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Protein

GRAM domain-containing protein 4

Gene

GRAMD4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role as a mediator of E2F1-induced apoptosis in the absence of p53/TP53.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
GRAM domain-containing protein 4
Alternative name(s):
Death-inducing protein
Gene namesi
Name:GRAMD4
Synonyms:DIP, KIAA0767
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

HGNCiHGNC:29113. GRAMD4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei240 – 26021HelicalSequence analysisAdd
BLAST
Transmembranei334 – 35421HelicalSequence analysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162390212.

Polymorphism and mutation databases

BioMutaiGRAMD4.
DMDMi74748754.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 578578GRAM domain-containing protein 4PRO_0000328742Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241PhosphoserineCombined sources
Modified residuei28 – 281PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ6IC98.
MaxQBiQ6IC98.
PaxDbiQ6IC98.
PeptideAtlasiQ6IC98.
PRIDEiQ6IC98.

PTM databases

iPTMnetiQ6IC98.
PhosphoSiteiQ6IC98.

Expressioni

Tissue specificityi

Expressed in lung and in primary lung squamous cell carcinoma (LSCC).1 Publication

Inductioni

Up-regulated in the mitochondria by E2F1 after addition of 4-hydroxytamoxifen (at protein level).2 Publications

Gene expression databases

BgeeiENSG00000075240.
CleanExiHS_GRAMD4.
ExpressionAtlasiQ6IC98. baseline and differential.
GenevisibleiQ6IC98. HS.

Organism-specific databases

HPAiHPA029502.

Interactioni

Protein-protein interaction databases

BioGridi116766. 5 interactions.
IntActiQ6IC98. 2 interactions.
STRINGi9606.ENSP00000354313.

Structurei

3D structure databases

ProteinModelPortaliQ6IC98.
SMRiQ6IC98. Positions 445-567.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini445 – 52379GRAMAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili83 – 14361Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 GRAM domain.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEG4. Eukaryota.
ENOG410ZXGG. LUCA.
GeneTreeiENSGT00390000010968.
HOGENOMiHOG000060209.
HOVERGENiHBG098114.
InParanoidiQ6IC98.
OMAiVKFFLID.
OrthoDBiEOG091G06UI.
PhylomeDBiQ6IC98.
TreeFamiTF320445.

Family and domain databases

InterProiIPR004182. GRAM.
[Graphical view]
PfamiPF02893. GRAM. 1 hit.
[Graphical view]
SMARTiSM00568. GRAM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6IC98-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLRRLDKIRF RGHKRDDFLD LAESPNASDT ECSDEIPLKV PRTSPRDSEE
60 70 80 90 100
LRDPAGPGTL IMATGVQDFN RTEFDRLNEI KGHLEIALLE KHFLQEELRK
110 120 130 140 150
LREETNAEML RQELDRERQR RMELEQKVQE VLKARTEEQM AQQPPKGQAQ
160 170 180 190 200
ASNGAERRSQ GLSSRLQKWF YERFGEYVED FRFQPEENTV ETEEPLSARR
210 220 230 240 250
LTENMRRLKR GAKPVTNFVK NLSALSDWYS VYTSAIAFTV YMNAVWHGWA
260 270 280 290 300
IPLFLFLAIL RLSLNYLIAR GWRIQWSIVP EVSEPVEPPK EDLTVSEKFQ
310 320 330 340 350
LVLDVAQKAQ NLFGKMADIL EKIKNLFMWV QPEITQKLYV ALWAAFLASC
360 370 380 390 400
FFPYRLVGLA VGLYAGIKFF LIDFIFKRCP RLRAKYDTPY IIWRSLPTDP
410 420 430 440 450
QLKERSSAAV SRRLQTTSSR SYVPSAPAGL GKEEDAGRFH STKKGNFHEI
460 470 480 490 500
FNLTENERPL AVCENGWRCC LINRDRKMPT DYIRNGVLYV TENYLCFESS
510 520 530 540 550
KSGSSKRNKV IKLVDITDIQ KYKVLSVLPG SGMGIAVSTP STQKPLVFGA
560 570
MVHRDEAFET ILSQYIKITS AAASGGDS
Length:578
Mass (Da):66,408
Last modified:July 5, 2004 - v1
Checksum:i7CE03B34F979D93D
GO
Isoform 2 (identifier: Q6IC98-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-477: Missing.

Note: No experimental confirmation available.
Show »
Length:101
Mass (Da):10,923
Checksum:i4C2105D694206AF1
GO

Sequence cautioni

The sequence BAA34487 differs from that shown. Reason: Erroneous initiation. Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti159 – 1591S → I.1 Publication
Corresponds to variant rs36211078 [ dbSNP | Ensembl ].
VAR_042505

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 477477Missing in isoform 2. 1 PublicationVSP_032771Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018310 mRNA. Translation: BAA34487.2. Different initiation.
CR456470 mRNA. Translation: CAG30356.1.
AL096766, AL021392 Genomic DNA. Translation: CAI18820.1.
AL021392 Genomic DNA. Translation: CAI20966.1.
AL021392 Genomic DNA. Translation: CAP58794.1.
AL021392 Genomic DNA. Translation: CAP58795.1.
AL096766 Genomic DNA. Translation: CAQ07606.1.
CH471138 Genomic DNA. Translation: EAW73432.1.
BC080189 mRNA. Translation: AAH80189.1.
BC129837 mRNA. Translation: AAI29838.1.
CCDSiCCDS33672.1. [Q6IC98-1]
RefSeqiNP_055939.1. NM_015124.4. [Q6IC98-1]
XP_006724233.1. XM_006724170.3. [Q6IC98-1]
UniGeneiHs.475150.
Hs.547593.

Genome annotation databases

EnsembliENST00000361034; ENSP00000354313; ENSG00000075240. [Q6IC98-1]
ENST00000406902; ENSP00000385689; ENSG00000075240. [Q6IC98-1]
ENST00000408031; ENSP00000385851; ENSG00000075240. [Q6IC98-2]
GeneIDi23151.
KEGGihsa:23151.
UCSCiuc003bhx.4. human. [Q6IC98-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018310 mRNA. Translation: BAA34487.2. Different initiation.
CR456470 mRNA. Translation: CAG30356.1.
AL096766, AL021392 Genomic DNA. Translation: CAI18820.1.
AL021392 Genomic DNA. Translation: CAI20966.1.
AL021392 Genomic DNA. Translation: CAP58794.1.
AL021392 Genomic DNA. Translation: CAP58795.1.
AL096766 Genomic DNA. Translation: CAQ07606.1.
CH471138 Genomic DNA. Translation: EAW73432.1.
BC080189 mRNA. Translation: AAH80189.1.
BC129837 mRNA. Translation: AAI29838.1.
CCDSiCCDS33672.1. [Q6IC98-1]
RefSeqiNP_055939.1. NM_015124.4. [Q6IC98-1]
XP_006724233.1. XM_006724170.3. [Q6IC98-1]
UniGeneiHs.475150.
Hs.547593.

3D structure databases

ProteinModelPortaliQ6IC98.
SMRiQ6IC98. Positions 445-567.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116766. 5 interactions.
IntActiQ6IC98. 2 interactions.
STRINGi9606.ENSP00000354313.

PTM databases

iPTMnetiQ6IC98.
PhosphoSiteiQ6IC98.

Polymorphism and mutation databases

BioMutaiGRAMD4.
DMDMi74748754.

Proteomic databases

EPDiQ6IC98.
MaxQBiQ6IC98.
PaxDbiQ6IC98.
PeptideAtlasiQ6IC98.
PRIDEiQ6IC98.

Protocols and materials databases

DNASUi23151.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361034; ENSP00000354313; ENSG00000075240. [Q6IC98-1]
ENST00000406902; ENSP00000385689; ENSG00000075240. [Q6IC98-1]
ENST00000408031; ENSP00000385851; ENSG00000075240. [Q6IC98-2]
GeneIDi23151.
KEGGihsa:23151.
UCSCiuc003bhx.4. human. [Q6IC98-1]

Organism-specific databases

CTDi23151.
GeneCardsiGRAMD4.
HGNCiHGNC:29113. GRAMD4.
HPAiHPA029502.
MIMi613691. gene.
neXtProtiNX_Q6IC98.
PharmGKBiPA162390212.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEG4. Eukaryota.
ENOG410ZXGG. LUCA.
GeneTreeiENSGT00390000010968.
HOGENOMiHOG000060209.
HOVERGENiHBG098114.
InParanoidiQ6IC98.
OMAiVKFFLID.
OrthoDBiEOG091G06UI.
PhylomeDBiQ6IC98.
TreeFamiTF320445.

Miscellaneous databases

GenomeRNAii23151.
PROiQ6IC98.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000075240.
CleanExiHS_GRAMD4.
ExpressionAtlasiQ6IC98. baseline and differential.
GenevisibleiQ6IC98. HS.

Family and domain databases

InterProiIPR004182. GRAM.
[Graphical view]
PfamiPF02893. GRAM. 1 hit.
[Graphical view]
SMARTiSM00568. GRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGRAM4_HUMAN
AccessioniPrimary (citable) accession number: Q6IC98
Secondary accession number(s): A9IN51
, A9IN57, Q68EN0, Q9UGE6, Q9Y4B9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.