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Q6HEZ6

- SYP1_BACHK

UniProt

Q6HEZ6 - SYP1_BACHK

Protein

Proline--tRNA ligase 1

Gene

proS1

Organism
Bacillus thuringiensis subsp. konkukian (strain 97-27)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

    Catalytic activityi

    ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. proline-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. prolyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBTHU281309:GJID-3634-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligase 1UniRule annotation (EC:6.1.1.15UniRule annotation)
    Alternative name(s):
    Prolyl-tRNA synthetase 1UniRule annotation
    Short name:
    ProRS 1UniRule annotation
    Gene namesi
    Name:proS1UniRule annotation
    Ordered Locus Names:BT9727_3560
    OrganismiBacillus thuringiensis subsp. konkukian (strain 97-27)
    Taxonomic identifieri281309 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
    ProteomesiUP000001301: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 566566Proline--tRNA ligase 1PRO_0000248649Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi281309.BT9727_3560.

    Structurei

    3D structure databases

    ProteinModelPortaliQ6HEZ6.
    SMRiQ6HEZ6. Positions 1-565.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0442.
    HOGENOMiHOG000076894.
    KOiK01881.
    OMAiCDTIEKL.
    OrthoDBiEOG6TTVMR.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPiMF_01569. Pro_tRNA_synth_type1.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q6HEZ6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQSMVFSPT LREVPADAEI KSHQLLLRAG FMRQNASGIY SFLPFGLKVL    50
    HKVERIVREE MERAGAVELL MPAMQAAELW QESGRWYSYG SELMRMKDRN 100
    AREFALGATH EEVITDLVRD EVKSYKKLPL TLYQIQTKFR DEQRPRFGLL 150
    RGREFLMKDA YSFHATQESL DEVYDRLYKA YSNIFARCGL NFRAVIADSG 200
    AMGGKDTHEF MVLSDVGEDT IAYSDTSDYA ANIEMAPVVA TYTKSDEAEK 250
    ELEKVATPDQ KAIEEVSAFL NIEADKCIKS MVFKVDEKLV VVLVRGDHEV 300
    NDVKVKNVYG ASVVELASHE EIKELLNCEV GSLGPIGVNG DIEIIADHAV 350
    ASIVNGCSGA NEEGFHYVNV NPERDFKVSQ YTDLRFIQEG DQSPDGNGTI 400
    LFARGIEVGH VFKLGTRYSE AMNATFLDEN GKTQPLIMGC YGIGVSRTVA 450
    AIAEQFNDEN GLVWPKAVAP FHVHVIPVNM KSDAQREMGE NIYNSLQEQG 500
    YEVLLDDRAE RAGVKFADAD LFGLPVRVTV GKKADEGIVE VKVRATGESE 550
    EVKVEELQTY IANILK 566
    Length:566
    Mass (Da):63,193
    Last modified:July 19, 2004 - v1
    Checksum:i44C3A84E3BC060CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017355 Genomic DNA. Translation: AAT63899.1.
    RefSeqiYP_037880.1. NC_005957.1.

    Genome annotation databases

    EnsemblBacteriaiAAT63899; AAT63899; BT9727_3560.
    GeneIDi2856218.
    KEGGibtk:BT9727_3560.
    PATRICi18988144. VBIBacThu119411_3798.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017355 Genomic DNA. Translation: AAT63899.1 .
    RefSeqi YP_037880.1. NC_005957.1.

    3D structure databases

    ProteinModelPortali Q6HEZ6.
    SMRi Q6HEZ6. Positions 1-565.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 281309.BT9727_3560.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAT63899 ; AAT63899 ; BT9727_3560 .
    GeneIDi 2856218.
    KEGGi btk:BT9727_3560.
    PATRICi 18988144. VBIBacThu119411_3798.

    Phylogenomic databases

    eggNOGi COG0442.
    HOGENOMi HOG000076894.
    KOi K01881.
    OMAi CDTIEKL.
    OrthoDBi EOG6TTVMR.

    Enzyme and pathway databases

    BioCyci BTHU281309:GJID-3634-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPi MF_01569. Pro_tRNA_synth_type1.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view ]
    PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view ]
    PRINTSi PR01046. TRNASYNTHPRO.
    SUPFAMi SSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 97-27.

    Entry informationi

    Entry nameiSYP1_BACHK
    AccessioniPrimary (citable) accession number: Q6HEZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3