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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] 2, chloroplastic

Gene

Os09g0277800

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the NAD-dependent reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Catalyzes the last reduction step in the de novo synthesis cycle of fatty acids. Involved in the elongation cycle of fatty acids which are used in lipid metabolism. Required for normal plant growth (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87NAD; via carbonyl oxygenBy similarity1
Binding sitei94NADBy similarity1
Binding sitei248NAD; via carbonyl oxygenBy similarity1
Active sitei250Proton acceptorBy similarity1
Active sitei260Proton acceptorBy similarity1
Binding sitei268NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi151 – 152NADBy similarity2
Nucleotide bindingi198 – 199NADBy similarity2
Nucleotide bindingi298 – 302NADBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] 2, chloroplastic (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Ordered Locus Names:Os09g0277800, LOC_Os09g10600
ORF Names:P0701E06.10
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryzaOryza sativa
Proteomesi
  • UP000059680 Componenti: Chromosome 9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 67ChloroplastSequence analysisAdd BLAST67
ChainiPRO_000042028068 – 371Enoyl-[acyl-carrier-protein] reductase [NADH] 2, chloroplasticAdd BLAST304

Proteomic databases

PaxDbiQ6H5J0.
PRIDEiQ6H5J0.

Expressioni

Gene expression databases

GenevisibleiQ6H5J0. OS.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os09g10600.1.

Structurei

3D structure databases

ProteinModelPortaliQ6H5J0.
SMRiQ6H5J0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IQSQ. Eukaryota.
COG0623. LUCA.
InParanoidiQ6H5J0.
KOiK00208.
OMAiNHSIAWG.
OrthoDBiEOG09360GOD.

Family and domain databases

InterProiView protein in InterPro
IPR036291. NAD(P)-bd_dom_sf.
IPR002347. SDR_fam.
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6H5J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGASVTTGLQ MAAARPCIPA CQRLLGSRAA LPSFGRALST QTGFASCRKT
60 70 80 90 100
ASAGPFVSLN HKRFAVRAMS AQGLPIDLRG KRAFIAGVAD DNGYGWAIAK
110 120 130 140 150
ALAAAGAEIL VGTWVPALNI FETSLRRGKF DESRKLPDGS LMEITKVYPL
160 170 180 190 200
DAVFDSPEDV PDDVKANKRY AGSSNWTVKE VAETVKNDFG TIDILVHSLA
210 220 230 240 250
NGPEVKNSLL ETSRKGYLAA VSASSYSFIS LLQHFLPIMN PGGATISLTY
260 270 280 290 300
IASERTIPGY GGGMSSAKAA LESDTRVLAY EAGRKGKIRV NTISAGPLGS
310 320 330 340 350
RAAKAIGFIE KMIEYSYVNA PLQKELLADE VGNTAAFLAS PLASAITGST
360 370
IYVDNGLNTM GLALDSPTLS T
Length:371
Mass (Da):39,021
Last modified:July 19, 2004 - v1
Checksum:i7AEE27D8CBC35E68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP005594 Genomic DNA. Translation: BAD26009.1.
AP008215 Genomic DNA. Translation: BAF24666.1.
AP014965 Genomic DNA. Translation: BAT07201.1.
AK103141 mRNA. Translation: BAG95916.1.
RefSeqiXP_015610600.1. XM_015755114.1.
UniGeneiOs.27167.

Genome annotation databases

EnsemblPlantsiOS09T0277800-01; OS09T0277800-01; OS09G0277800.
GeneIDi4346596.
GrameneiOS09T0277800-01; OS09T0277800-01; OS09G0277800.
KEGGiosa:4346596.

Similar proteinsi

Entry informationi

Entry nameiFABI2_ORYSJ
AccessioniPrimary (citable) accession number: Q6H5J0
Secondary accession number(s): A0A0P0XJW8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: July 19, 2004
Last modified: October 25, 2017
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families