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Protein

Malate dehydrogenase

Gene

mdh

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36NADUniRule annotation1
Binding sitei87SubstrateUniRule annotation1
Binding sitei93SubstrateUniRule annotation1
Binding sitei100NADUniRule annotation1
Binding sitei125SubstrateUniRule annotation1
Binding sitei156SubstrateUniRule annotation1
Active sitei180Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 17NADUniRule annotation6
Nucleotide bindingi123 – 125NADUniRule annotation3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: UniProtKB-HAMAP
  • malic enzyme activity Source: TIGR

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • carboxylic acid metabolic process Source: InterPro
  • tricarboxylic acid cycle Source: TIGR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciANTHRA:MDH-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:BA_4837, GBAA_4837, BAS4486
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001134241 – 312Malate dehydrogenaseAdd BLAST312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei149PhosphoserineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi198094.BA_4837.

Structurei

Secondary structure

1312
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi15 – 26Combined sources12
Beta strandi31 – 35Combined sources5
Helixi38 – 40Combined sources3
Helixi41 – 58Combined sources18
Beta strandi64 – 68Combined sources5
Helixi70 – 73Combined sources4
Beta strandi77 – 81Combined sources5
Helixi93 – 114Combined sources22
Beta strandi119 – 122Combined sources4
Helixi127 – 138Combined sources12
Helixi142 – 144Combined sources3
Beta strandi145 – 147Combined sources3
Helixi150 – 165Combined sources16
Helixi169 – 171Combined sources3
Beta strandi176 – 178Combined sources3
Helixi181 – 183Combined sources3
Helixi188 – 190Combined sources3
Helixi198 – 200Combined sources3
Helixi204 – 215Combined sources12
Helixi217 – 225Combined sources9
Beta strandi226 – 228Combined sources3
Helixi232 – 246Combined sources15
Beta strandi251 – 261Combined sources11
Helixi262 – 264Combined sources3
Beta strandi266 – 277Combined sources12
Beta strandi280 – 284Combined sources5
Helixi291 – 308Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TL2X-ray1.70A1-312[»]
ProteinModelPortaliQ6HSF4.
SMRiQ6HSF4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiTTNQKIM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6HSF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIKRKKVSV IGAGFTGATT AFLLAQKELA DVVLVDIPQL ENPTKGKALD
60 70 80 90 100
MLEASPVQGF DANIIGTSDY ADTADSDVVV ITAGIARKPG MSRDDLVATN
110 120 130 140 150
SKIMKSITRD IAKHSPNAII VVLTNPVDAM TYSVFKEAGF PKERVIGQSG
160 170 180 190 200
VLDTARFRTF IAQELNLSVK DITGFVLGGH GDDMVPLVRY SYAGGIPLET
210 220 230 240 250
LIPKERLEAI VERTRKGGGE IVGLLGNGSA YYAPAASLVE MTEAILKDQR
260 270 280 290 300
RVLPAIAYLE GEYGYSDLYL GVPVILGGNG IEKIIELELL ADEKEALDRS
310
VESVRNVMKV LV
Length:312
Mass (Da):33,498
Last modified:July 19, 2004 - v1
Checksum:i615A61C61CA217FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28526.1.
AE017334 Genomic DNA. Translation: AAT33956.1.
AE017225 Genomic DNA. Translation: AAT56784.1.
RefSeqiNP_847040.1. NC_003997.3.
WP_000153232.1. NZ_LHUO01000006.1.
YP_030734.1. NC_005945.1.

Genome annotation databases

EnsemblBacteriaiAAP28526; AAP28526; BA_4837.
AAT33956; AAT33956; GBAA_4837.
AAT56784; AAT56784; BAS4486.
GeneIDi1083988.
2851560.
KEGGiban:BA_4837.
bar:GBAA_4837.
bat:BAS4486.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA. Translation: AAP28526.1.
AE017334 Genomic DNA. Translation: AAT33956.1.
AE017225 Genomic DNA. Translation: AAT56784.1.
RefSeqiNP_847040.1. NC_003997.3.
WP_000153232.1. NZ_LHUO01000006.1.
YP_030734.1. NC_005945.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TL2X-ray1.70A1-312[»]
ProteinModelPortaliQ6HSF4.
SMRiQ6HSF4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198094.BA_4837.

Protocols and materials databases

DNASUi1083988.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP28526; AAP28526; BA_4837.
AAT33956; AAT33956; GBAA_4837.
AAT56784; AAT56784; BAS4486.
GeneIDi1083988.
2851560.
KEGGiban:BA_4837.
bar:GBAA_4837.
bat:BAS4486.

Phylogenomic databases

eggNOGiENOG4105C80. Bacteria.
COG0039. LUCA.
HOGENOMiHOG000213794.
KOiK00024.
OMAiTTNQKIM.

Enzyme and pathway databases

BioCyciANTHRA:MDH-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00487. Malate_dehydrog_3. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_BACAN
AccessioniPrimary (citable) accession number: Q6HSF4
Secondary accession number(s): Q6KLP9, Q81KZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.