true2004-12-212024-01-24152ULBP5_HUMANTwo human ULBP/RAET1 molecules with transmembrane regions are ligands for NKG2D.Bacon L.Eagle R.A.Meyer M.Easom N.Young N.T.Trowsdale J.doi:10.4049/jimmunol.173.2.10782004J. Immunol.1731078-1084NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2)FUNCTIONSUBCELLULAR LOCATIONTISSUE SPECIFICITYINTERACTION WITH KLRK1 AND CMV UL16Four novel ULBP splice variants are ligands for human NKG2D.Cao W.Xi X.Wang Z.Dong L.Hao Z.Cui L.Ma C.He W.doi:10.1093/intimm/dxn0572008Int. Immunol.20981-991ALTERNATIVE SPLICING (ISOFORM 3)INTERACTION WITH KLRK1ULBP6/RAET1L is an additional human NKG2D ligand.Eagle R.A.Traherne J.A.Hair J.R.Jafferji I.Trowsdale J.doi:10.1002/eji.2009395022009Eur. J. Immunol.393207-3216FUNCTIONINTERACTION WITH KLRK1 AND CMV UL16TISSUE SPECIFICITYMass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.Wollscheid B.Bausch-Fluck D.Henderson C.O'Brien R.Bibel M.Schiess R.Aebersold R.Watts J.D.doi:10.1038/nbt.15322009Nat. Biotechnol.27378-386GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-82Leukemic T-cellCellular expression, trafficking, and function of two isoforms of human ULBP5/RAET1G.Eagle R.A.Flack G.Warford A.Martinez-Borra J.Jafferji I.Traherne J.A.Ohashi M.Boyle L.H.Barrow A.D.Caillat-Zucman S.Young N.T.Trowsdale J.doi:10.1371/journal.pone.00045032009PLoS ONE4E4503SUBCELLULAR LOCATION (ISOFORMS 1 AND 2)TISSUE SPECIFICITYPost-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.Ohashi M.Eagle R.A.Trowsdale J.doi:10.1074/jbc.m109.0776362010J. Biol. Chem.28516408-16415SUBCELLULAR LOCATIONGPI-ANCHOR AT GLY-218PROTEOLYTIC PROCESSINGMUTAGENESIS OF SER-217121 site, No reported glycans1 site33 antibodies from 8 providershumanRAET1GTissue enhanced (adrenal gland, esophagus)geneEukaryotaPost-translational modification: synthesis of GPI-anchored proteins13 hits in 1105 CRISPR screensTbioProteinExpressed in esophagus mucosa and 90 other cell types or tissuesbaseline and differentialMHC class I-like antigen recognition-likeMHC_I-like_Ag-recogMHC_I-like_Ag-recog_sfMHC_I/II-like_Ag-recogMHC CLASS I-RELATEDUL-16 BINDING PROTEIN 5MHC_IMHC antigen-recognition domainUL-16 binding protein 5Retinoic acid early transcript 1G proteinRAET1GULBP5Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.Down-regulates the expression of KLRK1 and stimulates natural killer cells to secrete IFNG.Stimulates natural killer cells to secrete IFNG.Interacts with KLRK1/NKG2D.(Microbial infection) In CMV-infected cells, interacts with the viral glycoprotein UL16; this interaction causes RAET1G retention in the endoplasmic reticulum and cis-Golgi and prevents binding to and activation of KLRK1/NKG2D, providing CMV with an immune evasion mechanism.Mainly found intracellularly.Isoform 1 is highly expressed in colon and in a number of tumor cell lines and highly restricted in normal tissues. Both isoforms are frequently expressed in cell lines derived from epithelial cancers, and in primary breast cancers.The functional form is cleaved C-terminally of the GPI-anchor and yields a 28 kDa protein.UL16-binding proteins (ULBPs) are unusual members of the extended MHC class I superfamily. They do not contain the alpha 3 domain and lack a transmembrane domain.Belongs to the MHC class I family.125UL-16 binding protein 52165826218Removed in mature form219334Extracellular223Helical224243Cytoplasmic244MHC class I alpha-1 like29117MHC class I alpha-2 like118210GPI-anchor amidated glycineN-linked (GlcNAc...) asparagine825066127190In isoform 3.154In isoform 2.GTV211213In isoform 2 and isoform 3.214R70Reduced cell surface expression.P217false6KLRK12004-07-191true37106b0d8affafec05fab6fd2ac42a3128d761RAET1G1MAAAASPAFLLRLPLLLLLSSWCRTGLADPHSLCYDITVIPKFRPGPRWCAVQGQVDEKTFLHYDCGSKTVTPVSPLGKKLNVTTAWKAQNPVLREVVDILTEQLLDIQLENYIPKEPLTLQARMSCEQKAEGHGSGSWQLSFDGQIFLLFDSENRMWTTVHPGARKMKEKWENDKDMTMSFHYISMGDCTGWLEDFLMGMDSTLEPSAGAPPTMSSGTAQPRATATTLILCCLLIMCLLICSRHSLTQSHGHHPQSLQPPPHPPLLHPTWLLRRVLWSDSYQIAKRPLSGGHVTRVTLPIIGDDSHSLPCPLALYTINNGAARYSEPLQVSIS2RAET1G2MAAAASPAFLLRLPLLLLLSSWCRTGLADPHSLCYDITVIPKFRPGPRWCAVQGQVDEKTFLHYDCGSKTVTPVSPLGKKLNVTTAWKAQNPVLREVVDILTEQLLDIQLENYIPKEPLTLQARMSCEQKAEGHGSGSWQLSFDGQIFLLFDSENRMWTTVHPGARKMKEKWENDKDMTMSFHYISMGDCTGWLEDFLMGMDSTLEPSAGGTV3RAET1G3MAAAASPAFLLRLPLLLLLSSWCRTGLADPHSLCYDITVIPKFRPGPRWCAVQGQVDEKTFLHYDCGSKTVTPVSPLGKKLNVTTAWKAQNPVLREVVDILTEQLLDIQLENYIPKENRMWTTVHPGARKMKEKWENDKDMTMSFHYISMGDCTGWLEDFLMGMDSTLEPSAGAPPtruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetruetrue