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Q6HLK3 (QUEF_BACHK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13
Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase
Gene names
Name:queF
Ordered Locus Names:BT9727_1233
OrganismBacillus thuringiensis subsp. konkukian (strain 97-27) [Complete proteome] [HAMAP]
Taxonomic identifier281309 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length165 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818

Catalytic activity

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818

Subcellular location

Cytoplasm Probable HAMAP MF_00818.

Sequence similarities

Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processqueuosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionoxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor

Inferred from electronic annotation. Source: InterPro

preQ1 synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 165165NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818
PRO_0000162958

Sequences

Sequence LengthMass (Da)Tools
Q6HLK3 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: E739117C7A7FF755

FASTA16519,527
        10         20         30         40         50         60 
MAGRLDEDLK DVTLLGNQNT KYLFEYSPKI LEVFDNNHPN RDYFVKFNCP EFTSLCPKTG 

        70         80         90        100        110        120 
QPDFATIYIS YIPEQRMVES KSLKLYLFSF RNHGDFHEDC MNVIMNDLIK LMDPRYIEVW 

       130        140        150        160 
GKFTPRGGIS IDPYCNYGRP GTKYEQMADY RMMNHDLYPE TIDNR

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017355 Genomic DNA. Translation: AAT61963.1.
RefSeqYP_035568.1. NC_005957.1.

3D structure databases

ProteinModelPortalQ6HLK3.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000074024; EBBACP00000072110; EBBACG00000074015.
GeneID2855414.
GenomeReviewsGene locus BT9727_1233 in contig AE017355_GR.
KEGGbtk:BT9727_1233.
NMPDRfig|281309.1.peg.1208.
PATRIC18983135. VBIBacThu119411_1297.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000002144.
HOGENOMHBG294103.
OMACINTILL.
ProtClustDBPRK13258.

Enzyme and pathway databases

BioCycBTHU281309:BT9727_1233-MONOMER.

Family and domain databases

HAMAPMF_00818. QueF_type1.
[Tree]
InterProIPR016856. CN_OxRdtase_NADPH-dep_QueF.
IPR020602. GTP_CycHdrlase_I/CN_OxRdtase.
[Graphical view]
KOK09457.
PfamPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
PIRSFPIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMsTIGR03139. QueF-II. 1 hit.
ProtoNetSearch...

Entry information

Entry nameQUEF_BACHK
AccessionPrimary (citable) accession number: Q6HLK3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: July 19, 2004
Last modified: January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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