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Q6HA23 (GSHR_KLULA) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione reductase

Short name=GR
Short name=GRase
EC=1.8.1.7
Gene names
Name:GLR1
Ordered Locus Names:KLLA0E24112g
OrganismKluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica) [Complete proteome]
Taxonomic identifier284590 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeKluyveromyces

Protein attributes

Sequence length484 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Maintains high levels of reduced glutathione in the cytosol By similarity.

Catalytic activity

2 glutathione + NADP+ = glutathione disulfide + NADPH.

Cofactor

Binds 1 FAD per subunit By similarity.

Subcellular location

Cytoplasm By similarity.

Miscellaneous

The active site is a redox-active disulfide bond By similarity.

Sequence similarities

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   DomainRedox-active center
   LigandFAD
Flavoprotein
NADP
   Molecular functionOxidoreductase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcell redox homeostasis

Inferred from electronic annotation. Source: InterPro

glutathione metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADP binding

Inferred from electronic annotation. Source: InterPro

flavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

glutathione-disulfide reductase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 484484Glutathione reductase
PRO_0000067968

Regions

Nucleotide binding52 – 609FAD By similarity

Sites

Active site4731Proton acceptor By similarity

Amino acid modifications

Disulfide bond60 ↔ 65Redox-active By similarity

Sequences

Sequence LengthMass (Da)Tools
Q6HA23 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: CEE24F6377CC0ECB

FASTA48453,378
        10         20         30         40         50         60 
MSIRNTINRI IIRRNMSDSI RHYDYLVIGG GSGGVASSRR AASYGAKTLL IEAKAMGGTC 

        70         80         90        100        110        120 
VNKGCVPKKV MWYASDLATR IGHAHSYNLF EDLPLTKENL TFNWPEFKKK RDAYIHRLNG 

       130        140        150        160        170        180 
IYERNLTKEG VDYVYGWASF TVDGKVQVKK ADNCTETYTA DHILVATGGK PIYPAKIPGY 

       190        200        210        220        230        240 
DYGVSSDEFF ELEDQPKKVV VVGAGYIGVE IAGVFNGLGS DSHLVIRGET VLRKFDDCIQ 

       250        260        270        280        290        300 
ETVTDTYIKE GVNIHKSSNV TKVEKDESTG KLNIQLDTGK NIDNVDSLIW TIGRRSLLGL 

       310        320        330        340        350        360 
GLENIGVKLD AKEQIVVDEY QNSSVKNVYS LGDVVGKVEL TPVAIAAGRK LSNRLFGPEK 

       370        380        390        400        410        420 
FKNQKQDYEN VPSVVFSHPE AGSIGLSERE AIEKFGKDNV KVYNSKFNAM YYAMMEEKDK 

       430        440        450        460        470        480 
TPTRYKLVCT GEEEKVVGLH IIGDSSAEIL QGFGVAIKMG ATKADFDSCV AIHPTSAEEL 


VTLT 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ504414 Genomic DNA. Translation: CAD43213.1.
CR382125 Genomic DNA. Translation: CAH00123.1.
RefSeqXP_455036.1. XM_455036.1.

3D structure databases

ProteinModelPortalQ6HA23.
SMRQ6HA23. Positions 21-483.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2894346.
KEGGkla:KLLA0E24069g.

Phylogenomic databases

eggNOGCOG1249.
HOGENOMHOG000276712.
KOK00383.
OMAHRQPCKM.
OrthoDBEOG79W9F2.

Family and domain databases

Gene3D3.30.390.30. 1 hit.
InterProIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006322. Glutathione_Rdtase_euk/bac.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSPR00368. FADPNR.
SUPFAMSSF55424. SSF55424. 1 hit.
TIGRFAMsTIGR01421. gluta_reduc_1. 1 hit.
PROSITEPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHR_KLULA
AccessionPrimary (citable) accession number: Q6HA23
Secondary accession number(s): Q6CM03
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 19, 2004
Last modified: November 13, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families