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Protein

Malate dehydrogenase

Gene

mdh

Organism
Bacillus anthracis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36NADUniRule annotation1
Binding sitei87SubstrateUniRule annotation1
Binding sitei93SubstrateUniRule annotation1
Binding sitei100NADUniRule annotation1
Binding sitei125SubstrateUniRule annotation1
Binding sitei156SubstrateUniRule annotation1
Active sitei180Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 17NADUniRule annotation6
Nucleotide bindingi123 – 125NADUniRule annotation3

GO - Molecular functioni

  • L-malate dehydrogenase activity Source: UniProtKB-EC
  • malic enzyme activity Source: TIGR

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • tricarboxylic acid cycle Source: TIGR

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:BA_4837, GBAA_4837, BAS4486
OrganismiBacillus anthracis
Taxonomic identifieri1392 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000000594 Componenti: Chromosome
  • UP000000427 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001134241 – 312Malate dehydrogenaseAdd BLAST312

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei149PhosphoserineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi260799.BAS4486

Structurei

Secondary structure

1312
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi15 – 26Combined sources12
Beta strandi31 – 35Combined sources5
Helixi38 – 40Combined sources3
Helixi41 – 58Combined sources18
Beta strandi64 – 68Combined sources5
Helixi70 – 73Combined sources4
Beta strandi77 – 81Combined sources5
Helixi93 – 114Combined sources22
Beta strandi119 – 122Combined sources4
Helixi127 – 138Combined sources12
Helixi142 – 144Combined sources3
Beta strandi145 – 147Combined sources3
Helixi150 – 165Combined sources16
Helixi169 – 171Combined sources3
Beta strandi176 – 178Combined sources3
Helixi181 – 183Combined sources3
Helixi188 – 190Combined sources3
Helixi198 – 200Combined sources3
Helixi204 – 215Combined sources12
Helixi217 – 225Combined sources9
Beta strandi226 – 228Combined sources3
Helixi232 – 246Combined sources15
Beta strandi251 – 261Combined sources11
Helixi262 – 264Combined sources3
Beta strandi266 – 277Combined sources12
Beta strandi280 – 284Combined sources5
Helixi291 – 308Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TL2X-ray1.70A1-312[»]
ProteinModelPortaliQ6HSF4
SMRiQ6HSF4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA
HOGENOMiHOG000213794
KOiK00024
OMAiRQPGQTR

Family and domain databases

CDDicd01339 LDH-like_MDH, 1 hit
Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00487 Malate_dehydrog_3, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR011275 Malate_DH_type3
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01763 MalateDH_bact, 1 hit

Sequencei

Sequence statusi: Complete.

Q6HSF4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIKRKKVSV IGAGFTGATT AFLLAQKELA DVVLVDIPQL ENPTKGKALD
60 70 80 90 100
MLEASPVQGF DANIIGTSDY ADTADSDVVV ITAGIARKPG MSRDDLVATN
110 120 130 140 150
SKIMKSITRD IAKHSPNAII VVLTNPVDAM TYSVFKEAGF PKERVIGQSG
160 170 180 190 200
VLDTARFRTF IAQELNLSVK DITGFVLGGH GDDMVPLVRY SYAGGIPLET
210 220 230 240 250
LIPKERLEAI VERTRKGGGE IVGLLGNGSA YYAPAASLVE MTEAILKDQR
260 270 280 290 300
RVLPAIAYLE GEYGYSDLYL GVPVILGGNG IEKIIELELL ADEKEALDRS
310
VESVRNVMKV LV
Length:312
Mass (Da):33,498
Last modified:July 19, 2004 - v1
Checksum:i615A61C61CA217FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016879 Genomic DNA Translation: AAP28526.1
AE017334 Genomic DNA Translation: AAT33956.1
AE017225 Genomic DNA Translation: AAT56784.1
RefSeqiNP_847040.1, NC_003997.3
WP_000153232.1, NZ_NVMT01000026.1
YP_030734.1, NC_005945.1

Genome annotation databases

EnsemblBacteriaiAAP28526; AAP28526; BA_4837
AAT33956; AAT33956; GBAA_4837
AAT56784; AAT56784; BAS4486
GeneIDi1083988
2851560
31627966
KEGGiban:BA_4837
bar:GBAA_4837
bat:BAS4486
PATRICifig|198094.11.peg.4798

Similar proteinsi

Entry informationi

Entry nameiMDH_BACAN
AccessioniPrimary (citable) accession number: Q6HSF4
Secondary accession number(s): Q6KLP9, Q81KZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 19, 2004
Last modified: April 25, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health