name(s):
Beta-site amyloid precursor protein cleaving enzyme 2
Short name:
Beta-site APP cleaving enzyme 2
Memapsin-1
Membrane-associated aspartic protease 1
Theta-secretase
Gene namesi
Name:Bace2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 11

Organism-specific databases

RGDi1303241. Bace2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini21 – 469ExtracellularSequence analysisAdd BLAST449
Transmembranei470 – 490HelicalSequence analysisAdd BLAST21
Topological domaini491 – 514CytoplasmicSequence analysisAdd BLAST24

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2331066.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000038364821 – 62By similarityAdd BLAST42
ChainiPRO_000038364963 – 514Beta-secretase 2Add BLAST452

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi229 ↔ 429By similarity
Disulfide bondi288 ↔ 453By similarity
Disulfide bondi340 ↔ 389By similarity
Glycosylationi362N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Undergoes autoproteolytic cleavage.By similarity
Glycosylated.By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ6IE75.
PRIDEiQ6IE75.

PTM databases

PhosphoSitePlusiQ6IE75.

Expressioni

Gene expression databases

BgeeiENSRNOG00000001953.
GenevisibleiQ6IE75. RN.

Interactioni

Subunit structurei

Monomer. Interacts ith RTN3 and RTN4 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002679.

Chemistry databases

BindingDBiQ6IE75.

Structurei

3D structure databases

ProteinModelPortaliQ6IE75.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 425Peptidase A1PROSITE-ProRule annotationAdd BLAST338

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000036572.
InParanoidiQ6IE75.
KOiK07747.
OMAiDNLQGDS.
OrthoDBiEOG091G0CNK.
PhylomeDBiQ6IE75.
TreeFamiTF329595.

Family and domain databases

CDDicd05473. beta_secretase_like. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR009119. BACE.
IPR009121. BACE2.
IPR033874. Memapsin-like.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PTHR13683:SF262. PTHR13683:SF262. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR01817. BACE2.
PR01815. BACEFAMILY.
PR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6IE75-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGALLRALLL PLLAQWLLRA VPVLAPAPFT LPLQVAGAAN HRASTVPGLG
60 70 80 90 100
TPELPRADGL ALALEPARAT ANFLAMVDNL QGDSGRGYYL EMLIGTPPQK
110 120 130 140 150
VRILVDTGSS NFAVAGAPHS YIDTYFDSES SSTYHSKGFE VTVKYTQGSW
160 170 180 190 200
TGFVGEDLVT IPKGFNSSFL VNIATIFESE NFFLPGIKWN GILGLAYAAL
210 220 230 240 250
AKPSSSLETF FDSLVAQAKI PDIFSMQMCG AGLPVAGSGT NGGSLVLGGI
260 270 280 290 300
EPSLYKGDIW YTPIKEEWYY QIEILKLEIG GQSLNLDCRE YNADKAIVDS
310 320 330 340 350
GTTLLRLPQK VFDAVVEAVA RTSLIPEFSD GFWTGAQLAC WTNSETPWAY
360 370 380 390 400
FPKISIYLRD ENASRSFRIT ILPQLYIQPM MGAGFNYECY RFGISSSTNA
410 420 430 440 450
LVIGATVMEG FYVVFDRAQR RVGFAVSPCA EIAGTTVSEI SGPFSTEDIA
460 470 480 490 500
SNCVPAQALN EPILWIVSYA LMSVCGAILL VLILLLLFPL HCRHAPRDPE
510
VVNDESSLVR HRWK
Length:514
Mass (Da):55,858
Last modified:July 5, 2004 - v1
Checksum:i70D31A970E2A0CDD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188K → R in AAI66597 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC166597 mRNA. Translation: AAI66597.1.
BN000318 mRNA. Translation: CAE48373.1.
RefSeqiNP_001002802.1. NM_001002802.1.
UniGeneiRn.38581.

Genome annotation databases

EnsembliENSRNOT00000002679; ENSRNOP00000002679; ENSRNOG00000001953.
GeneIDi288227.
KEGGirno:288227.
UCSCiRGD:1303241. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC166597 mRNA. Translation: AAI66597.1.
BN000318 mRNA. Translation: CAE48373.1.
RefSeqiNP_001002802.1. NM_001002802.1.
UniGeneiRn.38581.

3D structure databases

ProteinModelPortaliQ6IE75.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000002679.

Chemistry databases

BindingDBiQ6IE75.
ChEMBLiCHEMBL2331066.

Protein family/group databases

MEROPSiA01.041.

PTM databases

PhosphoSitePlusiQ6IE75.

Proteomic databases

PaxDbiQ6IE75.
PRIDEiQ6IE75.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000002679; ENSRNOP00000002679; ENSRNOG00000001953.
GeneIDi288227.
KEGGirno:288227.
UCSCiRGD:1303241. rat.

Organism-specific databases

CTDi25825.
RGDi1303241. Bace2.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
GeneTreeiENSGT00760000118929.
HOGENOMiHOG000036572.
InParanoidiQ6IE75.
KOiK07747.
OMAiDNLQGDS.
OrthoDBiEOG091G0CNK.
PhylomeDBiQ6IE75.
TreeFamiTF329595.

Miscellaneous databases

PROiQ6IE75.

Gene expression databases

BgeeiENSRNOG00000001953.
GenevisibleiQ6IE75. RN.

Family and domain databases

CDDicd05473. beta_secretase_like. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR009119. BACE.
IPR009121. BACE2.
IPR033874. Memapsin-like.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 1 hit.
PTHR13683:SF262. PTHR13683:SF262. 1 hit.
PfamiPF00026. Asp. 1 hit.
[Graphical view]
PRINTSiPR01817. BACE2.
PR01815. BACEFAMILY.
PR00792. PEPSIN.
SUPFAMiSSF50630. SSF50630. 1 hit.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBACE2_RAT
AccessioniPrimary (citable) accession number: Q6IE75
Secondary accession number(s): B2GVB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.