ID   P2C23_ORYSJ             Reviewed;         319 AA.
AC   Q6H7J4; A0A0P0VM21;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   27-MAR-2024, entry version 90.
DE   RecName: Full=Putative protein phosphatase 2C 23;
DE            Short=OsPP2C23;
DE            EC=3.1.3.16;
GN   OrderedLocusNames=Os02g0633700, LOC_Os02g42250;
GN   ORFNames=OJ1643_A10.22;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=19021904; DOI=10.1186/1471-2164-9-550;
RA   Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y.;
RT   "Genome-wide and expression analysis of protein phosphatase 2C in rice and
RT   Arabidopsis.";
RL   BMC Genomics 9:550-550(2008).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC         COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:83421; EC=3.1.3.16;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC         phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC         COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:61977; EC=3.1.3.16;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Binds 2 magnesium ions per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the PP2C family. {ECO:0000305}.
CC   -!- CAUTION: Although related to the protein phosphatase 2C family, lacks 1
CC       of the conserved residues that bind manganese, suggesting it has no
CC       phosphatase activity. {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. {ECO:0000305}.
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DR   EMBL; AP004192; BAD25305.1; -; Genomic_DNA.
DR   EMBL; AP014958; BAS79925.1; -; Genomic_DNA.
DR   RefSeq; XP_015624508.1; XM_015769022.1.
DR   AlphaFoldDB; Q6H7J4; -.
DR   SMR; Q6H7J4; -.
DR   STRING; 39947.Q6H7J4; -.
DR   PaxDb; 39947-Q6H7J4; -.
DR   EnsemblPlants; Os02t0633700-00; Os02t0633700-00; Os02g0633700.
DR   GeneID; 107275989; -.
DR   Gramene; Os02t0633700-00; Os02t0633700-00; Os02g0633700.
DR   KEGG; osa:107275989; -.
DR   eggNOG; KOG1379; Eukaryota.
DR   HOGENOM; CLU_029404_3_1_1; -.
DR   InParanoid; Q6H7J4; -.
DR   OMA; ANPATCG; -.
DR   OrthoDB; 377083at2759; -.
DR   Proteomes; UP000000763; Chromosome 2.
DR   Proteomes; UP000059680; Chromosome 2.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004722; F:protein serine/threonine phosphatase activity; IBA:GO_Central.
DR   Gene3D; 3.60.40.10; PPM-type phosphatase domain; 1.
DR   InterPro; IPR036457; PPM-type-like_dom_sf.
DR   InterPro; IPR001932; PPM-type_phosphatase-like_dom.
DR   InterPro; IPR039123; PPTC7.
DR   PANTHER; PTHR12320; PROTEIN PHOSPHATASE 2C; 1.
DR   PANTHER; PTHR12320:SF84; PROTEIN PHOSPHATASE 2C 23-RELATED; 1.
DR   SMART; SM00332; PP2Cc; 1.
DR   SUPFAM; SSF81606; PP2C-like; 1.
DR   PROSITE; PS51746; PPM_2; 1.
DR   Genevisible; Q6H7J4; OS.
PE   5: Uncertain;
KW   Hydrolase; Magnesium; Manganese; Metal-binding; Protein phosphatase;
KW   Reference proteome.
FT   CHAIN           1..319
FT                   /note="Putative protein phosphatase 2C 23"
FT                   /id="PRO_0000363269"
FT   DOMAIN          73..314
FT                   /note="PPM-type phosphatase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01082"
FT   BINDING         102
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         235
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         305
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   319 AA;  34728 MW;  B35E3988B0F66490 CRC64;
     MEKRMETLEQ IKETLRETSK LVPDIVRAAV GLEHHYQTVE LPHDDGCVKS FAAAFLRPQA
     QEQAHGDGEV QQAVRMESAS CYVPDHDEDA HFVHDAAGVV GGYRRRVGVD AGAFSRGLMT
     SAFAQLVTAE PGTPVCPYTL LERAYEETLE SGAQGGSTAV ILSLADGNVL RWAYIGDSAF
     AVLRDGRVVV RSVQQQRYFN APYYLGGRRG DEGMTVGMVG EMKVRRGDVV VAGTDGLFDN
     MSDAELEKVV QIGTALGFSP KNMADIIGGT AYEMSRCLLK DSPFAVEWRK QHENEEGHFY
     GGKVDDITVV VACIVSSDS
//