Q6HM28 (HEMH2_BACHK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase 2
EC=4.99.1.1

Alternative name(s):
Heme synthase 2
Protoheme ferro-lyase 2

Gene names

Name:	hemH2
Ordered Locus Names:	BT9727_1056

Organism

Bacillus thuringiensis subsp. konkukian (strain 97-27) [Complete proteome] [HAMAP]

Taxonomic identifier

281309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Ferrochelatase 2 HAMAP MF_00323		PRO_0000175112
Sites
	Metal binding	181	1	Iron By similarity
	Metal binding	262	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6HM28 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: CB3C6D21AFB356F6	FASTA	319	36,320
	10 20 30 40 50 60 MKKKKIGLLV MAYGTPDSLD EVEAYYTHIR HGRKPSEEAL QDLIGRYKAI GGISPLAKIT 70 80 90 100 110 120 KEQAHKLTDS MNNMFTEYEF NCYLGLKHTA PFIEDAVEEM KRDGIEQAIS IVLAPHYSTF 130 140 150 160 170 180 SIKAYNERAI RLSEEIGGPV IEPIDQWYDE PKFISYWADQ IKETFTKIED NEKAVVIFSA 190 200 210 220 230 240 HSLPEKIIAA GDPYVEQLQY TADLIAAAAN IQNYTIGWQS AGNTSDSWIG PDVQDLTRDL 250 260 270 280 290 300 FEEHRYESFI YCPVGFVAEH LEVLYDNDYE CKVVTDELNA AYFRPTMPNA QSTFIDCLAT 310 IVSRKMKEIV DKELILNNN

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 97-27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017355 Genomic DNA. Translation: AAT62227.1.
RefSeq	YP_035393.1. NC_005957.1.
3D structure databases
ProteinModelPortal	Q6HM28.
SMR	Q6HM28. Positions 3-310.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000075597; EBBACP00000073683; EBBACG00000075588.
GeneID	2855309.
GenomeReviews	Gene locus BT9727_1056 in contig AE017355_GR.
KEGG	btk:BT9727_1056.
NMPDR	fig|281309.1.peg.1033.
PATRIC	18982759. VBIBacThu119411_1110.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000002085.
HOGENOM	HBG697135.
OMA	LEPDIVD.
ProtClustDB	PRK12435.
Enzyme and pathway databases
BioCyc	BTHU281309:BT9727_1056-MONOMER.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH2_BACHK

Accession

Primary (citable) accession number: Q6HM28

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program