Q6HCJ2 (MURC_BACHK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BT9727_4420

Organism

Bacillus thuringiensis subsp. konkukian (strain 97-27) [Complete proteome] [HAMAP]

Taxonomic identifier

281309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182052
Regions
	Nucleotide binding	108 – 114	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q6HCJ2 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: B3FFABA398462CB9	FASTA	436	49,229
	10 20 30 40 50 60 MTVYHFVGIK GTGMSSLAQI LHDMKHTVQG SDYEKRFFTQ TALEKRSISI LPFDKSNVKE 70 80 90 100 110 120 GQVIIAGNAF PDTHEEIVAA KELNIPVHRY HHFLGDLMNQ YTSVAVTGAH GKTSTTGLLA 130 140 150 160 170 180 HVMQGAHPTS YLIGDGTGHG VENSKYFVFE ACEYRRHFLS YNPDYAIMTN IDFDHPDYFT 190 200 210 220 230 240 DINDVFSAFQ EMALQVKKGI IACGDDEELQ KIQAKVPVIF YGFGEDNDFQ ARNIQKRTDG 250 260 270 280 290 300 TIFDVFVRNT YYDTFKITGY GNHSVLNALA VIALCHYENV DVEAVKHQLT TFEGVKRRFN 310 320 330 340 350 360 EKPMGEQVII DDYAHHPTEI NATIEAARQK HPEREIVAVF QPHTFSRTEK FLDEFAESLS 370 380 390 400 410 420 KADQVYLCDI FGSARENKGE LTIEDLQKRI DGAELITDTT TDVLKKHKNG VLIFMGAGDI 430 QKFEAAYVKE VQVAEK

References

[1]

"Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis." Han C.S., Xie G., Challacombe J.F., Altherr M.R., Bhotika S.S., Bruce D., Campbell C.S., Campbell M.L., Chen J., Chertkov O., Cleland C., Dimitrijevic M., Doggett N.A., Fawcett J.J., Glavina T., Goodwin L.A., Hill K.K., Hitchcock P. , Jackson P.J., Keim P., Kewalramani A.R., Longmire J., Lucas S., Malfatti S., McMurry K., Meincke L.J., Misra M., Moseman B.L., Mundt M., Munk A.C., Okinaka R.T., Parson-Quintana B., Reilly L.P., Richardson P., Robinson D.L., Rubin E., Saunders E., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Ticknor L.O., Wills P.L., Brettin T.S., Gilna P. J. Bacteriol. 188:3382-3390(2006) [PubMed: 16621833] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 97-27.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017355 Genomic DNA. Translation: AAT63653.1.
RefSeq	YP_038734.1. NC_005957.1.
3D structure databases
ProteinModelPortal	Q6HCJ2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000075041; EBBACP00000073127; EBBACG00000075032.
GeneID	2853770.
GenomeReviews	Gene locus BT9727_4420 in contig AE017355_GR.
KEGG	btk:BT9727_4420.
PATRIC	18989969. VBIBacThu119411_4708.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000000630.
HOGENOM	HBG476594.
OMA	YQPHRYS.
ProtClustDB	PRK00421.
Enzyme and pathway databases
BioCyc	BTHU281309:BT9727_4420-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BACHK

Accession

Primary (citable) accession number: Q6HCJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 19, 2004
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program