Reviewed, UniProtKB/Swiss-Prot Q6H9K0 (PGLR2_PLAAC)

Names and origin

Protein names

Recommended name:
    Exopolygalacturonase
    EC=3.2.1.67
Alternative name(s):
    Pollen allergen Pla a 2
    Allergen=Pla a 2

Gene names

Name:

plaa2

Organism

Platanus acerifolia (London plane tree)

Taxonomic identifier

140101 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Proteales › Platanaceae › Platanus

Protein attributes

Sequence length	377 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase. Ref.1 UniProtKB P24548
Catalytic activity	((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate. Ref.1
Subunit structure	Monomer. Ref.1
Subcellular location	Secreted By similarity. Secreted › cell wall By similarity.
Tissue specificity	Expressed in pollen and stem, but not in leaves. Ref.1
Post-translational modification	Glycosylated. Ref.1
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.
Biophysicochemical properties	pH dependence: Optimum pH is 4.0. Temperature dependence: Optimum temperature is 50 degrees Celsius.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Disease	Allergen
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW pectin catabolic process Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	IgE binding Ref.1 Inferred from direct assay. Source: UniProtKB galacturan 1,4-alpha-galacturonidase activity Ref.1 Inferred from direct assay. Source: UniProtKB polygalacturonase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 5	›5	Ref.1
	Chain	6 – 377	372	Exopolygalacturonase Ref.1		PRO_0000024821
Regions
	Repeat	159 – 184	26	PbH1 1
	Repeat	186 – 207	22	PbH1 2
	Repeat	209 – 229	21	PbH1 3
	Repeat	239 – 260	22	PbH1 4
	Repeat	269 – 290	22	PbH1 5
Sites
	Active site	200	1	Proton donor By similarity
	Active site	223	1	By similarity UniProtKB P26509
Amino acid modifications
	Glycosylation	211	1	N-linked (GlcNAc...) Potential
	Glycosylation	345	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q6H9K0-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 10E0F271114BA3D3	FASTA	377	39,765
	10 20 30 40 50 60 RGVQSSGSVF NVNDYGAKGA GDISQAVMKA WKAACASQGP STVLIPKGNY NMGEVAMQGP 70 80 90 100 110 120 CKGSKIGFQI DGVVKAPADP SKFKSDGWVS FYRIDGLTVS GTGTLDGQGQ TAWAKNNCDK 130 140 150 160 170 180 NPNCKHAAMN LRFDFLKHAM VRDITSLNSK MFHINVLECE DITFQHVTVT APGTSINTDG 190 200 210 220 230 240 IHVGISKGVT ITNTKIATGD DCISIGPGSQ NVTITQVNCG PGHGISIGSL GRYNNEKEVR 250 260 270 280 290 300 GITVKGCTFS GTMNGVRVKT WPNSPPGAAT DLTFQDLTMN NVQNPVILDQ EYCPYGQCSR 310 320 330 340 350 360 QAPSRIKLSN INFNNIRGTS TGKVAVVIAC SHGMPCSNMK IGEINLSYRG AGGPATSTCS 370 NVKPTFSGKQ VPAIKCA

References

[1]

"Identification of a polygalacturonase as a major allergen (Pla a 2) from Platanus acerifolia pollen." Ibarrola I., Arilla M.C., Martinez A., Asturias J.A. J. Allergy Clin. Immunol. 113:1185-1191(2004) [PubMed: 15208603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 6-25; 130-137; 340-347 AND 349-358, GLYCOSYLATION, CATALYTIC ACTIVITY, SUBUNIT, TISSUE SPECIFICITY, ALLERGENICITY. Tissue: Pollen.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ586898 mRNA. Translation: CAE52833.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.67. 228874.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR2_PLAAC

Accession

Primary (citable) accession number: Q6H9K0
Secondary accession number(s): P82967

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)