Reviewed, UniProtKB/Swiss-Prot Q6HNZ7 (SYP2_BACHK)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase 2
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase 2
      Short name=ProRS 2

Gene names

Name:	proS2
Ordered Locus Names:	BT9727_0373

Organism

Bacillus thuringiensis subsp. konkukian [Complete proteome] [HAMAP]

Taxonomic identifier

180856 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Prolyl-tRNA synthetase 2 HAMAP MF_01571		PRO_0000249122

Sequences

	Sequence		Length	Mass (Da)
	Q6HNZ7-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 5F168CFF20416E21	FASTA	476	54,639
	10 20 30 40 50 60 MAKEQVQAIT KMEEDFAQWY TDIVKKAELV DYSSVKGCMI LRPYGYALWE NMQKVMDEKL 70 80 90 100 110 120 KATGHENVYM PMFIPESLLQ KEKDHVEGFA PEVAWVTHGG DEKLAERLCV RPTSETLFCE 130 140 150 160 170 180 HFSKIVQSYN DLPKLYNQWC SVVRWEKTTR PFLRTTEFLW QEGHTIHETA EESQAETLNI 190 200 210 220 230 240 LNLYASFCED YLAIPVIKGQ KTEKEKFAGA KATYTIESLM HDGKALQTGT SHNFGTNFSE 250 260 270 280 290 300 AFDIKFLDRN GKWQYVHQTS WGVSTRMIGG LIMVHSDNNG LVMPPKVAPV QVVIVPIAQH 310 320 330 340 350 360 KEGVLAKAIE LQGHIQKVAR VKIDASNKTP GWKFNEYEMK GIPIRLEVGP KDIEKNQVVL 370 380 390 400 410 420 VRRDTKEKEF ISMDQLEERI PALLEEIHNS LFNKAKVFRD ENTYSVTSFE EMKKVADEKQ 430 440 450 460 470 GFIKAMWCGE VACEEKLKEE VGVSSRCMPF EQEHLADECV CCGKEAKQMV YWGKAY

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 97-27.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017355 Genomic DNA. Translation: AAT61247.1.
RefSeq	YP_034723.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2857611.
GenomeReviews	Gene locus BT9727_0373 in contig AE017355_GR.
KEGG	btk:BT9727_0373.
NMPDR	fig|281309.1.peg.363.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6HNZ7.
OMA	CIEAMMQ.
Enzyme and pathway databases
BioCyc	BTHU281309:BT9727_0373-MON.
Family and domain databases
HAMAP	MF_01571. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR004499. Pro-tRNA-synth_IIa_pro-type. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP2_BACHK

Accession

Primary (citable) accession number: Q6HNZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)