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Q6HSF4

- MDH_BACAN

UniProt

Q6HSF4 - MDH_BACAN

Protein

Malate dehydrogenase

Gene

mdh

Organism
Bacillus anthracis
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361NADUniRule annotation
    Binding sitei87 – 871SubstrateUniRule annotation
    Binding sitei93 – 931SubstrateUniRule annotation
    Binding sitei100 – 1001NADUniRule annotation
    Binding sitei125 – 1251SubstrateUniRule annotation
    Binding sitei156 – 1561SubstrateUniRule annotation
    Active sitei180 – 1801Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 176NADUniRule annotation
    Nucleotide bindingi123 – 1253NADUniRule annotation

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciANTHRA:MDH-MONOMER.
    BANT260799:GJAJ-4544-MONOMER.
    BANT261594:GJ7F-4699-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:BA_4837, GBAA_4837, BAS4486
    OrganismiBacillus anthracis
    Taxonomic identifieri1392 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000000427: Chromosome, UP000000594: Chromosome, UP000005639: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 312312Malate dehydrogenasePRO_0000113424Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei149 – 1491PhosphoserineUniRule annotation

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi198094.BA_4837.

    Structurei

    Secondary structure

    1
    312
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 115
    Helixi15 – 2612
    Beta strandi31 – 355
    Helixi38 – 403
    Helixi41 – 5818
    Beta strandi64 – 685
    Helixi70 – 734
    Beta strandi77 – 815
    Helixi93 – 11422
    Beta strandi119 – 1224
    Helixi127 – 13812
    Helixi142 – 1443
    Beta strandi145 – 1473
    Helixi150 – 16516
    Helixi169 – 1713
    Beta strandi176 – 1783
    Helixi181 – 1833
    Helixi188 – 1903
    Helixi198 – 2003
    Helixi204 – 21512
    Helixi217 – 2259
    Beta strandi226 – 2283
    Helixi232 – 24615
    Beta strandi251 – 26111
    Helixi262 – 2643
    Beta strandi266 – 27712
    Beta strandi280 – 2845
    Helixi291 – 30818

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TL2X-ray1.70A1-312[»]
    ProteinModelPortaliQ6HSF4.
    SMRiQ6HSF4. Positions 5-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000213794.
    KOiK00024.
    OMAiFVINVAN.
    OrthoDBiEOG6091FG.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_00487. Malate_dehydrog_3.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01763. MalateDH_bact. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q6HSF4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTIKRKKVSV IGAGFTGATT AFLLAQKELA DVVLVDIPQL ENPTKGKALD    50
    MLEASPVQGF DANIIGTSDY ADTADSDVVV ITAGIARKPG MSRDDLVATN 100
    SKIMKSITRD IAKHSPNAII VVLTNPVDAM TYSVFKEAGF PKERVIGQSG 150
    VLDTARFRTF IAQELNLSVK DITGFVLGGH GDDMVPLVRY SYAGGIPLET 200
    LIPKERLEAI VERTRKGGGE IVGLLGNGSA YYAPAASLVE MTEAILKDQR 250
    RVLPAIAYLE GEYGYSDLYL GVPVILGGNG IEKIIELELL ADEKEALDRS 300
    VESVRNVMKV LV 312
    Length:312
    Mass (Da):33,498
    Last modified:July 19, 2004 - v1
    Checksum:i615A61C61CA217FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP28526.1.
    AE017334 Genomic DNA. Translation: AAT33956.1.
    AE017225 Genomic DNA. Translation: AAT56784.1.
    RefSeqiNP_847040.1. NC_003997.3.
    YP_021481.1. NC_007530.2.
    YP_030734.1. NC_005945.1.

    Genome annotation databases

    EnsemblBacteriaiAAP28526; AAP28526; BA_4837.
    AAT33956; AAT33956; GBAA_4837.
    AAT56784; AAT56784; BAS4486.
    GeneIDi1083988.
    2819626.
    2851560.
    KEGGiban:BA_4837.
    bar:GBAA_4837.
    bat:BAS4486.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016879 Genomic DNA. Translation: AAP28526.1 .
    AE017334 Genomic DNA. Translation: AAT33956.1 .
    AE017225 Genomic DNA. Translation: AAT56784.1 .
    RefSeqi NP_847040.1. NC_003997.3.
    YP_021481.1. NC_007530.2.
    YP_030734.1. NC_005945.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TL2 X-ray 1.70 A 1-312 [» ]
    ProteinModelPortali Q6HSF4.
    SMRi Q6HSF4. Positions 5-309.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 198094.BA_4837.

    Protocols and materials databases

    DNASUi 1083988.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP28526 ; AAP28526 ; BA_4837 .
    AAT33956 ; AAT33956 ; GBAA_4837 .
    AAT56784 ; AAT56784 ; BAS4486 .
    GeneIDi 1083988.
    2819626.
    2851560.
    KEGGi ban:BA_4837.
    bar:GBAA_4837.
    bat:BAS4486.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000213794.
    KOi K00024.
    OMAi FVINVAN.
    OrthoDBi EOG6091FG.

    Enzyme and pathway databases

    BioCyci ANTHRA:MDH-MONOMER.
    BANT260799:GJAJ-4544-MONOMER.
    BANT261594:GJ7F-4699-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_00487. Malate_dehydrog_3.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01763. MalateDH_bact. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Bacillus anthracis Ames and comparison to closely related bacteria."
      Read T.D., Peterson S.N., Tourasse N.J., Baillie L.W., Paulsen I.T., Nelson K.E., Tettelin H., Fouts D.E., Eisen J.A., Gill S.R., Holtzapple E.K., Okstad O.A., Helgason E., Rilstone J., Wu M., Kolonay J.F., Beanan M.J., Dodson R.J.
      , Brinkac L.M., Gwinn M.L., DeBoy R.T., Madpu R., Daugherty S.C., Durkin A.S., Haft D.H., Nelson W.C., Peterson J.D., Pop M., Khouri H.M., Radune D., Benton J.L., Mahamoud Y., Jiang L., Hance I.R., Weidman J.F., Berry K.J., Plaut R.D., Wolf A.M., Watkins K.L., Nierman W.C., Hazen A., Cline R.T., Redmond C., Thwaite J.E., White O., Salzberg S.L., Thomason B., Friedlander A.M., Koehler T.M., Hanna P.C., Kolstoe A.-B., Fraser C.M.
      Nature 423:81-86(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames / isolate Porton.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Ames ancestor.
    3. "Complete genome sequence of Bacillus anthracis Sterne."
      Brettin T.S., Bruce D., Challacombe J.F., Gilna P., Han C., Hill K., Hitchcock P., Jackson P., Keim P., Longmire J., Lucas S., Okinaka R., Richardson P., Rubin E., Tice H.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sterne.

    Entry informationi

    Entry nameiMDH_BACAN
    AccessioniPrimary (citable) accession number: Q6HSF4
    Secondary accession number(s): Q6KLP9, Q81KZ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3