Reviewed, UniProtKB/Swiss-Prot Q6HE47 (BIOD_BACHK)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	BT9727_3860

Organism

Bacillus thuringiensis subsp. konkukian [Complete proteome] [HAMAP]

Taxonomic identifier

180856 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000302480
Regions
	Nucleotide binding	8 – 16	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6HE47-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 9EA37BD78E14EFBF	FASTA	242	26,259
	10 20 30 40 50 60 MSGFFITATD TEVGKTVVAG ALAGVFRELG YNIGVYKALQ SGHVASNPEG DAARLKVLSG 70 80 90 100 110 120 VPTKEDEICP YSIEEPLAPR LAMKRAGRAV TLKDIIHHYN ERLKEFNSLF VEGAGGLAVP 130 140 150 160 170 180 YTEDALVIDF AKELQLPLIV VARPTLGTVN HTVLTIAYAK ANGLTVAGVI LSGCKECEME 190 200 210 220 230 240 RVQENKVMIE ELSGVPVLGL LPFFEGEFTK EEVLESAKEY IMISKLEEFI RNESTVAHTS SN

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 97-27.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017355 Genomic DNA. Translation: AAT60741.1.
RefSeq	YP_038179.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2856934.
GenomeReviews	Gene locus BT9727_3860 in contig AE017355_GR.
KEGG	btk:BT9727_3860.
NMPDR	fig|281309.1.peg.3819.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6HE47.
OMA	CLIEGAG.
Enzyme and pathway databases
BioCyc	BTHU281309:BT9727_3860-MON.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BACHK

Accession

Primary (citable) accession number: Q6HE47

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 19, 2004
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)