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Q6HEY2 (Q6HEY2_BACHK) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesSubmitted name:
DNA topoisomerase I EMBL AAT60605.1
EC=5.99.1.2 EMBL AAT60605.1
Gene names
Name:topA EMBL AAT60605.1
Ordered Locus Names:BT9727_3574
OrganismBacillus thuringiensis subsp. konkukian (strain 97-27) [Complete proteome] [HAMAP]
Taxonomic identifier281309 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length692 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. SAAS SAAS005733

Sequence similarities

Contains 1 Toprim domain. SAAS SAAS023406

Sequences

Sequence LengthMass (Da)Tools
Q6HEY2 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 152A4BC459804EEF

FASTA69279,078
        10         20         30         40         50         60 
MSDYLVIVES PSKAKTIEKY LGKKYKVVAS MGHVRDLPKS QMGIEVKNNF TPKYITIRGK 

        70         80         90        100        110        120 
GPVLKDLKSA AKKAKKVYLA ADPDREGEAI AWHLANTLNV DVESDCRVVF NEITKDAIKE 

       130        140        150        160        170        180 
SFKHPRAINM DLVDAQQARR ILDRLVGYNI SPLLWKKVKK GLSAGRVQSV AVRLIIERER 

       190        200        210        220        230        240 
EIQSFEPEEF WTIKTEFVKG KDTFEASFYG VDGEKVQLTN ETQVNEIIEQ LKDNAFSVEN 

       250        260        270        280        290        300 
VTRKERKRNP ALPFTTSSLQ QEAARKLNMR AKKTMMLAQQ LYEGIDLGKQ GTVGLITYMR 

       310        320        330        340        350        360 
TDSTRISETA QTEARTYITE AYGTEYIGAE KKKETKKSNA QDAHEAIRPT SVMRKPEELK 

       370        380        390        400        410        420 
SFLSRDQLRL YKLIWERFVA SQMASAIMDT VTARLINNNV QFRASGSVVK FPGFMKVYVE 

       430        440        450        460        470        480 
SKDDGAEEKD KMLPPLEVGE TVFSKDLEPK QHFTQPPPRY TEARLVRTLE ELGIGRPSTY 

       490        500        510        520        530        540 
VPTLETIQKR GYVGLDNKRF VPTELGEIVI ELILEFFPEI INIEFTANME QSLDEIEEGN 

       550        560        570        580        590        600 
ANWVKIVDDF YVGFEPRLEK AEKEMREVEI KDEPAGEDCE LCNHPMVFKM GKYGKFMACS 

       610        620        630        640        650        660 
NFPDCRNTKP IVKEIGVTCP KCDKGQIIER RSNKKKRLFY GCGTYPECDF VSWDKPIGRK 

       670        680        690 
CPKCEGMLVE KKLKKGVQVQ CISCDYEEEQ QM

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017355 Genomic DNA. Translation: AAT60605.1.
RefSeqYP_037894.1. NC_005957.1.

3D structure databases

ProteinModelPortalQ6HEY2.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000075830; EBBACP00000073916; EBBACG00000075821.
GeneID2858382.
GenomeReviewsGene locus BT9727_3574 in contig AE017355_GR.
KEGGbtk:BT9727_3574.
NMPDRfig|281309.1.peg.3534.
PATRIC18988172. VBIBacThu119411_3812.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00070000032133.
HOGENOMHBG512935.
OMAEEYWSID.
ProtClustDBPRK05582.

Family and domain databases

InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR006171. Toprim_domain.
[Graphical view]
Gene3DG3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits.
G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KOK03168.
PANTHERPTHR11390. Topo_IA. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. Topo_IA_core. 1 hit.
TIGRFAMsTIGR01051. TopA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ6HEY2_BACHK
AccessionPrimary (citable) accession number: Q6HEY2
Entry history
Integrated into UniProtKB/TrEMBL: July 19, 2004
Last sequence update: July 19, 2004
Last modified: January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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