Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycine N-acyltransferase

Gene

GLYAT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial acyltransferase which transfers an acyl group to the N-terminus of glycine and glutamine, although much less efficiently. Can conjugate numerous substrates to form a variety of N-acylglycines, with a preference for benzoyl-CoA over phenylacetyl-CoA as acyl donors. Thereby detoxify xenobiotics, such as benzoic acid or salicylic acid, and endogenous organic acids, such as isovaleric acid.2 Publications

Catalytic activityi

Acyl-CoA + glycine = CoA + N-acylglycine.2 Publications
Benzoyl-CoA + glycine = CoA + hippurate.2 Publications

Kineticsi

  1. KM=57.9 mM for benzoyl-CoA1 Publication
  2. KM=83.7 mM for salicyl-CoA1 Publication
  3. KM=124 mM for isovaleryl-CoA1 Publication
  4. KM=198 mM for octanoyl-CoA1 Publication
  1. Vmax=17.1 µmol/min/mg enzyme with benzoyl-CoA as substrate1 Publication
  2. Vmax=10.1 µmol/min/mg enzyme with salicyl-CoA as substrate1 Publication
  3. Vmax=7.64 µmol/min/mg enzyme with isovaleryl-CoA as substrate1 Publication
  4. Vmax=3.3 µmol/min/mg enzyme with octanoyl-CoA as substrate1 Publication

GO - Molecular functioni

GO - Biological processi

  • acyl-CoA metabolic process Source: ProtInc
  • glycine metabolic process Source: UniProtKB
  • monocarboxylic acid metabolic process Source: UniProtKB
  • response to toxic substance Source: ProtInc
  • small molecule metabolic process Source: Reactome
  • xenobiotic metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Detoxification

Enzyme and pathway databases

BRENDAi2.3.1.13. 2681.
2.3.1.71. 2681.
ReactomeiR-HSA-177128. Conjugation of salicylate with glycine.
R-HSA-177135. Conjugation of benzoate with glycine.
SABIO-RKQ6IB77.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine N-acyltransferase1 Publication (EC:2.3.1.132 Publications)
Alternative name(s):
Acyl-CoA:glycine N-acyltransferase
Short name:
AAc
Aralkyl acyl-CoA N-acyltransferase
Aralkyl acyl-CoA:amino acid N-acyltransferase
Benzoyl-coenzyme A:glycine N-acyltransferase
Glycine N-benzoyltransferase (EC:2.3.1.712 Publications)
HRP-1(CLP)
Gene namesi
Name:GLYAT
Synonyms:ACGNAT, CAT, GAT
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:13734. GLYAT.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • mitochondrial matrix Source: Reactome
  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28748.

Chemistry

DrugBankiDB00145. Glycine.

Polymorphism and mutation databases

BioMutaiGLYAT.
DMDMi311033446.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 296296Glycine N-acyltransferasePRO_0000281869Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei16 – 161N6-acetyllysine; alternateBy similarity
Modified residuei16 – 161N6-succinyllysine; alternateBy similarity
Modified residuei127 – 1271N6-acetyllysine; alternateBy similarity
Modified residuei127 – 1271N6-succinyllysine; alternateBy similarity
Modified residuei141 – 1411N6-acetyllysine; alternateBy similarity
Modified residuei141 – 1411N6-succinyllysine; alternateBy similarity
Modified residuei159 – 1591N6-acetyllysineBy similarity
Modified residuei169 – 1691N6-succinyllysineBy similarity
Modified residuei183 – 1831N6-acetyllysine; alternateBy similarity
Modified residuei183 – 1831N6-succinyllysine; alternateBy similarity
Modified residuei256 – 2561N6-acetyllysine; alternateBy similarity
Modified residuei256 – 2561N6-succinyllysine; alternateBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ6IB77.
PaxDbiQ6IB77.
PRIDEiQ6IB77.

PTM databases

PhosphoSiteiQ6IB77.

Expressioni

Tissue specificityi

Predominantly expressed in liver (at protein level) and kidney. Down-regulated in hepatocellular carcinoma and other liver cancers.1 Publication

Gene expression databases

BgeeiQ6IB77.
CleanExiHS_CAT.
HS_GLYAT.
ExpressionAtlasiQ6IB77. baseline and differential.
GenevisibleiQ6IB77. HS.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
KRTAP10-8P604103EBI-715463,EBI-10171774
NOTCH2NLQ7Z3S93EBI-715463,EBI-945833

Protein-protein interaction databases

BioGridi115543. 5 interactions.
IntActiQ6IB77. 6 interactions.
MINTiMINT-1412441.
STRINGi9606.ENSP00000340200.

Structurei

3D structure databases

ProteinModelPortaliQ6IB77.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycine N-acyltransferase family.Curated

Phylogenomic databases

eggNOGiENOG410IJUM. Eukaryota.
ENOG4111428. LUCA.
GeneTreeiENSGT00390000004997.
HOGENOMiHOG000263599.
HOVERGENiHBG107953.
InParanoidiQ6IB77.
KOiK00628.
OMAiIPRSWNQ.
PhylomeDBiQ6IB77.
TreeFamiTF353258.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR010313. Glycine_N-acyltransferase.
IPR013652. Glycine_N-acyltransferase_C.
IPR015938. Glycine_N-acyltransferase_N.
[Graphical view]
PANTHERiPTHR15298. PTHR15298. 1 hit.
PfamiPF08444. Gly_acyl_tr_C. 1 hit.
PF06021. Gly_acyl_tr_N. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6IB77-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMLPLQGAQM LQMLEKSLRK SLPASLKVYG TVFHINHGNP FNLKAVVDKW
60 70 80 90 100
PDFNTVVVCP QEQDMTDDLD HYTNTYQIYS KDPQNCQEFL GSPELINWKQ
110 120 130 140 150
HLQIQSSQPS LNEAIQNLAA IKSFKVKQTQ RILYMAAETA KELTPFLLKS
160 170 180 190 200
KILSPNGGKP KAINQEMFKL SSMDVTHAHL VNKFWHFGGN ERSQRFIERC
210 220 230 240 250
IQTFPTCCLL GPEGTPVCWD LMDQTGEMRM AGTLPEYRLH GLVTYVIYSH
260 270 280 290
AQKLGKLGFP VYSHVDYSNE AMQKMSYTLQ HVPIPRSWNQ WNCVPL
Length:296
Mass (Da):33,924
Last modified:November 2, 2010 - v3
Checksum:iCB9D63FA48C23FE3
GO
Isoform 2 (identifier: Q6IB77-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     163-163: I → M
     164-296: Missing.

Show »
Length:163
Mass (Da):18,506
Checksum:i07E21AA49DC9D37A
GO

Sequence cautioni

The sequence AAB81453.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti17 – 171S → T.1 Publication
Corresponds to variant rs10896818 [ dbSNP | Ensembl ].
VAR_031294
Natural varianti156 – 1561N → S.4 Publications
Corresponds to variant rs675815 [ dbSNP | Ensembl ].
VAR_031295

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei163 – 1631I → M in isoform 2. 2 PublicationsVSP_024073
Alternative sequencei164 – 296133Missing in isoform 2. 2 PublicationsVSP_024074Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023466 mRNA. Translation: AAB81453.1. Different initiation.
AB013093 mRNA. No translation available.
AP000445 Genomic DNA. No translation available.
BC009785 mRNA. Translation: AAH09785.1.
CR456927 mRNA. Translation: CAG33208.1.
CCDSiCCDS7970.1. [Q6IB77-1]
CCDS7971.1. [Q6IB77-2]
RefSeqiNP_005829.3. NM_005838.3. [Q6IB77-2]
NP_964011.2. NM_201648.2. [Q6IB77-1]
UniGeneiHs.145384.

Genome annotation databases

EnsembliENST00000278400; ENSP00000278400; ENSG00000149124. [Q6IB77-2]
ENST00000344743; ENSP00000340200; ENSG00000149124. [Q6IB77-1]
ENST00000529732; ENSP00000431688; ENSG00000149124. [Q6IB77-1]
ENST00000611865; ENSP00000484592; ENSG00000149124. [Q6IB77-1]
GeneIDi10249.
KEGGihsa:10249.
UCSCiuc001nnb.4. human. [Q6IB77-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023466 mRNA. Translation: AAB81453.1. Different initiation.
AB013093 mRNA. No translation available.
AP000445 Genomic DNA. No translation available.
BC009785 mRNA. Translation: AAH09785.1.
CR456927 mRNA. Translation: CAG33208.1.
CCDSiCCDS7970.1. [Q6IB77-1]
CCDS7971.1. [Q6IB77-2]
RefSeqiNP_005829.3. NM_005838.3. [Q6IB77-2]
NP_964011.2. NM_201648.2. [Q6IB77-1]
UniGeneiHs.145384.

3D structure databases

ProteinModelPortaliQ6IB77.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115543. 5 interactions.
IntActiQ6IB77. 6 interactions.
MINTiMINT-1412441.
STRINGi9606.ENSP00000340200.

Chemistry

DrugBankiDB00145. Glycine.

PTM databases

PhosphoSiteiQ6IB77.

Polymorphism and mutation databases

BioMutaiGLYAT.
DMDMi311033446.

Proteomic databases

EPDiQ6IB77.
PaxDbiQ6IB77.
PRIDEiQ6IB77.

Protocols and materials databases

DNASUi10249.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000278400; ENSP00000278400; ENSG00000149124. [Q6IB77-2]
ENST00000344743; ENSP00000340200; ENSG00000149124. [Q6IB77-1]
ENST00000529732; ENSP00000431688; ENSG00000149124. [Q6IB77-1]
ENST00000611865; ENSP00000484592; ENSG00000149124. [Q6IB77-1]
GeneIDi10249.
KEGGihsa:10249.
UCSCiuc001nnb.4. human. [Q6IB77-1]

Organism-specific databases

CTDi10249.
GeneCardsiGLYAT.
HGNCiHGNC:13734. GLYAT.
MIMi607424. gene.
neXtProtiNX_Q6IB77.
PharmGKBiPA28748.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJUM. Eukaryota.
ENOG4111428. LUCA.
GeneTreeiENSGT00390000004997.
HOGENOMiHOG000263599.
HOVERGENiHBG107953.
InParanoidiQ6IB77.
KOiK00628.
OMAiIPRSWNQ.
PhylomeDBiQ6IB77.
TreeFamiTF353258.

Enzyme and pathway databases

BRENDAi2.3.1.13. 2681.
2.3.1.71. 2681.
ReactomeiR-HSA-177128. Conjugation of salicylate with glycine.
R-HSA-177135. Conjugation of benzoate with glycine.
SABIO-RKQ6IB77.

Miscellaneous databases

GeneWikiiGLYAT.
GenomeRNAii10249.
NextBioi38832.
PROiQ6IB77.
SOURCEiSearch...

Gene expression databases

BgeeiQ6IB77.
CleanExiHS_CAT.
HS_GLYAT.
ExpressionAtlasiQ6IB77. baseline and differential.
GenevisibleiQ6IB77. HS.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR010313. Glycine_N-acyltransferase.
IPR013652. Glycine_N-acyltransferase_C.
IPR015938. Glycine_N-acyltransferase_N.
[Graphical view]
PANTHERiPTHR15298. PTHR15298. 1 hit.
PfamiPF08444. Gly_acyl_tr_C. 1 hit.
PF06021. Gly_acyl_tr_N. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The utilization of alanine, glutamic acid, and serine as amino acid substrates for glycine N-acyltransferase."
    van der Westhuizen F.H., Pretorius P.J., Erasmus E.
    J. Biochem. Mol. Toxicol. 14:102-109(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT SER-156.
  2. "Human HRP-1(CLP)."
    Kishimoto T., Niwa S., Nishikawa K.
    Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT SER-156.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS THR-17 AND SER-156.
    Tissue: Kidney.
  5. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-163, VARIANT SER-156.
  6. "Purification to homogeneity of mitochondrial acyl CoA:glycine N-acyltransferase from human liver."
    Mawal Y.R., Qureshi I.A.
    Biochem. Biophys. Res. Commun. 205:1373-1379(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  7. "Designation of enzyme activity of glycine-N-acyltransferase family genes and depression of glycine-N-acyltransferase in human hepatocellular carcinoma."
    Matsuo M., Terai K., Kameda N., Matsumoto A., Kurokawa Y., Funase Y., Nishikawa K., Sugaya N., Hiruta N., Kishimoto T.
    Biochem. Biophys. Res. Commun. 420:901-906(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiGLYAT_HUMAN
AccessioniPrimary (citable) accession number: Q6IB77
Secondary accession number(s): O14833, Q96QK7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: November 2, 2010
Last modified: March 16, 2016
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.