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Q6HJF2

- SYI2_BACHK

UniProt

Q6HJF2 - SYI2_BACHK

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Protein

Isoleucine--tRNA ligase 2

Gene
ileS2, BT9727_1995
Organism
Bacillus thuringiensis subsp. konkukian (strain 97-27)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei593 – 5931ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBTHU281309:GJID-2078-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 2 (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase 2
Short name:
IleRS 2
Gene namesi
Name:ileS2
Ordered Locus Names:BT9727_1995
OrganismiBacillus thuringiensis subsp. konkukian (strain 97-27)
Taxonomic identifieri281309 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000001301: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10331033Isoleucine--tRNA ligase 2UniRule annotationPRO_0000098519Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi281309.BT9727_1995.

Structurei

3D structure databases

ProteinModelPortaliQ6HJF2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionUniRule annotationAdd
BLAST
Motifi590 – 5945"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiSEGMEPN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6HJF2-1 [UniParc]FASTAAdd to Basket

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MKKVDVKESA VGRETRIRKQ WNEQSIFEQS IQNREGAQSF VFYEGPPTAN     50
GLPHVGHALG RTIKDVVARY KTMAGYKVLR KAGWDTHGLP VELGVEKQLG 100
ISGKHEIEEY GIEPFTKKCK ESVFTYEKQW REFTESIGYW VDMDDPYVTL 150
ENPYIESVWH ILGTIHEKGL LYKGHRVSPY CPSCQTSLSS HEVAQGYKTV 200
KDLSATVKFK VKDSENEYFL GWTTTPWTLP ANVALAVHPN MEYVKAKQEG 250
HVYIVAKERV QDVLKENYEV LSVHKGEELL NTSYTAPFPM KEVTNGYRVI 300
GADFVTADSG TGLVHIAPAY GEDDYRVVQS EGLSFLHVVD EKGEYTEVVP 350
FLKGKFVKDC DVDIVRYLAK EGLLYHKEKY EHSYPHCWRC DSPLLYYAGE 400
SWLIRTTAIK DTFLQNNDTV TWYPDHMKHG RFGKFLENMV DWNISRNRYW 450
GTPLNVWECE SCDHQFAPKS IADLRKHSTK ETPEDLELHK PYVDEVQVSC 500
EKCGSAMNRT PEVIDVWFDS GSMPFAQYHY PFENKELFEE QFPADVIAEG 550
IDQTRGWFYS LLAVSALYTG KVPYKRVLSL GHVLDEEGQK MSKSKGNALD 600
PVDLVGKFGA DALRWALLVD SAPWNAKRFS ERTVLEAKSK FVDTLVNVYS 650
FYVLYANLDE YNPNETYDVK RTKLDEWVLS RLHSTTKKVR TALDDYQFTN 700
AAREIAALVD EVSNWYVRRS RNRFWESGMN AEKAAAYETL HDVLVTISKL 750
IAPFTPFVAE DIHLNLTGSS VHLEDYPVVN ESLLQPKLEA EMDAVLQVVE 800
LGRSNRNQHS LKVKQPLAEL VLLEHNENDM DWESYRDIVM DELNVKAFHV 850
ELDETKYTSY QLKLNFKKAG PKFGKNVNAV NGWLKQLSQD EVQNFVSTER 900
AVYEAASGEE VVVTTEDVLV EKVAKSGFSN TTNGQYTVML DTNVTEELLQ 950
EGVAREFIRA VQEYRKQLNL PVNLRVDVIL DTEEELQQTL TNHKELLEEN 1000
LLVKQFTFGH LTNEDDELSL GETKVRIKLS AAQ 1033
Length:1,033
Mass (Da):118,313
Last modified:July 19, 2004 - v1
Checksum:i9172970FA3D595E4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017355 Genomic DNA. Translation: AAT59742.1.
RefSeqiYP_036324.1. NC_005957.1.

Genome annotation databases

EnsemblBacteriaiAAT59742; AAT59742; BT9727_1995.
GeneIDi2858823.
KEGGibtk:BT9727_1995.
PATRICi18984741. VBIBacThu119411_2099.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017355 Genomic DNA. Translation: AAT59742.1 .
RefSeqi YP_036324.1. NC_005957.1.

3D structure databases

ProteinModelPortali Q6HJF2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 281309.BT9727_1995.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAT59742 ; AAT59742 ; BT9727_1995 .
GeneIDi 2858823.
KEGGi btk:BT9727_1995.
PATRICi 18984741. VBIBacThu119411_2099.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi SEGMEPN.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BTHU281309:GJID-2078-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 97-27.

Entry informationi

Entry nameiSYI2_BACHK
AccessioniPrimary (citable) accession number: Q6HJF2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 19, 2004
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi