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Protein

NAD kinase 2

Gene

nadK2

Organism
Bacillus thuringiensis subsp. konkukian (strain 97-27)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei52 – 521Proton acceptorUniRule annotation
Binding sitei151 – 1511NADUniRule annotation
Binding sitei153 – 1531NADUniRule annotation
Binding sitei188 – 1881NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi52 – 532NADUniRule annotation
Nucleotide bindingi124 – 1252NADUniRule annotation
Nucleotide bindingi164 – 1696NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBTHU281309:GJID-4447-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinase 2UniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinase 2UniRule annotation
Gene namesi
Name:nadK2UniRule annotation
Ordered Locus Names:BT9727_4376
OrganismiBacillus thuringiensis subsp. konkukian (strain 97-27)
Taxonomic identifieri281309 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group
ProteomesiUP000001301: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267NAD kinase 2PRO_0000229607Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi281309.BT9727_4376.

Structurei

3D structure databases

ProteinModelPortaliQ6HCN5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiSIRHSHE.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6HCN5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADRRNLFFF YGDDKAKLVE KMKPIYRILE ENGFTILDHP KNANAIVSVG
60 70 80 90 100
DDATFLQAVR KTGFREDCLY AGISTKDEIS FYCDFHIDHV DTALQEITKN
110 120 130 140 150
EIEVRKYPTI EVDVDGSTSF HCLNEFSLRS SIIKTFVVDV HVDDLYFETF
160 170 180 190 200
RGDGLVVSTP TGSTAYNKSL RGAVVDPLIP CFQVSELASL NNNTYRTLGS
210 220 230 240 250
PFILNHERTL TLKLRPDGND YPVIGMDNEA LSIKQVEKAV VRLSDKQIKT
260
VKLKNNSFWE KVQRTFL
Length:267
Mass (Da):30,365
Last modified:July 19, 2004 - v1
Checksum:i22C017138F19C795
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017355 Genomic DNA. Translation: AAT63589.1.
RefSeqiYP_038691.1. NC_005957.1.

Genome annotation databases

EnsemblBacteriaiAAT63589; AAT63589; BT9727_4376.
GeneIDi2856194.
KEGGibtk:BT9727_4376.
PATRICi18989881. VBIBacThu119411_4664.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017355 Genomic DNA. Translation: AAT63589.1.
RefSeqiYP_038691.1. NC_005957.1.

3D structure databases

ProteinModelPortaliQ6HCN5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi281309.BT9727_4376.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT63589; AAT63589; BT9727_4376.
GeneIDi2856194.
KEGGibtk:BT9727_4376.
PATRICi18989881. VBIBacThu119411_4664.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiSIRHSHE.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciBTHU281309:GJID-4447-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 97-27.

Entry informationi

Entry nameiNADK2_BACHK
AccessioniPrimary (citable) accession number: Q6HCN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 19, 2004
Last modified: January 7, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.