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Protein

Exopolygalacturonase

Gene

plaa2

Organism
Platanus acerifolia (London plane tree)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function in depolymerizing pectin during pollen development, germination, and tube growth. Acts as an exo-polygalacturonase.By similarity1 Publication

Catalytic activityi

((1->4)-alpha-D-galacturonide)(n) + H2O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate.1 Publication

pH dependencei

Optimum pH is 4.0.

Temperature dependencei

Optimum temperature is 50 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei200 – 2001Proton donorBy similarity
Active sitei223 – 2231By similarity

GO - Molecular functioni

  • galacturan 1,4-alpha-galacturonidase activity Source: UniProtKB
  • IgE binding Source: UniProtKB
  • polygalacturonase activity Source: InterPro

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • pectin catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.

Names & Taxonomyi

Protein namesi
Recommended name:
Exopolygalacturonase (EC:3.2.1.67)
Alternative name(s):
Pollen allergen Pla a 2
Allergen: Pla a 2
Gene namesi
Name:plaa2Imported
OrganismiPlatanus acerifolia (London plane tree)
Taxonomic identifieri140101 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsProtealesPlatanaceaePlatanus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3426. Pla a 2.0101.
573. Pla a 2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 5›51 Publication
Chaini6 – 377372Exopolygalacturonase1 PublicationPRO_0000024821Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi211 – 2111N-linked (GlcNAc...)Sequence analysis
Glycosylationi345 – 3451N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in pollen and stem, but not in leaves.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

GO - Molecular functioni

  • IgE binding Source: UniProtKB

Structurei

3D structure databases

ProteinModelPortaliQ6H9K0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati159 – 18426PbH1 1Sequence analysisAdd
BLAST
Repeati186 – 20722PbH1 2Sequence analysisAdd
BLAST
Repeati209 – 22921PbH1 3Sequence analysisAdd
BLAST
Repeati239 – 26022PbH1 4Sequence analysisAdd
BLAST
Repeati269 – 29022PbH1 5Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated
Contains 5 PbH1 repeats.Sequence analysis

Keywords - Domaini

Repeat, Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6H9K0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
RGVQSSGSVF NVNDYGAKGA GDISQAVMKA WKAACASQGP STVLIPKGNY
60 70 80 90 100
NMGEVAMQGP CKGSKIGFQI DGVVKAPADP SKFKSDGWVS FYRIDGLTVS
110 120 130 140 150
GTGTLDGQGQ TAWAKNNCDK NPNCKHAAMN LRFDFLKHAM VRDITSLNSK
160 170 180 190 200
MFHINVLECE DITFQHVTVT APGTSINTDG IHVGISKGVT ITNTKIATGD
210 220 230 240 250
DCISIGPGSQ NVTITQVNCG PGHGISIGSL GRYNNEKEVR GITVKGCTFS
260 270 280 290 300
GTMNGVRVKT WPNSPPGAAT DLTFQDLTMN NVQNPVILDQ EYCPYGQCSR
310 320 330 340 350
QAPSRIKLSN INFNNIRGTS TGKVAVVIAC SHGMPCSNMK IGEINLSYRG
360 370
AGGPATSTCS NVKPTFSGKQ VPAIKCA
Length:377
Mass (Da):39,765
Last modified:July 19, 2004 - v1
Checksum:i10E0F271114BA3D3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11Imported

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ586898 mRNA. Translation: CAE52833.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ586898 mRNA. Translation: CAE52833.1.

3D structure databases

ProteinModelPortaliQ6H9K0.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3426. Pla a 2.0101.
573. Pla a 2.
CAZyiGH28. Glycoside Hydrolase Family 28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTiSM00710. PbH1. 5 hits.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR2_PLAAC
AccessioniPrimary (citable) accession number: Q6H9K0
Secondary accession number(s): P82967
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: July 19, 2004
Last modified: January 7, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.