Reviewed, UniProtKB/Swiss-Prot Q6HDI8 (AROE_BACHK)

Names and origin

Protein names

Recommended name:
    Shikimate dehydrogenase
    EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	BT9727_4070

Organism

Bacillus thuringiensis subsp. konkukian [Complete proteome] [HAMAP]

Taxonomic identifier

180856 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Shikimate + NADP(+) = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and PEP: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000021262
Regions
	Nucleotide binding	127 – 131	5	NADP By similarity
Sites
	Active site	66	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6HDI8-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: D3D28723C9F1A548	FASTA	277	30,123
	10 20 30 40 50 60 MKQLYGVIGN PIGHSLSPVM HNDAFEHLNM DAHYHAFLVK EEVLGEAVRG LKALGISGFN 70 80 90 100 110 120 VTTPHKVAIM DYLDEIDPLA KQIGAVNTVV HKDGKLIGYN TDGIGFVRAL QSISSEPLQE 130 140 150 160 170 180 KRILLLGAGG ASRAIYFSLA DAGVKEIDVA NRTVDKAKEL IAACTATVHS VALSLEKATK 190 200 210 220 230 240 EQGSYDIIIQ TTTIGMHPRV EHTPLQISSL KKGTIVSDII YNPFETKILC EAKEQGAIIQ 250 260 270 NGIDMFVYQG ALAFEMWTGC VPNIERMKQL VIRKLGG

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 97-27.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017355 Genomic DNA. Translation: AAT63595.1.
RefSeq	YP_038388.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2855841.
GenomeReviews	Gene locus BT9727_4070 in contig AE017355_GR.
KEGG	btk:BT9727_4070.
NMPDR	fig|281309.1.peg.4028.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q6HDI8.
Enzyme and pathway databases
BioCyc	BTHU281309:BT9727_4070-MON.
Family and domain databases
HAMAP	MF_00222. [Tree]
InterPro	IPR016040. NAD(P)-bd. IPR011342. Quinate/shikimate_5-DHase. IPR013708. Shikimate_DHase-bd_N. IPR006151. Shikm_DHase/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_BACHK

Accession

Primary (citable) accession number: Q6HDI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 19, 2004
Last modified:	November 25, 2008
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)