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Protein

PAB-dependent poly(A)-specific ribonuclease subunit PAN2

Gene

Pan2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA when the poly(A) stretch is bound by poly(A)-binding protein (PABP), which is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1.UniRule annotation

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.UniRule annotation

Enzyme regulationi

Positively regulated by the regulatory subunit PAN3.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
PAB-dependent poly(A)-specific ribonuclease subunit PAN2UniRule annotation (EC:3.1.13.4UniRule annotation)
Alternative name(s):
Inactive ubiquitin carboxyl-terminal hydrolase 52UniRule annotation
PAB1P-dependent poly(A)-nucleaseUniRule annotation
PAN deadenylation complex catalytic subunit 2UniRule annotation
Gene namesi
Name:Pan2
Synonyms:Usp52
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1303301. Pan2.

Subcellular locationi

  • CytoplasmP-body UniRule annotation
  • Nucleus UniRule annotation

  • Note: Shuttles between nucleus and cytoplasm.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12051205PAB-dependent poly(A)-specific ribonuclease subunit PAN2PRO_0000280523Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei784 – 7841PhosphoserineBy similarity
Modified residuei1189 – 11891PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6IE70.
PRIDEiQ6IE70.

PTM databases

PhosphoSiteiQ6IE70.

Interactioni

Subunit structurei

Interacts with the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039314.

Structurei

3D structure databases

ProteinModelPortaliQ6IE70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati153 – 19341WD 1Add
BLAST
Repeati195 – 23137WD 2Add
BLAST
Repeati244 – 28037WD 3Add
BLAST
Repeati328 – 36740WD 4Add
BLAST
Domaini516 – 924409USPUniRule annotationAdd
BLAST
Domaini975 – 1147173ExonucleaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. PAN2 subfamily.UniRule annotation
Contains 1 exonuclease domain.UniRule annotation
Contains 1 USP domain.UniRule annotation
Contains 4 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1275. Eukaryota.
COG0847. LUCA.
HOGENOMiHOG000230585.
HOVERGENiHBG089847.
InParanoidiQ6IE70.
KOiK12571.
OrthoDBiEOG7JHM4K.
PhylomeDBiQ6IE70.
TreeFamiTF105657.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.420.10. 1 hit.
HAMAPiMF_03182. PAN2.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR030843. PAN2.
IPR028881. PAN2_dom.
IPR012337. RNaseH-like_dom.
IPR028889. USP_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
PF13423. UCH_1. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6IE70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFEGLDPGL AEFSPAMHST LDPVLDAHLN PSLLQNVELD PEGVALEALP
60 70 80 90 100
VQESVHIMEG VYSELHSVVA EVGVPVSVSH FDLQEEMLWV GSHGGHATSF
110 120 130 140 150
FGPALERYSS FQVNGSDDIR QIQSLENGIL FLTKNNLKYM ARGGLIIFDY
160 170 180 190 200
LLDESEDMHS LLLADNNTLL VGGLQNHVLE IDLNTVQETQ KYAVETPGVT
210 220 230 240 250
IMRQTNRFFF CGHTSGKVSL RDLRSFKVEH EFDAFSGSLS DFDVHGNLLA
260 270 280 290 300
ACGFSSRLTG LACDRFLKVY DLRMMRAITP LQVHVDPAFL RFIPTYTSRL
310 320 330 340 350
AIISQSGQCQ FCEPTGLANP ADIFHVNPVG PLLMTFDVSA SKQALAFGDS
360 370 380 390 400
EGCVHLWTDS PEPSFNPYSR ETEFALPCLV DSLPALDWSQ DLLPLSLIPV
410 420 430 440 450
PLTTDALLSD WPAANSAPAP RRAPPVDAEI LRTMKKVGFI GYAPNPRTRL
460 470 480 490 500
RNQIPYRLKE SDHEFDNFSQ VTESPTGREE EPLHTVSKKY RKVTIKYSKL
510 520 530 540 550
GLEDFDFKHY NKTLFAGLEP HIPNAYCNCM IQVLYFLEPV RCLIQNHLCQ
560 570 580 590 600
KEFCLACELG FLFHMLDLSR GDPCQGSNFL RAFRTIPEAS ALGLILADSD
610 620 630 640 650
EASGKGSLAR LIQRWNRFIL TQLHQDMQEL EVPQAYRGAG GSFCSSGDSI
660 670 680 690 700
IGQLFSCEME NCSLCRCGSE TVRASSTLLF TLSYPEDKTG KNYDFAQVLK
710 720 730 740 750
RSICLEQNTQ AWCDSCEKYQ PTIQTRNIRH LPDILVINCE VNSSKEADFW
760 770 780 790 800
RLQAEVAFKI AVKKFGGEVK SKEFALADRK ELRSPEGFLC CSSMEELKNV
810 820 830 840 850
WLPFSIRMKM TKNKGLDVCN WADEHELSSL GTPSQWGPAR AEEEHGVYVY
860 870 880 890 900
DLMATVVHIL DSRTGGSLVA HIKVGETYHQ RKEGVTHQQW YLFNDFLIEP
910 920 930 940 950
IDKYEAVQFD MNWKVPAILY YVKRNLNSRY NLNSKNPIEA SVLLAEASLA
960 970 980 990 1000
RKQRKTHTTF IPLMLNEMPQ VGDLVGLDAE FVTLNEEEAE LRSDGTKSTI
1010 1020 1030 1040 1050
KPSQMSVARI TCVRGQGPNE GIPFIDDYIS TQEQVVDYLT QYSGIKPGDL
1060 1070 1080 1090 1100
DAKISSKHLT TLKSTYLKLR FLIDIGVKFV GHGLQKDFRV INLMVPKDQV
1110 1120 1130 1140 1150
LDTVYLFHMP RKRMISLRFL AWYFLDLKIQ GETHDSIEDA RTALQLYRKY
1160 1170 1180 1190 1200
LELSKNGAEP ESFHKVLKGL YEKGRKMDWK VPEPESQSSP KSKAGLRPGA

LGWVG
Length:1,205
Mass (Da):135,627
Last modified:July 5, 2004 - v1
Checksum:i525532F675D88D08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03055877 Genomic DNA. No translation available.
BN000323 mRNA. Translation: CAE48378.1.
RefSeqiNP_001008862.1. NM_001008862.1.
UniGeneiRn.9091.

Genome annotation databases

GeneIDi408200.
KEGGirno:408200.
UCSCiRGD:1303301. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03055877 Genomic DNA. No translation available.
BN000323 mRNA. Translation: CAE48378.1.
RefSeqiNP_001008862.1. NM_001008862.1.
UniGeneiRn.9091.

3D structure databases

ProteinModelPortaliQ6IE70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039314.

PTM databases

PhosphoSiteiQ6IE70.

Proteomic databases

PaxDbiQ6IE70.
PRIDEiQ6IE70.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi408200.
KEGGirno:408200.
UCSCiRGD:1303301. rat.

Organism-specific databases

CTDi9924.
RGDi1303301. Pan2.

Phylogenomic databases

eggNOGiKOG1275. Eukaryota.
COG0847. LUCA.
HOGENOMiHOG000230585.
HOVERGENiHBG089847.
InParanoidiQ6IE70.
KOiK12571.
OrthoDBiEOG7JHM4K.
PhylomeDBiQ6IE70.
TreeFamiTF105657.

Miscellaneous databases

NextBioi696390.
PROiQ6IE70.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.420.10. 1 hit.
HAMAPiMF_03182. PAN2.
InterProiIPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR030843. PAN2.
IPR028881. PAN2_dom.
IPR012337. RNaseH-like_dom.
IPR028889. USP_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
PF13423. UCH_1. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
SSF53098. SSF53098. 1 hit.
PROSITEiPS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "A genomic analysis of rat proteases and protease inhibitors."
    Puente X.S., Lopez-Otin C.
    Genome Res. 14:609-622(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiPAN2_RAT
AccessioniPrimary (citable) accession number: Q6IE70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Has probably no ubiquitin carboxyl terminal hydrolase activity since it lacks a conserved catalytic Cys at position 524.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.