Reviewed, UniProtKB/Swiss-Prot Q6HLX9 (PRPE_BACHK)

Names and origin

Protein names

Recommended name:
    Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical]
    EC=3.6.1.17
Alternative name(s):
    Ap4A hydrolase
    Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase
      Short name=Diadenosine tetraphosphatase

Gene names

Name:	prpE
Ordered Locus Names:	BT9727_1105

Organism

Bacillus thuringiensis subsp. konkukian [Complete proteome] [HAMAP]

Taxonomic identifier

180856 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Asymmetrically hydrolyzes Ap4p to yield AMP and ATP By similarity. HAMAP MF_01443
Catalytic activity	P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP. HAMAP MF_01443
Cofactor	Nickel By similarity. HAMAP MF_01443
Sequence similarities	Belongs to the prpE family.

Ontologies

Keywords
Ligand	GTP-binding Nickel Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity Inferred from electronic annotation. Source: HAMAP nickel ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Bis(5'-nucleosyl)-tetraphosphatase prpE [asymmetrical] HAMAP MF_01443		PRO_0000297702

Sequences

	Sequence		Length	Mass (Da)
	Q6HLX9-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 4D0936F73DFA4FA1	FASTA	246	28,227
	10 20 30 40 50 60 MKYDIIGDIH GCLQEFQNLT EKLGYNWSSG LPIHPDQRKL AFVGDITDRG PHSLRMIEIV 70 80 90 100 110 120 WELVIHKKVA YYAPGNHCNK LYRFFLGRNV TIAHGLETTV AEYEALPSHK QNMIKEKFIT 130 140 150 160 170 180 LYEQSPLYHV LDEKRLLVCH AGIRQDYIGR QDKKVQTFVL YGDITGEKHA DGSPVRRDWA 190 200 210 220 230 240 KEYKGTTWIV YGHTPVKEPR FVNHTVNIDT GAVFGGKLTG LRYPEMETVS VPSSLPFVPE KFRPIS

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 97-27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017355 Genomic DNA. Translation: AAT59336.1.
RefSeq	YP_035442.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2854146.
GenomeReviews	Gene locus BT9727_1105 in contig AE017355_GR.
KEGG	btk:BT9727_1105.
NMPDR	fig|281309.1.peg.1082.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG724457.
OMA	HCNKLYR.
Enzyme and pathway databases
BioCyc	BTHU281309:BT9727_1105-MONOMER.
Family and domain databases
HAMAP	MF_01443. PrpE. [Tree]
InterPro	IPR004843. M-pesterase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRPE_BACHK

Accession

Primary (citable) accession number: Q6HLX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	July 19, 2004
Last modified:	January 19, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)