Q6IB77 (GLYAT_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine N-acyltransferase
EC=2.3.1.13

Alternative name(s):
Acyl-CoA:glycine N-acyltransferase
Short name=AAc
Aralkyl acyl-CoA N-acyltransferase
Aralkyl acyl-CoA:amino acid N-acyltransferase
HRP-1(CLP)

Gene names

Name:	GLYAT
Synonyms:	ACGNAT, CAT, GAT

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitochondrial acyltransferase which transfers the acyl group to the N-terminus of glycine. Can conjugate a multitude of substrates to form a variety of N-acylglycines. Ref.6
Catalytic activity	Acyl-CoA + glycine = CoA + N-acylglycine.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the glycine N-acyltransferase family.
Biophysicochemical properties	Kinetic parameters: KM=57.9 mM for benzoyl-CoA Ref.6 KM=83.7 mM for salicyl-CoA KM=124 mM for isovaleryl-CoA KM=198 mM for octanoyl-CoA Vmax=17.1 µmol/min/mg enzyme with benzoyl-CoA as substrate Vmax=10.1 µmol/min/mg enzyme with salicyl-CoA as substrate Vmax=7.64 µmol/min/mg enzyme with isovaleryl-CoA as substrate Vmax=3.3 µmol/min/mg enzyme with octanoyl-CoA as substrate
Sequence caution	The sequence AAB81453.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Molecular function	Acyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	acyl-CoA metabolic process Traceable author statement. Source: ProtInc response to toxin Traceable author statement. Source: ProtInc xenobiotic metabolic process Traceable author statement. Source: Reactome
Cellular component	mitochondrial matrix Traceable author statement. Source: Reactome
Molecular function	glycine N-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q6IB77-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q6IB77-2)

The sequence of this isoform differs from the canonical sequence as follows:
     163-163: I → M
     164-296: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Glycine N-acyltransferase		PRO_0000281869
Amino acid modifications
	Modified residue	20	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	163	1	I → M in isoform 2.		VSP_024073
	Alternative sequence	164 – 296	133	Missing in isoform 2.		VSP_024074
	Natural variant	17	1	S → T. Ref.4 Corresponds to variant rs10896818 [ dbSNP | Ensembl ].		VAR_031294
	Natural variant	156	1	N → S. Ref.1 Ref.2 Ref.4 Ref.5 Corresponds to variant rs675815 [ dbSNP | Ensembl ].		VAR_031295

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 2, 2010. Version 3. Checksum: CB9D63FA48C23FE3	FASTA	296	33,924
	10 20 30 40 50 60 MMLPLQGAQM LQMLEKSLRK SLPASLKVYG TVFHINHGNP FNLKAVVDKW PDFNTVVVCP 70 80 90 100 110 120 QEQDMTDDLD HYTNTYQIYS KDPQNCQEFL GSPELINWKQ HLQIQSSQPS LNEAIQNLAA 130 140 150 160 170 180 IKSFKVKQTQ RILYMAAETA KELTPFLLKS KILSPNGGKP KAINQEMFKL SSMDVTHAHL 190 200 210 220 230 240 VNKFWHFGGN ERSQRFIERC IQTFPTCCLL GPEGTPVCWD LMDQTGEMRM AGTLPEYRLH 250 260 270 280 290 GLVTYVIYSH AQKLGKLGFP VYSHVDYSNE AMQKMSYTLQ HVPIPRSWNQ WNCVPL
	Isoform 2 [UniParc]. Checksum: 07E21AA49DC9D37A	FASTA	163	18,506
	10 20 30 40 50 60 MMLPLQGAQM LQMLEKSLRK SLPASLKVYG TVFHINHGNP FNLKAVVDKW PDFNTVVVCP 70 80 90 100 110 120 QEQDMTDDLD HYTNTYQIYS KDPQNCQEFL GSPELINWKQ HLQIQSSQPS LNEAIQNLAA 130 140 150 160 IKSFKVKQTQ RILYMAAETA KELTPFLLKS KILSPNGGKP KAM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The utilization of alanine, glutamic acid, and serine as amino acid substrates for glycine N-acyltransferase." van der Westhuizen F.H., Pretorius P.J., Erasmus E. J. Biochem. Mol. Toxicol. 14:102-109(2000) [PubMed: 10630424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT SER-156.
[2]	"Human HRP-1(CLP)." Kishimoto T., Niwa S., Nishikawa K. Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT SER-156.
[3]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed: 16554811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS THR-17 AND SER-156. Tissue: Kidney.
[5]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-163, VARIANT SER-156.
[6]	"Purification to homogeneity of mitochondrial acyl CoA:glycine N-acyltransferase from human liver." Mawal Y.R., Qureshi I.A. Biochem. Biophys. Res. Commun. 205:1373-1379(1994) [PubMed: 7802672] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF023466 mRNA. Translation: AAB81453.1. Different initiation. AB013093 mRNA. No translation available. AP000445 Genomic DNA. No translation available. BC009785 mRNA. Translation: AAH09785.1. CR456927 mRNA. Translation: CAG33208.1.
IPI	IPI00152012. IPI00402759.
RefSeq	NP_005829.3. NM_005838.3. NP_964011.2. NM_201648.2.
UniGene	Hs.145384.
3D structure databases
ProteinModelPortal	Q6IB77.
SMR	Q6IB77. Positions 206-284.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6IB77. 4 interactions.
MINT	MINT-1412441.
STRING	Q6IB77.
PTM databases
PhosphoSite	Q6IB77.
Polymorphism databases
DMDM	311033446.
Proteomic databases
PRIDE	Q6IB77.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000344743; ENSP00000340200; ENSG00000149124.
GeneID	10249.
KEGG	hsa:10249.
UCSC	uc001nnb.1. human. uc001nnc.1. human.
Organism-specific databases
CTD	10249.
GeneCards	GC11M058476.
HGNC	HGNC:13734. GLYAT.
MIM	607424. gene.
neXtProt	NX_Q6IB77.
PharmGKB	PA28748.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG11166.
GeneTree	ENSGT00390000004997.
HOGENOM	HBG506867.
HOVERGEN	HBG107953.
InParanoid	Q6IB77.
OMA	FPVYSHV.
OrthoDB	EOG4ZS93S.
PhylomeDB	Q6IB77.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	Q6IB77.
Bgee	Q6IB77.
CleanEx	HS_CAT. HS_GLYAT.
Genevestigator	Q6IB77.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR010313. Glycine_N-acyltransferase. IPR013652. Glycine_N-acyltransferase_C. IPR015938. Glycine_N-acyltransferase_N. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
KO	K00628.
PANTHER	PTHR15298. Glycine_acyl_tr. 1 hit.
Pfam	PF08444. Gly_acyl_tr_C. 1 hit. PF06021. Gly_acyl_tr_N. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
ProtoNet	Search...
Other
DrugBank	DB00145. Glycine.
NextBio	38832.
SOURCE	Search...

Entry information

Entry name

GLYAT_HUMAN

Accession

Primary (citable) accession number: Q6IB77
Secondary accession number(s): O14833, Q96QK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	November 2, 2010
Last modified:	January 25, 2012
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.