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Q6HLF2 (LEU3_BACHK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydrogenase
EC=1.1.1.85
Alternative name(s):
3-IPM-DH
Beta-IPM dehydrogenase
Short name=IMDH
Gene names
Name:leuB
Ordered Locus Names:BT9727_1285
OrganismBacillus thuringiensis subsp. konkukian (strain 97-27) [Complete proteome] [HAMAP]
Taxonomic identifier281309 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033

Subunit structure

Homodimer By similarity. HAMAP MF_01033

Subcellular location

Cytoplasm By similarity HAMAP MF_01033.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3543543-isopropylmalate dehydrogenase HAMAP MF_01033
PRO_0000083643

Regions

Nucleotide binding76 – 8712NAD By similarity
Nucleotide binding273 – 28513NAD By similarity

Sites

Metal binding2151Magnesium or manganese By similarity
Metal binding2391Magnesium or manganese By similarity
Metal binding2431Magnesium or manganese By similarity
Binding site941Substrate By similarity
Binding site1041Substrate By similarity
Binding site1301Substrate By similarity
Binding site2151Substrate By similarity
Site1371Important for catalysis By similarity
Site1831Important for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6HLF2 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: D7F4738258A3641F

FASTA35438,274
        10         20         30         40         50         60 
MEKRIVCLAG DGVGPEVMES AKGVLHMVER LYGHHFHLQD EHFGGVAIDL TGQPLPQRTL 

        70         80         90        100        110        120 
AACLASDAVL LGAVGGPRWD GAKERPEKGL LALRKGLGVF ANVRPVTVES ATAHLSPLKK 

       130        140        150        160        170        180 
ADEIDFVVVR ELTGGIYFSY PKKRTDEVAT DTLTYHRHEI ERIVSYAFQL ASKRKKKVTS 

       190        200        210        220        230        240 
IDKANVLESS KLWRTVTEEV ALRYPDVELE HILVDAAAME LIRNPGRFDV IVTENLFGDI 

       250        260        270        280        290        300 
LSDEASVLAG SLGMLPSASH AEKGPSLYEP IHGSAPDIAG KNKANPIAMM RSVAMMLGQS 

       310        320        330        340        350 
FGLTREGCAI EEAISAVLKS GKCTADIGGA ETTTSFTKAV MQEMEEQALV GRGR

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017355 Genomic DNA. Translation: AAT59415.1.
RefSeqYP_035619.1. NC_005957.1.

3D structure databases

ProteinModelPortalQ6HLF2.
SMRQ6HLF2. Positions 1-344.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000072437; EBBACP00000070523; EBBACG00000072428.
GeneID2855825.
GenomeReviewsGene locus BT9727_1285 in contig AE017355_GR.
KEGGbtk:BT9727_1285.
NMPDRfig|281309.1.peg.1259.
PATRIC18983249. VBIBacThu119411_1354.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000000661.
HOGENOMHBG518924.
OMALDKNNKG.
ProtClustDBPRK00772.

Enzyme and pathway databases

BioCycBTHU281309:BT9727_1285-MONOMER.

Family and domain databases

HAMAPMF_01033. LeuB_type1.
[Tree]
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KOK00052.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF13. IPMDH. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. LeuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU3_BACHK
AccessionPrimary (citable) accession number: Q6HLF2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 19, 2004
Last modified: January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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