Q6IE52 (MUG2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Murinoglobulin-2

Gene names

Name:

Mug2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	A proteinase activates the inhibitor by specific proteolysis in the bait region, which, by an unknown mechanism leads to reaction at the cysteinyl-glutamyl internal thiol ester site and to a conformational change, whereby the proteinase is trapped and/or covalently bound to the inhibitor. While in the tetrameric proteinase inhibitors steric inhibition is sufficiently strong, monomeric forms need a covalent linkage between the activated glutamyl residue of the original thiol ester and a terminal amino group of a lysine or another nucleophilic group on the proteinase, for inhibition to be effective.
Subunit structure	Monomer By similarity. UniProtKB P14046
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]

Ontologies

Keywords
Cellular component	Secreted
Domain	Bait region Signal
Molecular function	Protease inhibitor Serine protease inhibitor
PTM	Disulfide bond Glycoprotein Thioester bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of endopeptidase activity Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular space Inferred from electronic annotation. Source: InterPro
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 1448	1424	Murinoglobulin-2		PRO_0000271252
Regions
	Region	678 – 709	32	Bait region By similarity UniProtKB P28665
Amino acid modifications
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	295	1	N-linked (GlcNAc...) Potential
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
	Glycosylation	387	1	N-linked (GlcNAc...) Potential
	Glycosylation	502	1	N-linked (GlcNAc...) Potential
	Glycosylation	751	1	N-linked (GlcNAc...) Potential
	Glycosylation	846	1	N-linked (GlcNAc...) Potential
	Glycosylation	968	1	N-linked (GlcNAc...) Potential
	Glycosylation	1114	1	N-linked (GlcNAc...) Potential
	Glycosylation	1285	1	N-linked (GlcNAc...) Potential
	Glycosylation	1398	1	N-linked (GlcNAc...) Potential
	Disulfide bond	48 ↔ 86		By similarity UniProtKB P01023
	Disulfide bond	245 ↔ 277		By similarity UniProtKB P01023
	Disulfide bond	263 ↔ 289		By similarity UniProtKB P01023
	Disulfide bond	462 ↔ 556		By similarity UniProtKB P01023
	Disulfide bond	588 ↔ 748		By similarity UniProtKB P01023
	Disulfide bond	636 ↔ 681		By similarity UniProtKB P01023
	Disulfide bond	824 ↔ 860		By similarity UniProtKB P01023
	Disulfide bond	898 ↔ 1295		By similarity UniProtKB P01023
	Disulfide bond	1056 ↔ 1101		By similarity UniProtKB P01023
	Disulfide bond	1326 ↔ 1441		By similarity UniProtKB P01023
	Cross-link	949 ↔ 952		Isoglutamyl cysteine thioester (Cys-Gln) By similarity UniProtKB P01023

Sequences

	Sequence		Length	Mass (Da)
	Q6IE52 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 7690C1C75F7D75B0	FASTA	1,448	161,589
	10 20 30 40 50 60 MWKNREAQLC LFSVLLAFLP SASLLFGNSK YMVLVPSQLY TETPEKICLH LYHLNETVTV 70 80 90 100 110 120 TASLISQRGT RKLFDEPVVD KDLFHCVSFI PRLPSSEEEE SLDINIEGAK HKFSKRHVVL 130 140 150 160 170 180 VKNKESVVFV QTDKPMYKPG QSVKFRVVSM DKNLYPLKEL VQDPKMNRIM QWQDVKTENG 190 200 210 220 230 240 LKQLSFSLSA EPIQGPYKIV VLKQSGVKEE HSFTVMEFVL PRFGVDVKVP NAISVYDEII 250 260 270 280 290 300 SVTACDRYTY GKPVLGRVKV RLCHGNPSFS SETKSACKEE NSQLDNNGCS TQEVNITEFQ 310 320 330 340 350 360 LKENYLKVRQ AFHVNATVTE EGTGMEFGGY GRIEVERTRN KFLFLKADSH FRHGIPFFVK 370 380 390 400 410 420 VRLVDIKGDP IPNEQVFIKA QEAGYTNATT TDQHGLAKFS IDTSSISGYS LNIKVYHKEE 430 440 450 460 470 480 NLCIHSSCTA ERHAEAHHTA YAVYSLSKSY IYLDTEAGVL PCNQIHTVQA HFILKGQVLG 490 500 510 520 530 540 VLPQIVFHYL VMAQGSILQT GNHTHQVESG APVQGNFALE IPVEFSMVPM AKMLIYTILP 550 560 570 580 590 600 DGEVIADSVK FQVEKCLRNK VHLSFSPSQS LPASQTHMRV TASPQSLCGL RAVDQSVLLL 610 620 630 640 650 660 RPEAELSPSL IYDLPGMQDS NFIPRSHHPF EDEDDCLMYQ PRDTEELTYS VPYGREKDVY 670 680 690 700 710 720 RYVDMGLTAF TNLKIKHPTY CYDLPKEPPR KDPPRKDPEP KDTVVETIRN YFPETWVWDL 730 740 750 760 770 780 VTVSSSGVTE VEMTVPDTIT EWKAGALCLS NDTGLGLSSV ATLQAFQPFF VELTMPYSVI 790 800 810 820 830 840 RGEAFTLKAT VMNYLPTSLQ MTVQLEASPD FTAVPVGNDQ DSYCLGANGR HTSSWLVTPK 850 860 870 880 890 900 SLGNVNFSVS VEAQQSPEPC GSEVATVPET GRKDTVIKVL IVEPEGIKKE HTFSSLLCAS 910 920 930 940 950 960 DAELSETLSL LLPPRVVKDS ARAHFSVMGD ILSSAIKNTQ NLIQMPYGCG EQNMVLFAPN 970 980 990 1000 1010 1020 IYVLKYLNET QQLTENIKSK ALGYLRAGYQ RELNYKHKDG SYSAFGDHNG QGQGNTWLTA 1030 1040 1050 1060 1070 1080 FVLKSFAQAR AFIFIDESHI TDAFTWLSKQ QKDSGCFRSS GSLFNNAMKG GVDDEITLSA 1090 1100 1110 1120 1130 1140 YITMALLESS LPPVVSKALG CLEASWETIE QGRNGSFVYT KALMAYAFTL AGNQEKRNEI 1150 1160 1170 1180 1190 1200 LKSLDKEAIK EDNSIHWERP QKPMKSEHYL YTPQASSVEV EMSAYVVLAR LTAHPAPSPE 1210 1220 1230 1240 1250 1260 DLALSTGTIK WLTKQQNSHG GFSSTQDTVV ALDALSKYGA ATFSKSQKTP SVTVQSSGSF 1270 1280 1290 1300 1310 1320 SQKFQVDKSN RLLLQQVSLP DIPGNYTIRV SGEGCVYAQT TLRYNLPLEK QQPAFALKVK 1330 1340 1350 1360 1370 1380 TVPLTCNNPK GQNSFQISLE ISYTGSRPAS NMVIADVKML SGFIPLKPTV KKLERLEHVS 1390 1400 1410 1420 1430 1440 RTEVTTNNVL LYLDQVTNQT LSFSFIIQQD IPVKNLQPAI VKVYDYYETD EVAFAEYSSP CSSDKQNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[2]	Lubec G., Kang S.U. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 873-878, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain.
[3]	"A genomic analysis of rat proteases and protease inhibitors." Puente X.S., Lopez-Otin C. Genome Res. 14:609-622(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC113675 Genomic DNA. No translation available. BN000341 mRNA. Translation: CAE51393.1.
IPI	IPI00476749.
RefSeq	NP_001002826.1. NM_001002826.1.
UniGene	Rn.198690.
3D structure databases
ProteinModelPortal	Q6IE52.
SMR	Q6IE52. Positions 125-221, 1315-1447.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000040516.
Proteomic databases
PRIDE	Q6IE52.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	408236.
KEGG	rno:408236.
UCSC	RGD:1302962. rat.
Organism-specific databases
CTD	17837.
RGD	1302962. Mug2.
Phylogenomic databases
HOGENOM	HOG000220939.
HOVERGEN	HBG000039.
Gene expression databases
ArrayExpress	Q6IE52.
Genevestigator	Q6IE52.
Family and domain databases
Gene3D	2.60.40.690. 1 hit.
InterPro	IPR009048. A-macroglobulin_rcpt-bd. IPR011626. A2M_comp. IPR002890. A2M_N. IPR011625. A2M_N_2. IPR001599. Macroglobln_a2. IPR019742. MacrogloblnA2_CS. IPR019565. MacrogloblnA2_thiol-ester-bond. IPR008930. Terpenoid_cyclase/PrenylTrfase. IPR010916. TonB_box_CS. [Graphical view]
Pfam	PF00207. A2M. 1 hit. PF07678. A2M_comp. 1 hit. PF01835. A2M_N. 1 hit. PF07703. A2M_N_2. 1 hit. PF07677. A2M_recep. 1 hit. PF10569. Thiol-ester_cl. 1 hit. [Graphical view]
SUPFAM	SSF49410. AM_receptor_bind. 1 hit. SSF48239. Terp_cyc_toroid. 1 hit.
PROSITE	PS00477. ALPHA_2_MACROGLOBULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	696498.

Entry information

Entry name

MUG2_RAT

Accession

Primary (citable) accession number: Q6IE52

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program