Q6HKA1 (ILVC2_BACHK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase 2
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase 2
Alpha-keto-beta-hydroxylacil reductoisomerase 2

Gene names

Name:	ilvC2
Ordered Locus Names:	BT9727_1693

Organism

Bacillus thuringiensis subsp. konkukian (strain 97-27) [Complete proteome] [HAMAP]

Taxonomic identifier

281309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Ketol-acid reductoisomerase 2 HAMAP MF_00435		PRO_0000226160
Sites
	Active site	106	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6HKA1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 8F53BEEB4FDF24E3	FASTA	335	36,917
	10 20 30 40 50 60 MKTYYEQDAN VGLLQGKTVA VIGYGSQGHA QAQNLRDSGV EVVVGVRPGK SFEVAKADGF 70 80 90 100 110 120 KVMSVSEAVR TAQVVQMLLP DEQQAHVYKA EVEENLREGQ MLLFSHGFNI HFGQINPPSY 130 140 150 160 170 180 VDVAMVAPKS PGHLVRRVFQ EGNGVPALVA VHQDATGTAL HVALAYAKGV GCTRAGVIET 190 200 210 220 230 240 TFQEETETDL FGEQAVLCGG VTALVKAGFE TLTEGGYRPE IAYFECLHEL KLIVDLMYEG 250 260 270 280 290 300 GLTNMRHSIS DTAEFGDYVT GSRIVTDETK KEMKRVLTEI QQGEFAKKWI LENQAGRPTY 310 320 330 NAMKKAEQNH QLEKVGEELR EMMSWIHAPK ELVKK

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 97-27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017355 Genomic DNA. Translation: AAT59605.1.
RefSeq	YP_036025.1. NC_005957.1.
3D structure databases
ProteinModelPortal	Q6HKA1.
SMR	Q6HKA1. Positions 1-326.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000076142; EBBACP00000074228; EBBACG00000076133.
GeneID	2856800.
GenomeReviews	Gene locus BT9727_1693 in contig AE017355_GR.
KEGG	btk:BT9727_1693.
NMPDR	fig|281309.1.peg.1665.
PATRIC	18984108. VBIBacThu119411_1783.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000000943.
HOGENOM	HBG286525.
OMA	RPGKSYE.
ProtClustDB	PRK13403.
Enzyme and pathway databases
BioCyc	BTHU281309:BT9727_1693-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC2_BACHK

Accession

Primary (citable) accession number: Q6HKA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program