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Protein

3-phosphoshikimate 1-carboxyvinyltransferase

Gene

aroA

Organism
Bacillus thuringiensis subsp. konkukian (strain 97-27)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.UniRule annotation

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei28 – 281Shikimate-3-phosphateUniRule annotation
Binding sitei123 – 1231PhosphoenolpyruvateUniRule annotation
Active sitei316 – 3161Proton acceptorUniRule annotation
Binding sitei343 – 3431Shikimate-3-phosphateUniRule annotation
Active sitei344 – 3441Proton donorUniRule annotation
Binding sitei347 – 3471PhosphoenolpyruvateUniRule annotation
Binding sitei389 – 3891PhosphoenolpyruvateUniRule annotation

GO - Molecular functioni

  1. 3-phosphoshikimate 1-carboxyvinyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. aromatic amino acid family biosynthetic process Source: UniProtKB-HAMAP
  2. chorismate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciBTHU281309:GJID-2772-MONOMER.
UniPathwayiUPA00053; UER00089.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation (EC:2.5.1.19UniRule annotation)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthaseUniRule annotation
Short name:
EPSP synthaseUniRule annotation
Short name:
EPSPSUniRule annotation
Gene namesi
Name:aroAUniRule annotation
Ordered Locus Names:BT9727_2694
OrganismiBacillus thuringiensis subsp. konkukian (strain 97-27)
Taxonomic identifieri281309 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000001301 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4294293-phosphoshikimate 1-carboxyvinyltransferasePRO_0000088224Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi281309.BT9727_2694.

Structurei

3D structure databases

ProteinModelPortaliQ6HHF8.
SMRiQ6HHF8. Positions 6-428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 242Shikimate-3-phosphate bindingUniRule annotation
Regioni93 – 964PhosphoenolpyruvateUniRule annotation

Sequence similaritiesi

Belongs to the EPSP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0128.
HOGENOMiHOG000247371.
KOiK00800.
OMAiGADIEWG.
OrthoDBiEOG6Z6FZ4.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6HHF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKERTIQPVN NGLNGNITIP GDKSISHRAV MFGAIAEGTT TIKGFLPGAD
60 70 80 90 100
CLSTISCFKE MGVDIVQDGD EVTVVGKGLE GLQEPKAVLD VGNSGTTIRL
110 120 130 140 150
MSGILANTPF FSCVQGDASI AKRPMKRVTN PLKQMGANID GREEGTFTPL
160 170 180 190 200
TVRGGDLKAI EYTSPVASAQ VKSAILLAGL RAEGVTAVTE PHISRDHTER
210 220 230 240 250
MLEAFGVKVT REGKTVKLAG GQKLTATDVQ VPGDVSSAAF FLVAGAIIPN
260 270 280 290 300
SKLVLENVGM NPTRTGIIDV LEKMGATFTV EPINEGASEP AANITIETSS
310 320 330 340 350
LKGIEIGGDI IPRLIDEIPV IALAATQAEG ITVIKDAHEL KVKETNRIDT
360 370 380 390 400
VVAELTKLGA RIEATDDGMI IYGKSALKGN TVNSYGDHRI GMMLAIAGCI
410 420
AEGKTIIEDA EAVGVSYPTF FEELQKLAK
Length:429
Mass (Da):45,156
Last modified:July 19, 2004 - v1
Checksum:iD8B3CC699D0DE108
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017355 Genomic DNA. Translation: AAT61256.1.
RefSeqiWP_000664618.1. NC_005957.1.
YP_037018.1. NC_005957.1.

Genome annotation databases

EnsemblBacteriaiAAT61256; AAT61256; BT9727_2694.
GeneIDi2853981.
KEGGibtk:BT9727_2694.
PATRICi18986267. VBIBacThu119411_2860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017355 Genomic DNA. Translation: AAT61256.1.
RefSeqiWP_000664618.1. NC_005957.1.
YP_037018.1. NC_005957.1.

3D structure databases

ProteinModelPortaliQ6HHF8.
SMRiQ6HHF8. Positions 6-428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi281309.BT9727_2694.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT61256; AAT61256; BT9727_2694.
GeneIDi2853981.
KEGGibtk:BT9727_2694.
PATRICi18986267. VBIBacThu119411_2860.

Phylogenomic databases

eggNOGiCOG0128.
HOGENOMiHOG000247371.
KOiK00800.
OMAiGADIEWG.
OrthoDBiEOG6Z6FZ4.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00089.
BioCyciBTHU281309:GJID-2772-MONOMER.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 97-27.

Entry informationi

Entry nameiAROA_BACHK
AccessioniPrimary (citable) accession number: Q6HHF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 19, 2004
Last modified: April 29, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.