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Protein

PAN2-PAN3 deadenylation complex catalytic subunit Pan2

Gene

Pan2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation.UniRule annotation

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.UniRule annotation

Cofactori

a divalent metal cationUniRule annotationNote: Binds 2 metal cations per subunit in the catalytic exonuclease domain.UniRule annotation

Enzyme regulationi

Positively regulated by the regulatory subunit PAN3.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi978Divalent metal cation; catalyticUniRule annotation1
Metal bindingi980Divalent metal cation; catalyticUniRule annotation1
Metal bindingi1087Divalent metal cation; catalyticUniRule annotation1
Metal bindingi1139Divalent metal cation; catalyticUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExonuclease, Hydrolase, Nuclease
Biological processmRNA processing
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
PAN2-PAN3 deadenylation complex catalytic subunit Pan2UniRule annotation (EC:3.1.13.4UniRule annotation)
Alternative name(s):
Inactive ubiquitin carboxyl-terminal hydrolase 52UniRule annotation
PAB1P-dependent poly(A)-specific ribonucleaseUniRule annotation
Poly(A)-nuclease deadenylation complex subunit 2UniRule annotation
Short name:
PAN deadenylation complex subunit 2UniRule annotation
Gene namesi
Name:Pan2UniRule annotation
Synonyms:Usp52
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1303301 Pan2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002805231 – 1205PAN2-PAN3 deadenylation complex catalytic subunit Pan2Add BLAST1205

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei784PhosphoserineBy similarity1
Modified residuei1189PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ6IE70
PRIDEiQ6IE70

PTM databases

iPTMnetiQ6IE70
PhosphoSitePlusiQ6IE70

Interactioni

Subunit structurei

Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts with ZFP36.UniRule annotation

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000039314

Structurei

3D structure databases

ProteinModelPortaliQ6IE70
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati153 – 193WD 1UniRule annotationAdd BLAST41
Repeati195 – 231WD 2UniRule annotationAdd BLAST37
Repeati244 – 280WD 3UniRule annotationAdd BLAST37
Repeati328 – 367WD 4UniRule annotationAdd BLAST40
Domaini485 – 924USPUniRule annotationAdd BLAST440
Domaini975 – 1147ExonucleaseUniRule annotationAdd BLAST173

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni368 – 484LinkerUniRule annotationAdd BLAST117

Domaini

Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.UniRule annotation
The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with PAN3.UniRule annotation

Sequence similaritiesi

Belongs to the peptidase C19 family. PAN2 subfamily.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1275 Eukaryota
COG0847 LUCA
HOGENOMiHOG000230585
HOVERGENiHBG089847
InParanoidiQ6IE70
KOiK12571
PhylomeDBiQ6IE70
TreeFamiTF105657

Family and domain databases

Gene3Di2.130.10.10, 1 hit
3.30.420.10, 1 hit
HAMAPiMF_03182 PAN2, 1 hit
InterProiView protein in InterPro
IPR013520 Exonuclease_RNaseT/DNA_pol3
IPR030843 PAN2
IPR028881 PAN2_dom
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
IPR028889 USP_dom
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00929 RNase_T, 1 hit
PF13423 UCH_1, 1 hit
SMARTiView protein in SMART
SM00479 EXOIII, 1 hit
SUPFAMiSSF50978 SSF50978, 1 hit
SSF53098 SSF53098, 1 hit
PROSITEiView protein in PROSITE
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q6IE70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFEGLDPGL AEFSPAMHST LDPVLDAHLN PSLLQNVELD PEGVALEALP
60 70 80 90 100
VQESVHIMEG VYSELHSVVA EVGVPVSVSH FDLQEEMLWV GSHGGHATSF
110 120 130 140 150
FGPALERYSS FQVNGSDDIR QIQSLENGIL FLTKNNLKYM ARGGLIIFDY
160 170 180 190 200
LLDESEDMHS LLLADNNTLL VGGLQNHVLE IDLNTVQETQ KYAVETPGVT
210 220 230 240 250
IMRQTNRFFF CGHTSGKVSL RDLRSFKVEH EFDAFSGSLS DFDVHGNLLA
260 270 280 290 300
ACGFSSRLTG LACDRFLKVY DLRMMRAITP LQVHVDPAFL RFIPTYTSRL
310 320 330 340 350
AIISQSGQCQ FCEPTGLANP ADIFHVNPVG PLLMTFDVSA SKQALAFGDS
360 370 380 390 400
EGCVHLWTDS PEPSFNPYSR ETEFALPCLV DSLPALDWSQ DLLPLSLIPV
410 420 430 440 450
PLTTDALLSD WPAANSAPAP RRAPPVDAEI LRTMKKVGFI GYAPNPRTRL
460 470 480 490 500
RNQIPYRLKE SDHEFDNFSQ VTESPTGREE EPLHTVSKKY RKVTIKYSKL
510 520 530 540 550
GLEDFDFKHY NKTLFAGLEP HIPNAYCNCM IQVLYFLEPV RCLIQNHLCQ
560 570 580 590 600
KEFCLACELG FLFHMLDLSR GDPCQGSNFL RAFRTIPEAS ALGLILADSD
610 620 630 640 650
EASGKGSLAR LIQRWNRFIL TQLHQDMQEL EVPQAYRGAG GSFCSSGDSI
660 670 680 690 700
IGQLFSCEME NCSLCRCGSE TVRASSTLLF TLSYPEDKTG KNYDFAQVLK
710 720 730 740 750
RSICLEQNTQ AWCDSCEKYQ PTIQTRNIRH LPDILVINCE VNSSKEADFW
760 770 780 790 800
RLQAEVAFKI AVKKFGGEVK SKEFALADRK ELRSPEGFLC CSSMEELKNV
810 820 830 840 850
WLPFSIRMKM TKNKGLDVCN WADEHELSSL GTPSQWGPAR AEEEHGVYVY
860 870 880 890 900
DLMATVVHIL DSRTGGSLVA HIKVGETYHQ RKEGVTHQQW YLFNDFLIEP
910 920 930 940 950
IDKYEAVQFD MNWKVPAILY YVKRNLNSRY NLNSKNPIEA SVLLAEASLA
960 970 980 990 1000
RKQRKTHTTF IPLMLNEMPQ VGDLVGLDAE FVTLNEEEAE LRSDGTKSTI
1010 1020 1030 1040 1050
KPSQMSVARI TCVRGQGPNE GIPFIDDYIS TQEQVVDYLT QYSGIKPGDL
1060 1070 1080 1090 1100
DAKISSKHLT TLKSTYLKLR FLIDIGVKFV GHGLQKDFRV INLMVPKDQV
1110 1120 1130 1140 1150
LDTVYLFHMP RKRMISLRFL AWYFLDLKIQ GETHDSIEDA RTALQLYRKY
1160 1170 1180 1190 1200
LELSKNGAEP ESFHKVLKGL YEKGRKMDWK VPEPESQSSP KSKAGLRPGA

LGWVG
Length:1,205
Mass (Da):135,627
Last modified:July 5, 2004 - v1
Checksum:i525532F675D88D08
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03055877 Genomic DNA No translation available.
BN000323 mRNA Translation: CAE48378.1
RefSeqiNP_001008862.1, NM_001008862.1
UniGeneiRn.9091

Genome annotation databases

GeneIDi408200
KEGGirno:408200
UCSCiRGD:1303301 rat

Similar proteinsi

Entry informationi

Entry nameiPAN2_RAT
AccessioniPrimary (citable) accession number: Q6IE70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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