Q6HE30 (ARGB_BACHK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	BT9727_3877

Organism

Bacillus thuringiensis subsp. konkukian (strain 97-27) [Complete proteome] [HAMAP]

Taxonomic identifier

281309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.
Sequence caution	The sequence AAT60753.1 differs from that shown. Reason: Erroneous termination at position 15. Translated as Asp.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000112583
Regions
	Region	40 – 41	2	Substrate binding By similarity
Sites
	Binding site	62	1	Substrate By similarity
	Binding site	153	1	Substrate By similarity
	Site	8	1	Transition state stabilizer By similarity
	Site	212	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6HE30 [UniParc]. Last modified February 1, 2005. Version 2. Checksum: 9CBD601A89210D3D	FASTA	255	27,397
	10 20 30 40 50 60 MNDYIVVKCG GSMLDQLNDV FFDCIKKLQQ QYKVVIVHGG GPEIDAKLKD CNINVEKRDG 70 80 90 100 110 120 LRVTPKEVMD VVQMVLCGST NKKFVMNLQK HNLLAVGCSG CDGKLLQVQP VSEEIGYVGE 130 140 150 160 170 180 VSYVETALLK GLINMNYIPV IAPIGIHDNE IYNINADTAA AGIAAALSAK ELILITDVDG 190 200 210 220 230 240 ILHEGKLVKK TDESEIATFI EKGVITGGMI PKVQAALASL KMGVQKISIV NGTKDFTEDT 250 GECIGTTVTR GVSIV

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 97-27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017355 Genomic DNA. Translation: AAT60753.1. Sequence problems.
RefSeq	YP_038196.1. NC_005957.1.
3D structure databases
ProteinModelPortal	Q6HE30.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000073936; EBBACP00000072022; EBBACG00000073927.
GeneID	2854946.
GenomeReviews	Gene locus BT9727_3877 in contig AE017355_GR.
KEGG	btk:BT9727_3877.
NMPDR	fig|281309.1.peg.3836.
PATRIC	18988818. VBIBacThu119411_4135.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000001971.
HOGENOM	HBG497643.
OMA	CNINVEK.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	BTHU281309:BT9727_3877-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_BACHK

Accession

Primary (citable) accession number: Q6HE30

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program