Reviewed, UniProtKB/Swiss-Prot Q6HJC7 (TYSY_BACHK)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BT9727_2020

Organism

Bacillus thuringiensis subsp. konkukian [Complete proteome] [HAMAP]

Taxonomic identifier

180856 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Thymidylate synthase HAMAP MF_00008		PRO_0000140925
Sites
	Active site	200	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6HJC7-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 6E1FD2DCD1811F18	FASTA	318	36,893
	10 20 30 40 50 60 MKHAENEYLN LCRHVMEHGT KKEDRTGTGT VSVFGYQMRF DLSKGFPLLT TKRVPFRLVA 70 80 90 100 110 120 SELLWFMKGD TNIRYLLQHN NNIWNEWAFK SWVESDEYTG PDMIDFGLRS QQDEEFKVQY 130 140 150 160 170 180 DEQMELFKKN VLEDDEFSNK YGYLGDVYGK QWRAWKTTAG ETLDQLKDVI EMIKKTPDSR 190 200 210 220 230 240 RLIVSAWNPE DVPSMALPPC HTLFQFYVAD GKLSCQLYQR SGDIFLGIPF NIASYSLLTH 250 260 270 280 290 300 LIAHECGLEV GEFVHTIGDA HIYTNHFEQV EKQLAREPRP FPKLTLNPDV KSVFDFEMED 310 LTIEGYDPHP AIKAPVAV

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 97-27.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017355 Genomic DNA. Translation: AAT59764.1.
RefSeq	YP_036349.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2854796.
GenomeReviews	Gene locus BT9727_2020 in contig AE017355_GR.
KEGG	btk:BT9727_2020.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6HJC7.
OMA	LGPVYGH.
Enzyme and pathway databases
BioCyc	BTHU281309:BT9727_2020-MON.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACHK

Accession

Primary (citable) accession number: Q6HJC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)