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Q6HDA9 (TGT_BACHK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29
Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase
Gene names
Name:tgt
Ordered Locus Names:BT9727_4150
OrganismBacillus thuringiensis subsp. konkukian (strain 97-27) [Complete proteome] [HAMAP]
Taxonomic identifier281309 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP MF_00168

Catalytic activity

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168

[tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00168

Pathway

tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processqueuosine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

queuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 379379Queuine tRNA-ribosyltransferase HAMAP MF_00168
PRO_0000135449

Sites

Active site941Nucleophile By similarity
Metal binding3061Zinc By similarity
Metal binding3081Zinc By similarity
Metal binding3111Zinc By similarity
Metal binding3371Zinc By similarity
Binding site951Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6HDA9 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 6DE1E29B88568644

FASTA37943,227
        10         20         30         40         50         60 
MTAIRYEFIK TCKQTGARLG RVHTPHGSFD TPTFMPVGTL ATVKTMSPEE LKAMDSGIIL 

        70         80         90        100        110        120 
SNTYHLWLRP GHEIIREAGG LHKFMNWDRA ILTDSGGFQV FSLSDFRRIE EEGVHFRNHL 

       130        140        150        160        170        180 
NGDKLFLSPE KAMEIQNALG SDIMMAFDEC PPFPATFEYM KKSVERTSRW AERCLKAHER 

       190        200        210        220        230        240 
PQDQGLFGIV QGGEFEELRR QSAKDLVSMD FPGYAVGGLS VGEPKDIMNR VLEFTTPLLP 

       250        260        270        280        290        300 
DNKPRYLMGV GSPDSLIDGA IRGIDMFDCV LPTRIARNGT CMTSEGRLVV KNAKFARDFG 

       310        320        330        340        350        360 
PLDPNCDCYT CKNYSRAYIR HLMKCDETFG IRLTSYHNLH FLLNLMEQVR QAIREDRLGD 

       370 
FREEFFEQYG FNKPNAKNF

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE017355 Genomic DNA. Translation: AAT63704.1.
RefSeqYP_038467.1. NC_005957.1.

3D structure databases

ProteinModelPortalQ6HDA9.
SMRQ6HDA9. Positions 5-369.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000073796; EBBACP00000071882; EBBACG00000073787.
GeneID2855578.
GenomeReviewsGene locus BT9727_4150 in contig AE017355_GR.
KEGGbtk:BT9727_4150.
PATRIC18989407. VBIBacThu119411_4428.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000001431.
HOGENOMHBG629929.
OMAFMPVGTV.
ProtClustDBPRK00112.

Enzyme and pathway databases

BioCycBTHU281309:BT9727_4150-MONOMER.

Family and domain databases

HAMAPMF_00168. Q_tRNA_Tgt.
[Tree]
InterProIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans.
[Graphical view]
Gene3DG3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
KOK00773.
PANTHERPTHR11962. tRNA_ribo_trans. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMSSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. Tgt_general. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTGT_BACHK
AccessionPrimary (citable) accession number: Q6HDA9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 19, 2004
Last modified: January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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