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Q6IBS0

- TWF2_HUMAN

UniProt

Q6IBS0 - TWF2_HUMAN

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Protein

Twinfilin-2

Gene

TWF2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia (By similarity).By similarity

GO - Molecular functioni

  1. actin monomer binding Source: BHF-UCL
  2. ATP binding Source: UniProtKB
  3. phosphatidylinositol-4,5-bisphosphate binding Source: BHF-UCL
  4. poly(A) RNA binding Source: UniProtKB
  5. protein kinase C binding Source: BHF-UCL

GO - Biological processi

  1. barbed-end actin filament capping Source: BHF-UCL
  2. cell projection organization Source: UniProtKB-KW
  3. cellular response to growth factor stimulus Source: BHF-UCL
  4. cellular response to retinoic acid Source: BHF-UCL
  5. negative regulation of actin filament polymerization Source: BHF-UCL
  6. positive regulation of axon extension Source: BHF-UCL
  7. positive regulation of lamellipodium assembly Source: BHF-UCL
  8. positive regulation of neuron projection development Source: BHF-UCL
  9. regulation of actin cytoskeleton organization Source: BHF-UCL
  10. regulation of microvillus length Source: BHF-UCL
  11. sequestering of actin monomers Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Twinfilin-2
Alternative name(s):
A6-related protein
Short name:
hA6RP
Protein tyrosine kinase 9-like
Twinfilin-1-like protein
Gene namesi
Name:TWF2
Synonyms:PTK9L
ORF Names:MSTP011
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 3

Organism-specific databases

HGNCiHGNC:9621. TWF2.

Subcellular locationi

Cytoplasmcytoskeleton 1 Publication. Cytoplasmperinuclear region 1 Publication. Cell projectionstereocilium By similarity
Note: Perinuclear and G-actin-rich cortical actin structure sublocalization.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. cytoskeleton Source: UniProtKB-KW
  3. extracellular vesicular exosome Source: UniProt
  4. filopodium Source: BHF-UCL
  5. growth cone Source: BHF-UCL
  6. lamellipodium Source: BHF-UCL
  7. myofibril Source: BHF-UCL
  8. perinuclear region of cytoplasm Source: BHF-UCL
  9. stereocilium Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA162407429.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed3 Publications
Chaini2 – 349348Twinfilin-2PRO_0000233136Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine3 Publications
Modified residuei14 – 141N6-acetyllysine1 Publication
Modified residuei309 – 3091Phosphotyrosine2 Publications
Modified residuei349 – 3491Phosphoserine2 Publications

Post-translational modificationi

In vitro, phosphorylated by PRKCZ, CK2 and SRC.4 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ6IBS0.
PaxDbiQ6IBS0.
PeptideAtlasiQ6IBS0.
PRIDEiQ6IBS0.

2D gel databases

OGPiQ9Y3F5.

PTM databases

PhosphoSiteiQ6IBS0.

Expressioni

Tissue specificityi

Ubiquitously expressed (at protein level).1 Publication

Gene expression databases

BgeeiQ6IBS0.
CleanExiHS_TWF2.
ExpressionAtlasiQ6IBS0. baseline and differential.
GenevestigatoriQ6IBS0.

Organism-specific databases

HPAiHPA053874.

Interactioni

Subunit structurei

Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF1 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated. Interacts with MYO7A (By similarity).By similarity

Protein-protein interaction databases

BioGridi116472. 51 interactions.
IntActiQ6IBS0. 6 interactions.
MINTiMINT-1414584.
STRINGi9606.ENSP00000303908.

Structurei

Secondary structure

1
349
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi11 – 2212Combined sources
Beta strandi26 – 338Combined sources
Beta strandi36 – 438Combined sources
Helixi49 – 579Combined sources
Helixi58 – 603Combined sources
Beta strandi67 – 7711Combined sources
Beta strandi80 – 889Combined sources
Helixi95 – 11218Combined sources
Helixi114 – 1163Combined sources
Beta strandi117 – 1259Combined sources
Helixi126 – 1294Combined sources
Helixi131 – 1366Combined sources
Helixi184 – 19411Combined sources
Beta strandi199 – 2068Combined sources
Turni207 – 2104Combined sources
Beta strandi211 – 2166Combined sources
Helixi222 – 2287Combined sources
Beta strandi231 – 2333Combined sources
Beta strandi235 – 24511Combined sources
Beta strandi248 – 25811Combined sources
Helixi261 – 2633Combined sources
Helixi266 – 28520Combined sources
Beta strandi291 – 2988Combined sources
Helixi300 – 3023Combined sources
Helixi305 – 3128Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2VACX-ray1.70A6-137[»]
2W0IX-ray1.80A181-313[»]
ProteinModelPortaliQ6IBS0.
SMRiQ6IBS0. Positions 6-137, 181-313.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6IBS0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 139136ADF-H 1PROSITE-ProRule annotationAdd
BLAST
Domaini177 – 313137ADF-H 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ADF-H domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG263290.
GeneTreeiENSGT00530000063868.
HOGENOMiHOG000168296.
HOVERGENiHBG000848.
InParanoidiQ6IBS0.
KOiK08870.
OMAiHATPELK.
OrthoDBiEOG79GT6W.
PhylomeDBiQ6IBS0.
TreeFamiTF352598.

Family and domain databases

Gene3Di3.40.20.10. 2 hits.
InterProiIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR028458. Twinfilin.
[Graphical view]
PANTHERiPTHR13759. PTHR13759. 1 hit.
PfamiPF00241. Cofilin_ADF. 2 hits.
[Graphical view]
SMARTiSM00102. ADF. 2 hits.
[Graphical view]
PROSITEiPS51263. ADF_H. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6IBS0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAHQTGIHAT EELKEFFAKA RAGSVRLIKV VIEDEQLVLG ASQEPVGRWD
60 70 80 90 100
QDYDRAVLPL LDAQQPCYLL YRLDSQNAQG FEWLFLAWSP DNSPVRLKML
110 120 130 140 150
YAATRATVKK EFGGGHIKDE LFGTVKDDLS FAGYQKHLSS CAAPAPLTSA
160 170 180 190 200
ERELQQIRIN EVKTEISVES KHQTLQGLAF PLQPEAQRAL QQLKQKMVNY
210 220 230 240 250
IQMKLDLERE TIELVHTEPT DVAQLPSRVP RDAARYHFFL YKHTHEGDPL
260 270 280 290 300
ESVVFIYSMP GYKCSIKERM LYSSCKSRLL DSVEQDFHLE IAKKIEIGDG
310 320 330 340
AELTAEFLYD EVHPKQHAFK QAFAKPKGPG GKRGHKRLIR GPGENGDDS
Length:349
Mass (Da):39,548
Last modified:May 2, 2006 - v2
Checksum:i635EF8C04EFCE92B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti110 – 1101K → R in CAG33013. 1 PublicationCurated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti72 – 721R → C.1 Publication
Corresponds to variant rs35114109 [ dbSNP | Ensembl ].
VAR_042407
Natural varianti76 – 761Q → R.1 Publication
Corresponds to variant rs35711542 [ dbSNP | Ensembl ].
VAR_042408
Natural varianti103 – 1031A → T in a lung neuroendocrine carcinoma sample; somatic mutation. 1 Publication
VAR_042409

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17169 mRNA. Translation: CAB38055.1.
AL136773 mRNA. Translation: CAB66707.1.
AF109365 mRNA. Translation: AAQ13513.1.
CR456732 mRNA. Translation: CAG33013.1.
CR533520 mRNA. Translation: CAG38551.1.
BC000327 mRNA. Translation: AAH00327.1.
BC003161 mRNA. Translation: AAH03161.1.
BC016452 mRNA. Translation: AAH16452.1.
CCDSiCCDS2849.1.
PIRiT46362.
RefSeqiNP_009215.1. NM_007284.3.
UniGeneiHs.436439.

Genome annotation databases

EnsembliENST00000305533; ENSP00000303908; ENSG00000247596.
GeneIDi11344.
KEGGihsa:11344.
UCSCiuc003ddd.3. human.

Polymorphism databases

DMDMi94730596.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17169 mRNA. Translation: CAB38055.1 .
AL136773 mRNA. Translation: CAB66707.1 .
AF109365 mRNA. Translation: AAQ13513.1 .
CR456732 mRNA. Translation: CAG33013.1 .
CR533520 mRNA. Translation: CAG38551.1 .
BC000327 mRNA. Translation: AAH00327.1 .
BC003161 mRNA. Translation: AAH03161.1 .
BC016452 mRNA. Translation: AAH16452.1 .
CCDSi CCDS2849.1.
PIRi T46362.
RefSeqi NP_009215.1. NM_007284.3.
UniGenei Hs.436439.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2VAC X-ray 1.70 A 6-137 [» ]
2W0I X-ray 1.80 A 181-313 [» ]
ProteinModelPortali Q6IBS0.
SMRi Q6IBS0. Positions 6-137, 181-313.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 116472. 51 interactions.
IntActi Q6IBS0. 6 interactions.
MINTi MINT-1414584.
STRINGi 9606.ENSP00000303908.

PTM databases

PhosphoSitei Q6IBS0.

Polymorphism databases

DMDMi 94730596.

2D gel databases

OGPi Q9Y3F5.

Proteomic databases

MaxQBi Q6IBS0.
PaxDbi Q6IBS0.
PeptideAtlasi Q6IBS0.
PRIDEi Q6IBS0.

Protocols and materials databases

DNASUi 11344.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000305533 ; ENSP00000303908 ; ENSG00000247596 .
GeneIDi 11344.
KEGGi hsa:11344.
UCSCi uc003ddd.3. human.

Organism-specific databases

CTDi 11344.
GeneCardsi GC03M052262.
HGNCi HGNC:9621. TWF2.
HPAi HPA053874.
MIMi 607433. gene.
neXtProti NX_Q6IBS0.
PharmGKBi PA162407429.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG263290.
GeneTreei ENSGT00530000063868.
HOGENOMi HOG000168296.
HOVERGENi HBG000848.
InParanoidi Q6IBS0.
KOi K08870.
OMAi HATPELK.
OrthoDBi EOG79GT6W.
PhylomeDBi Q6IBS0.
TreeFami TF352598.

Miscellaneous databases

EvolutionaryTracei Q6IBS0.
GeneWikii TWF2.
GenomeRNAii 11344.
NextBioi 43112.
PROi Q6IBS0.
SOURCEi Search...

Gene expression databases

Bgeei Q6IBS0.
CleanExi HS_TWF2.
ExpressionAtlasi Q6IBS0. baseline and differential.
Genevestigatori Q6IBS0.

Family and domain databases

Gene3Di 3.40.20.10. 2 hits.
InterProi IPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR028458. Twinfilin.
[Graphical view ]
PANTHERi PTHR13759. PTHR13759. 1 hit.
Pfami PF00241. Cofilin_ADF. 2 hits.
[Graphical view ]
SMARTi SM00102. ADF. 2 hits.
[Graphical view ]
PROSITEi PS51263. ADF_H. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression and characterization of an A6 related protein."
    Rohwer A., Kittstein W., Marks F., Gschwendt M.
    Eur. J. Biochem. 263:518-525(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, PHOSPHORYLATION.
    Tissue: Keratinocyte.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Aorta.
  4. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney, Lung and Placenta.
  6. Bienvenut W.V., Vousden K.H., Lukashchuk N.
    Submitted (MAR-2008) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-19 AND 279-293, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Lung carcinoma.
  7. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-309, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-309 AND SER-349, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  11. "Lysine acetylation targets protein complexes and co-regulates major cellular functions."
    Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
    Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2 AND LYS-14, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-349, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] CYS-72; ARG-76 AND THR-103.

Entry informationi

Entry nameiTWF2_HUMAN
AccessioniPrimary (citable) accession number: Q6IBS0
Secondary accession number(s): Q9Y3F5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: May 2, 2006
Last modified: November 26, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3