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Protein

Isoleucine--tRNA ligase 1

Gene

ileS1

Organism
Bacillus thuringiensis subsp. konkukian (strain 97-27)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei552Aminoacyl-adenylateUniRule annotation1
Binding sitei596ATPUniRule annotation1
Metal bindingi888ZincUniRule annotation1
Metal bindingi891ZincUniRule annotation1
Metal bindingi908ZincUniRule annotation1
Metal bindingi911ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase 1UniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase 1UniRule annotation
Short name:
IleRS 1UniRule annotation
Gene namesi
Name:ileS1UniRule annotation
Ordered Locus Names:BT9727_3637
OrganismiBacillus thuringiensis subsp. konkukian (strain 97-27)
Taxonomic identifieri281309 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
Proteomesi
  • UP000001301 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000983521 – 921Isoleucine--tRNA ligase 1Add BLAST921

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ6HER9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi593 – 597"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiTHGFIVD.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6HER9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYKNTLLMP KTEFPMRGNL PKREPAMQEK WAEMNIYEKV QEHTKGRPLF
60 70 80 90 100
VLHDGPPYAN GDIHMGHALN KVLKDFIVRY KSMTGFCAPY VPGWDTHGLP
110 120 130 140 150
IEQALTNKGV KRKEMTVAEF RKLCAEYAYE QVERQREQFK RLGVRADWDN
160 170 180 190 200
PYITLEPAYE AQQIKVFGDM AKKGYIYKGQ KPVYWSPTSE SALAEAEIEY
210 220 230 240 250
QDKKSASIYV AFPVKDGKNV LEGDEKYIIW TTTPWTLPAN LGISVHPELE
260 270 280 290 300
YAIVKVNDEK YIIASELFET VAKTLEWENA EVVKTVKGSE LEYTVAKHPF
310 320 330 340 350
YDRDSLVMLG DHVTTDAGTG CVHTAPGHGE DDFVVGKKYG LEVLCPVDDK
360 370 380 390 400
GVLTEEAPGF EGLFYDKANK PITEKLEEVG ALLKLTFITH SYPHDWRTKK
410 420 430 440 450
PIIFRATAQW FASIEAFRKE LLEAVAETKW VPAWGETRLH NMVRDRGDWC
460 470 480 490 500
ISRQRAWGVP IPVFYAENGD PIITDETINH VADLFREHGS NVWFEREAKD
510 520 530 540 550
LLPEGFTHPG SPNGEFRKET DIMDVWFDSG SSHQAVLEER DDLQRPADLY
560 570 580 590 600
LEGSDQYRGW FNSSLSTAVA VTGKAPYKGV LSHGFVLDGE GRKMSKSIGN
610 620 630 640 650
IVVPKKIMDQ LGGDILRLWV SSVDYQSDVR ISDDILKQVA EVYRKIRNTF
660 670 680 690 700
RFLLGNLDDF KPSENTVAVA ELREVDRYML VKLNDLITKV KEAYETYDFA
710 720 730 740 750
AVYHAIHNFC TIDLSSFYLD FAKDILYIEG ANHEDRRAIQ TVLYDVLVAL
760 770 780 790 800
TKLVTPILPH TADEVWPYIP GVTEESVQLT DMPEAVQLDD AEALKTKWDA
810 820 830 840 850
FMTLRDDVLK ALEVARNEKV IGKSLNASIT LYPTAEMKAM LESINEDLKQ
860 870 880 890 900
LFIVSEYKLG GMMEEAPADA PKYEHTAVVV AQATGETCER CWVVSETIGK
910 920
DAEHETLCER CATVVKENYV K
Length:921
Mass (Da):104,634
Last modified:July 19, 2004 - v1
Checksum:iABF082F44E94A891
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017355 Genomic DNA. Translation: AAT63892.1.
RefSeqiWP_000455930.1. NC_005957.1.
YP_037957.1. NC_005957.1.

Genome annotation databases

EnsemblBacteriaiAAT63892; AAT63892; BT9727_3637.
GeneIDi2857105.
KEGGibtk:BT9727_3637.
PATRICi18988298. VBIBacThu119411_3875.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017355 Genomic DNA. Translation: AAT63892.1.
RefSeqiWP_000455930.1. NC_005957.1.
YP_037957.1. NC_005957.1.

3D structure databases

ProteinModelPortaliQ6HER9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT63892; AAT63892; BT9727_3637.
GeneIDi2857105.
KEGGibtk:BT9727_3637.
PATRICi18988298. VBIBacThu119411_3875.

Phylogenomic databases

HOGENOMiHOG000246402.
KOiK01870.
OMAiTHGFIVD.

Family and domain databases

CDDicd07960. Anticodon_Ia_Ile_BEm. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR033708. Anticodon_Ile_BEm.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_FPG/IleRS.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYI1_BACHK
AccessioniPrimary (citable) accession number: Q6HER9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.