Q6HJW8 (NADE_BACHK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BT9727_1826

Organism

Bacillus thuringiensis subsp. konkukian (strain 97-27) [Complete proteome] [HAMAP]

Taxonomic identifier

281309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077536
Regions
	Nucleotide binding	45 – 52	8	ATP By similarity
Sites
	Active site	47	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6HJW8 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: B581BB92E8003798	FASTA	272	30,117
	10 20 30 40 50 60 MTLQEQIMKA LHVQPVIDLK AEIRKRVDFL KDYVKKTGAK GFVLGISGGQ DSTLAGRLAQ 70 80 90 100 110 120 LAVEEIRNEG GNATFIAVRL PYKVQKDEDD AQLALQFIQA DQSVAFDIAS TVDAFSNQYE 130 140 150 160 170 180 NLLDESLTDF NKGNVKARIR MVTQYAIGGQ KGLLVIGTDH AAEAVTGFFT KFGDGGADLL 190 200 210 220 230 240 PLTGLTKRQG RALLQELGAD ERLYLKMPTA DLLDEKPGQA DETELGITYD QLDDYLEGKT 250 260 270 VPADVAEKIE KRYTVSEHKR QVPASMFDDW WK

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 97-27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017355 Genomic DNA. Translation: AAT63415.1.
RefSeq	YP_036158.1. NC_005957.1.
3D structure databases
HSSP	HSSP built from PDB template 1IH8 based on UniProtKB P08164.
ProteinModelPortal	Q6HJW8.
SMR	Q6HJW8. Positions 1-272.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000074802; EBBACP00000072888; EBBACG00000074793.
GeneID	2853830.
GenomeReviews	Gene locus BT9727_1826 in contig AE017355_GR.
KEGG	btk:BT9727_1826.
NMPDR	fig|281309.1.peg.1798.
PATRIC	18984389. VBIBacThu119411_1923.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001308.
HOGENOM	HBG351567.
OMA	GLNKRRV.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	BTHU281309:BT9727_1826-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BACHK

Accession

Primary (citable) accession number: Q6HJW8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program