Q6HHF8 (AROA_BACHK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BT9727_2694

Organism

Bacillus thuringiensis subsp. konkukian (strain 97-27) [Complete proteome] [HAMAP]

Taxonomic identifier

281309 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus thuringiensis › Bacillus thuringiensis subsp. konkukian

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP-Rule MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP-Rule MF_00210
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00210
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP-Rule MF_00210		PRO_0000088224

Sequences

	Sequence		Length	Mass (Da)
	Q6HHF8 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: D8B3CC699D0DE108	FASTA	429	45,156
	10 20 30 40 50 60 MKERTIQPVN NGLNGNITIP GDKSISHRAV MFGAIAEGTT TIKGFLPGAD CLSTISCFKE 70 80 90 100 110 120 MGVDIVQDGD EVTVVGKGLE GLQEPKAVLD VGNSGTTIRL MSGILANTPF FSCVQGDASI 130 140 150 160 170 180 AKRPMKRVTN PLKQMGANID GREEGTFTPL TVRGGDLKAI EYTSPVASAQ VKSAILLAGL 190 200 210 220 230 240 RAEGVTAVTE PHISRDHTER MLEAFGVKVT REGKTVKLAG GQKLTATDVQ VPGDVSSAAF 250 260 270 280 290 300 FLVAGAIIPN SKLVLENVGM NPTRTGIIDV LEKMGATFTV EPINEGASEP AANITIETSS 310 320 330 340 350 360 LKGIEIGGDI IPRLIDEIPV IALAATQAEG ITVIKDAHEL KVKETNRIDT VVAELTKLGA 370 380 390 400 410 420 RIEATDDGMI IYGKSALKGN TVNSYGDHRI GMMLAIAGCI AEGKTIIEDA EAVGVSYPTF FEELQKLAK

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 97-27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017355 Genomic DNA. Translation: AAT61256.1.
RefSeq	YP_037018.1. NC_005957.1.
3D structure databases
ProteinModelPortal	Q6HHF8.
SMR	Q6HHF8. Positions 6-428.
ModBase	Search...
Protein-protein interaction databases
STRING	281309.BT9727_2694.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAT61256; AAT61256; BT9727_2694.
GeneID	2853981.
KEGG	btk:BT9727_2694.
PATRIC	18986267. VBIBacThu119411_2860.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
HOGENOM	HOG000247371.
KO	K00800.
OMA	HYHSPIA.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	BTHU281309:GJID-2772-MONOMER.
UniPathway	UPA00053; UER00089.
Family and domain databases
Gene3D	3.65.10.10. 2 hits.
HAMAP	MF_00210. EPSP_synth.
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BACHK

Accession

Primary (citable) accession number: Q6HHF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	July 19, 2004
Last modified:	May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program