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Protein

E3 ubiquitin-protein ligase HECW2

Gene

Hecw2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination of TP73. Acts to stabilize TP73 and enhance activation of transcription by TP73 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei1546 – 15461Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase HECW2 (EC:6.3.2.-)
Alternative name(s):
HECT, C2 and WW domain-containing protein 2
NEDD4-like E3 ubiquitin-protein ligase 2
Gene namesi
Name:Hecw2
Synonyms:Kiaa1301, Nedl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2685817. Hecw2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15781578E3 ubiquitin-protein ligase HECW2PRO_0000277668Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481PhosphoserineCombined sources
Modified residuei858 – 8581PhosphoserineBy similarity
Modified residuei915 – 9151PhosphoserineBy similarity
Modified residuei1181 – 11811PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6I6G8.
PaxDbiQ6I6G8.
PeptideAtlasiQ6I6G8.
PRIDEiQ6I6G8.

PTM databases

iPTMnetiQ6I6G8.
PhosphoSiteiQ6I6G8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042807.
ExpressionAtlasiQ6I6G8. baseline and differential.
GenevisibleiQ6I6G8. MM.

Interactioni

Subunit structurei

Interacts with TP73.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084942.

Structurei

3D structure databases

ProteinModelPortaliQ6I6G8.
SMRiQ6I6G8. Positions 57-162, 188-285, 815-889, 921-1028, 1174-1575.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini172 – 282111C2PROSITE-ProRule annotationAdd
BLAST
Domaini813 – 84634WW 1PROSITE-ProRule annotationAdd
BLAST
Domaini991 – 102434WW 2PROSITE-ProRule annotationAdd
BLAST
Domaini1243 – 1578336HECTPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni737 – 1074338Interaction with TP73By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili853 – 88028Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation
Contains 2 WW domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG0940. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00760000118966.
HOGENOMiHOG000069940.
HOVERGENiHBG057414.
InParanoidiQ6I6G8.
KOiK12168.
OMAiPTDTRLS.
OrthoDBiEOG091G0A54.
PhylomeDBiQ6I6G8.
TreeFamiTF313938.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR000569. HECT_dom.
IPR032348. HECW_N.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF00632. HECT. 1 hit.
PF16562. HECW_N. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00119. HECTc. 1 hit.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51045. SSF51045. 2 hits.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50237. HECT. 1 hit.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6I6G8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSAREHLL FVRRRNPQMR YTLSPENLQS LAAQNSMPEN MALQRANSDT
60 70 80 90 100
DLVTSESRSS LTASMYEYTL GQAQNLIIFW DIKEEVDPSD WIGLYHIDEN
110 120 130 140 150
SPANFWDSKN RGVTGTQKGQ IVWRIEPGPY FMEPEIKICF KYYHGISGAL
160 170 180 190 200
RATTPCITVK NPAVMMGAEG MEGGASGSLH SRKLVSFTLS DLRAVGLKKG
210 220 230 240 250
MFFNPDPYLK MSIQPGKKSS FPTCAHHGQE RRSTIISNTT NPIWHREKYS
260 270 280 290 300
FFALLTDVLE IEIKDKFAKS RPIIKRFLGK LTIPVQRLLE RQAGDQMLSY
310 320 330 340 350
NLGRRLPADH VSGYLQFKVE VTSSAHEDAS PEAVGTILGV HTVNGDLGSP
360 370 380 390 400
SDEEDMPGSH HDSTICANGP VSEDSVADGT PKHSFRTSST LEIDTEDLIS
410 420 430 440 450
TSSRNSPPRG RQDSLNDYLD AIEHNGPARP GAASSSERSM GASPKLRSSF
460 470 480 490 500
PTDTRLNAML HIDSDEEDHE FQQDLGYPSS LEEEGGLIMC SRASRIDDGS
510 520 530 540 550
LTSQTKPEDD NPVENEDASI HETASLEERL ENLPEVADGS LPSSTAPDEN
560 570 580 590 600
EANLEPQPSA DQGSTELCSS QEVDQPTSGA DAGASDTSGG SRRAASETES
610 620 630 640 650
LDQGSEPSQV SSETEPSDPA RTESVSEAST RPEGESDPEG ADSSCNESVT
660 670 680 690 700
TQLSSVETRC SSLESARFPE TPAFSSQEEE DGACAAEPTS SGPAEGSQES
710 720 730 740 750
VCTPSSLPAV QVPSREEEGS AAEAAALSEQ GELGEVWQRR GSLEGAAAAA
760 770 780 790 800
PAAAATDSQP QEDGDAGDAQ GACEGATAQE EGATGGSQTN GHQPLRSLPS
810 820 830 840 850
VRQDVSRYQR VDEALPPNWE ARIDSHGRIF YVDHVNRTTT WQRPTAPPAP
860 870 880 890 900
QVLQRSNSIQ QMEQLNRRYQ SIRRTMTNER PEENTSAIDG AGEEADFHQA
910 920 930 940 950
SADFRRENVL PHSTSRSRLT LLLQSPPVKF LISPEFFTVL HSNPSAYRMF
960 970 980 990 1000
TNNTCLKHMI TKVRRDTHHF ERYQHNRDLV GFLNMFANKQ LELPRGWEMK
1010 1020 1030 1040 1050
HDHQGKAFFV DHNSRTTTFI DPRLPLQSSR PTSALVHRQH LTRQRSHSAG
1060 1070 1080 1090 1100
EVGEDSRHAG PPVLPRPSST FNTVSRPQYQ DMVPVAYNDK IVAFLRQPNI
1110 1120 1130 1140 1150
LEILQERQPD LARNHSLREK IQFIRTEGTP GLVRLSSDAD LVMLLSLFEE
1160 1170 1180 1190 1200
EIMSYVPPHA LLHPSYCQSP RGSPVSSPQN SPGTQRANAR APAPYKRDFE
1210 1220 1230 1240 1250
AKLRNFYRKL ETKGYGQGPG KLKLIIRRDH LLEDAFNQIM GYSRKDLQRN
1260 1270 1280 1290 1300
KLYVTFVGEE GLDYSGPSRE FFFLVSRELF NPYYGLFEYS ANDTYTVQIS
1310 1320 1330 1340 1350
PMSAFVDNHH EWFRFSGRIL GLALIHQYLL DAFFTRPFYK ALLRILCDLS
1360 1370 1380 1390 1400
DLEYLDEEFH QSLQWMKDND IHDILDLTFT VNEEVFGQIT ERELKPGGAN
1410 1420 1430 1440 1450
IPVTEKNKKE YIERMVKWRI ERGVVQQTES LVRGFYEVVD ARLVSVFDAR
1460 1470 1480 1490 1500
ELELVIAGTA EIDLNDWRNN TEYRGGYHDN HIVIRWFWAA VERFNNEQRL
1510 1520 1530 1540 1550
RLLQFVTGTS SIPYEGFASL RGSNGPRRFC VEKWGKITAL PRAHTCFNRL
1560 1570
DLPPYPSFSM LYEKLLTAVE ETSTFGLE
Length:1,578
Mass (Da):176,235
Last modified:July 19, 2004 - v1
Checksum:i6711C52EA0AF9CBF
GO
Isoform 2 (identifier: Q6I6G8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     295-1578: Missing.

Show »
Length:294
Mass (Da):33,267
Checksum:i7030025F69F569BC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti226 – 2261H → R in BAC31406 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei295 – 15781284Missing in isoform 2. 2 PublicationsVSP_023078Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB182244 mRNA. Translation: BAD23960.1.
AK042922 mRNA. Translation: BAC31406.1.
BC107219 mRNA. Translation: AAI07220.1.
AK129325 mRNA. Translation: BAC98135.1.
CCDSiCCDS14956.1. [Q6I6G8-1]
CCDS14957.1. [Q6I6G8-2]
RefSeqiNP_001001883.1. NM_001001883.3. [Q6I6G8-1]
NP_766243.2. NM_172655.3. [Q6I6G8-2]
XP_006496129.1. XM_006496066.2. [Q6I6G8-1]
XP_006496130.1. XM_006496067.1. [Q6I6G8-1]
XP_006496131.1. XM_006496068.2. [Q6I6G8-1]
UniGeneiMm.132150.
Mm.447527.

Genome annotation databases

EnsembliENSMUST00000087659; ENSMUSP00000084942; ENSMUSG00000042807. [Q6I6G8-1]
ENSMUST00000097741; ENSMUSP00000095348; ENSMUSG00000042807. [Q6I6G8-2]
ENSMUST00000120904; ENSMUSP00000113283; ENSMUSG00000042807. [Q6I6G8-1]
GeneIDi329152.
KEGGimmu:329152.
UCSCiuc007azk.2. mouse. [Q6I6G8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB182244 mRNA. Translation: BAD23960.1.
AK042922 mRNA. Translation: BAC31406.1.
BC107219 mRNA. Translation: AAI07220.1.
AK129325 mRNA. Translation: BAC98135.1.
CCDSiCCDS14956.1. [Q6I6G8-1]
CCDS14957.1. [Q6I6G8-2]
RefSeqiNP_001001883.1. NM_001001883.3. [Q6I6G8-1]
NP_766243.2. NM_172655.3. [Q6I6G8-2]
XP_006496129.1. XM_006496066.2. [Q6I6G8-1]
XP_006496130.1. XM_006496067.1. [Q6I6G8-1]
XP_006496131.1. XM_006496068.2. [Q6I6G8-1]
UniGeneiMm.132150.
Mm.447527.

3D structure databases

ProteinModelPortaliQ6I6G8.
SMRiQ6I6G8. Positions 57-162, 188-285, 815-889, 921-1028, 1174-1575.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000084942.

PTM databases

iPTMnetiQ6I6G8.
PhosphoSiteiQ6I6G8.

Proteomic databases

MaxQBiQ6I6G8.
PaxDbiQ6I6G8.
PeptideAtlasiQ6I6G8.
PRIDEiQ6I6G8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087659; ENSMUSP00000084942; ENSMUSG00000042807. [Q6I6G8-1]
ENSMUST00000097741; ENSMUSP00000095348; ENSMUSG00000042807. [Q6I6G8-2]
ENSMUST00000120904; ENSMUSP00000113283; ENSMUSG00000042807. [Q6I6G8-1]
GeneIDi329152.
KEGGimmu:329152.
UCSCiuc007azk.2. mouse. [Q6I6G8-1]

Organism-specific databases

CTDi57520.
MGIiMGI:2685817. Hecw2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG0940. Eukaryota.
COG5021. LUCA.
GeneTreeiENSGT00760000118966.
HOGENOMiHOG000069940.
HOVERGENiHBG057414.
InParanoidiQ6I6G8.
KOiK12168.
OMAiPTDTRLS.
OrthoDBiEOG091G0A54.
PhylomeDBiQ6I6G8.
TreeFamiTF313938.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

ChiTaRSiHecw2. mouse.
PROiQ6I6G8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042807.
ExpressionAtlasiQ6I6G8. baseline and differential.
GenevisibleiQ6I6G8. MM.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR000008. C2_dom.
IPR000569. HECT_dom.
IPR032348. HECW_N.
IPR001202. WW_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF00632. HECT. 1 hit.
PF16562. HECW_N. 1 hit.
PF00397. WW. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00119. HECTc. 1 hit.
SM00456. WW. 2 hits.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF51045. SSF51045. 2 hits.
SSF56204. SSF56204. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS50237. HECT. 1 hit.
PS01159. WW_DOMAIN_1. 2 hits.
PS50020. WW_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHECW2_MOUSE
AccessioniPrimary (citable) accession number: Q6I6G8
Secondary accession number(s): Q3KNL8, Q6ZPU4, Q8C956
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: July 19, 2004
Last modified: September 7, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.