Q6H8D5 (COB22_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coatomer subunit beta'-2

Alternative name(s):
Beta'-coat protein 2
Short name=Beta'-COP 2

Gene names

Ordered Locus Names:	Os02g0209100, LOC_Os02g11840
ORF Names:	OJ1006_A02.24

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	910 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins By similarity.
Subunit structure	Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits By similarity.
Subcellular location	Cytoplasm By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasmic vesicle › COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it By similarity.
Sequence similarities	Belongs to the WD repeat COPB2 family. Contains 9 WD repeats.
Sequence caution	The sequence AK099955 differs from that shown. Reason: Frameshift at position 407.

Ontologies

Keywords
Biological process	ER-Golgi transport Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane
Domain	Repeat WD repeat
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	COPI-coated vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane coat Inferred from electronic annotation. Source: InterPro
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 910	910	Coatomer subunit beta'-2		PRO_0000285608
Regions
	Repeat	13 – 52	40	WD 1
	Repeat	55 – 94	40	WD 2
	Repeat	97 – 136	40	WD 3
	Repeat	140 – 180	41	WD 4
	Repeat	183 – 224	42	WD 5
	Repeat	227 – 266	40	WD 6
	Repeat	269 – 309	41	WD 7
	Repeat	351 – 393	43	WD 8
	Repeat	461 – 501	41	WD 9

Sequences

	Sequence		Length	Mass (Da)
	Q6H8D5 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: FBA6B1BCE750C481	FASTA	910	102,678
	10 20 30 40 50 60 MPLRLDIKRK LAQRSERVKS VDLHPTEPWI LSSLYSGSVC IWNYQTQTMV KSFEVTELPV 70 80 90 100 110 120 RSSKFIARKQ WIVAGADDMF IRVYNYNTMD KVKVFEAHTD YIRCVAVHPT QPFVLSSSDD 130 140 150 160 170 180 MLIKLWDWDK GWMCTQIFEG HSHYVMQVTF NPKDTNTFAS ASLDRTVKVW SLGSPDPNFT 190 200 210 220 230 240 LDGHSKGVNC VDYFTGGDRP YLITGSDDQT AKVWDYQTKS CVQTLEGHAH NVSAVCFHPE 250 260 270 280 290 300 LPITLTGSED GTVRLWHSTT YRLENTLNYG LERVWALGYM KGSRRVVIGY DEGTIMIKIG 310 320 330 340 350 360 REVPVASMDS SGKIIWSKHN EIQTVNIKTI GADNEIADGE RLPLAVKELG TCDLYPQSLR 370 380 390 400 410 420 HNPNGRFVVV CGDGEYIIYT ALAWRNRSFG SALEFVWSVD GEYAVRESTS RIKIYSKNFQ 430 440 450 460 470 480 ERKSIRPPFS AERIFGGVLL AMCTNDFICF HDWAEGRMIR RIDVNVKNLY WADSGDLVTI 490 500 510 520 530 540 ASDTSFYILK YNRDVVSSHL DGGGSVGEEG VEDAFELLHE INERIRTGLW VGDCFIYNNS 550 560 570 580 590 600 SSRLNYCVGG EVTTLFHLDR QMYLLGYLAN QSRVYLIDKQ FNVVGYTLLL TMIEYKTLVM 610 620 630 640 650 660 RGDFDRANAL LPSIPKEQHD SVARFLESQG MLEEALEIAT DSNYRFDLAV QLGRLEVAKA 670 680 690 700 710 720 IAIEAQSESK WRQLGELAMS TGKLDMAEEC LLHAMDLSGL LLLYSSLGDA EGLTKLTSMA 730 740 750 760 770 780 KEQGKNNVAF LCFFMLGKLE ECLQLLIESN RIPEAALMSR SYLPSKVPEI VTLWKKDLQK 790 800 810 820 830 840 VNPKAAESLA DPDEYPNLFE DWQIALNVEA NVAPKRGIYP PAEEYIIHAE RPNETLVEAF 850 860 870 880 890 900 KSMHIHLEEV LPDENGDDTH EAIEENGVEE SQEDAVEVDV EADGSTDGAV LVNGNDTEEQ 910 WGTNNEESSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 290-910. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP003977 Genomic DNA. Translation: BAD25014.1. AP008208 Genomic DNA. Translation: BAF08178.1. AK099955 mRNA. No translation available.
RefSeq	NP_001046264.1. NM_001052799.1.
UniGene	Os.58005.
3D structure databases
HSSP	HSSP built from PDB template 1VYH based on UniProtKB P63005.
ProteinModelPortal	Q6H8D5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4328694.
KEGG	osa:4328694.
Organism-specific databases
Gramene	Q6H8D5.
Phylogenomic databases
eggNOG	COG2319.
HOGENOM	HOG000163444.
Gene expression databases
ArrayExpress	Q6H8D5.
Family and domain databases
Gene3D	1.25.40.10. 1 hit. 2.130.10.10. 2 hits.
InterPro	IPR006692. Coatomer_WD-assoc_reg. IPR016453. COPB2. IPR020472. G-protein_beta_WD-40_rep. IPR011990. TPR-like_helical. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR017986. WD40_repeat_dom. [Graphical view]
Pfam	PF04053. Coatomer_WDAD. 1 hit. PF00400. WD40. 4 hits. [Graphical view]
PIRSF	PIRSF005567. Coatomer_beta'_subunit. 1 hit.
PRINTS	PR00320. GPROTEINBRPT.
SMART	SM00320. WD40. 6 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 2 hits.
PROSITE	PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 5 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COB22_ORYSJ

Accession

Primary (citable) accession number: Q6H8D5
Secondary accession number(s): Q0E2W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program