Reviewed, UniProtKB/Swiss-Prot Q6H9I1 (ATG1_BOTFU)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase ATG1
    EC=2.7.11.1
Alternative name(s):
    Autophagy-related protein 1
    Botryotinia fuckeliana putative kinase exons 1-3

Gene names

Name:	atg1
Synonyms:	bpk3

Organism

Botryotinia fuckeliana (Noble rot fungus) (Botrytis cinerea)

Taxonomic identifier

40559 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Leotiomycetes › Helotiales › Sclerotiniaceae › Botryotinia

Protein attributes

Sequence length	952 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serine/threonine protein kinase probably involved in the cytoplasm to vacuole transport (Cvt) and in autophagy, where it may be required for the formation of autophagosomes By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Autophagy Protein transport Transport
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 952	952	Serine/threonine-protein kinase ATG1		PRO_0000085640
Regions
	Domain	23 – 329	307	Protein kinase
	Nucleotide binding	29 – 37	9	ATP By similarity
Sites
	Active site	166	1	Proton acceptor By similarity
	Binding site	52	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6H9I1-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 90AEAE54056440EB	FASTA	952	105,041
	10 20 30 40 50 60 MASTTTSTSS LSSRRQKTGV GSFTINEQIG KGSFATVYRG THMPSGNLVA IKSVNLSRLN 70 80 90 100 110 120 KKLKDNLYVE IEILKSLYHP HIVALIDCRE SASHIHLMME YCELGDLSYF IKKRDRLADN 130 140 150 160 170 180 PTLYDMVQKY PMPVEGGLNQ VVVRHFFKQL SSAMEFLRER DFVHRDVKPQ NLLLIPSPEW 190 200 210 220 230 240 IAKRAKGGPE AMKASKESVV AMVGINSLPM LKLADFGFAR SLPSTSLAET LCGSPLYMAP 250 260 270 280 290 300 EILRYEKYDA RADLWSIGTV LYEMMTGRPP FKAINHVQLL QKIEKNQDEI RFPSRGIYSR 310 320 330 340 350 360 DLKDIVRRLL KKKPEDRITF PEYFAHPVVT EPIPGLVGDD RPKEKSPETS IVRQPSLRDR 370 380 390 400 410 420 QRESPTVKHI DTAYESLITR DIGEQSPRTP NIESNQPFGT PGRSSGRPDS RDRPSPVSAA 430 440 450 460 470 480 TAPNVDTLPR QRDRKDRTEP NYAPIVRTGS TKQRYDEQAN LQPKNEVQSS NSITEAEQDV 490 500 510 520 530 540 RDAREYVLVE KKAVEVNAFA DEMAANPRLG RANSAPKQLP RRHTSMGEPN STTGAVAVPP 550 560 570 580 590 600 SRIVQRASGR AQPDTSSARN SYGSYGKTGS SPSTASAIAK ALQGASVRVF GVSWSPTLIG 610 620 630 640 650 660 KGPSPPQLYN PYPAYPTPNA GLIGDGRPID EDQRVVNIIE DSATRSDVVY GFAEVKYRQL 670 680 690 700 710 720 IPLAPSMNHG LGGPNPERTG DAMDEDDGLT VEAIVNLSEE ALVLYVKSLS LLSKSMDIAG 730 740 750 760 770 780 AWWSRKQRGG IVSGGHTPGS DSSSAAQAGN RINGAVQWVR TRFNEVLEKA ELVRLKLVEA 790 800 810 820 830 840 QKRLPEDHPG HPNNRSTASR LVGGSSTTDG VVLSSGITAE KLMYDRALEM SRTAAINELA 850 860 870 880 890 900 NEDLPGCEIS YTTAIRMLEA VLENDEELIP RKRSSSLRED KEKSEGGEVN GINFGDRKDV 910 920 930 940 950 LKVLQMIRTR LQVLKKKMTA IAKHQSMPPP SSSPRRSYSG GTTPTINNTP PK

References

[1]	"bpk3, a clk-like serine-threonine protein kinase from Botryotinia fuckeliana." Schulze Gronover C., Tudzynski P., Tudzynski B. Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SAS56.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ608273 Genomic DNA. Translation: CAE55218.1.
3D structure databases
SMR	Q6H9I1. Positions 17-332.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.1. 290409.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR020656. Ser/Thr_prot_kinase_ATG1. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit. PTHR22982:SF39. Ser/Thr_prot_kinase_ATG1. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG1_BOTFU

Accession

Primary (citable) accession number: Q6H9I1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	July 19, 2004
Last modified:	January 19, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)