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Protein

Bifunctional nitrilase/nitrile hydratase NIT4

Gene

NIT4

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Highly specific for beta-cyano-L-alanine (Ala(CN)). Low activity with 3-phenylpropionitrile (PPN). Not associated with auxin production but may be involved in cyanide detoxification (By similarity).By similarity

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.PROSITE-ProRule annotation
3-cyano-L-alanine + 2 H2O = L-aspartate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71Proton acceptorPROSITE-ProRule annotation1
Active sitei162Proton donorPROSITE-ProRule annotation1
Active sitei196NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional nitrilase/nitrile hydratase NIT4 (EC:3.5.5.1, EC:3.5.5.4)
Short name:
OsNIT4
Alternative name(s):
Cyanoalanine nitrilase
Nitrilase 4
Gene namesi
Name:NIT4
Ordered Locus Names:Os02g0635200, LOC_Os02g42350
ORF Names:OJ1626_B09.8, OsJ_007404
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003525131 – 362Bifunctional nitrilase/nitrile hydratase NIT4Add BLAST362

Proteomic databases

PaxDbiQ6H849.

Expressioni

Gene expression databases

GenevisibleiQ6H849. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os02g42350.1.

Structurei

3D structure databases

ProteinModelPortaliQ6H849.
SMRiQ6H849.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 307CN hydrolasePROSITE-ProRule annotationAdd BLAST277

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi255 – 258Poly-Pro4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0805. Eukaryota.
COG0388. LUCA.
HOGENOMiHOG000256365.
InParanoidiQ6H849.
KOiK13035.
OMAiTMRHIAL.
OrthoDBiEOG09360CLO.

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.
PS00921. NITRIL_CHT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6H849-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMVPSGSGG GPPVIAEVEM NGGADSGAAT VRATVVQAST VFYDTPATLD
60 70 80 90 100
KAERLIEEAA GYGSQLVVFP EAFVGGYPRG STFGFGANIS IGNPKDKGKE
110 120 130 140 150
EFRKYHAAAI EVPGPEVTRL AAMAGKYKVF LVMGVIEREG YTLYCSVLFF
160 170 180 190 200
DPLGRYLGKH RKLMPTALER IIWGFGDGST IPVYDTPLGK IGALICWENK
210 220 230 240 250
MPLLRTALYG KGIEIYCAPT ADSRQVWQAS MTHIALEGGC FVLSANQFCR
260 270 280 290 300
RKDYPPPPEY VFSGLGEEPS PDTVVCPGGS VIISPSGEVL AGPNYEGEAL
310 320 330 340 350
ITADLDLGEI VRAKFDFDVV GHYARPEVLS LVVNDQPHLP VSFTSAAEKT
360
TAAKSDSTAK PY
Length:362
Mass (Da):38,676
Last modified:July 19, 2004 - v1
Checksum:i5D3CA71FFD95FDE0
GO

Sequence cautioni

Q6H849: The sequence EAZ23921 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25D → T in BAA77679 (Ref. 1) Curated1
Sequence conflicti263S → T in BAA77679 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027054 mRNA. Translation: BAA77679.1.
AP004069 Genomic DNA. Translation: BAD25100.1.
AP008208 Genomic DNA. Translation: BAF09432.1.
AP014958 Genomic DNA. Translation: BAS79936.1.
CM000139 Genomic DNA. Translation: EAZ23921.1. Sequence problems.
AK104033 mRNA. Translation: BAG96382.1.
AK121244 mRNA. Translation: BAH00392.1.
PIRiT52266.
RefSeqiXP_015622782.1. XM_015767296.1.
UniGeneiOs.4158.

Genome annotation databases

EnsemblPlantsiOS02T0635200-01; OS02T0635200-01; OS02G0635200.
GeneIDi4330077.
GrameneiOS02T0635200-01; OS02T0635200-01; OS02G0635200.
KEGGiosa:4330077.

Similar proteinsi

Entry informationi

Entry nameiNRL4_ORYSJ
AccessioniPrimary (citable) accession number: Q6H849
Secondary accession number(s): A3A9D2, B7EVI5, Q9SXX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: July 19, 2004
Last modified: October 25, 2017
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families