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Q6H3X3 (RET1G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Retinoic acid early transcript 1G protein
Alternative name(s):
UL-16 binding protein 5
Short name=ULBP5
Gene names
Name:RAET1G
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length334 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a ligand for the KLRK1 receptor and mediates NK cell cytotoxicity via the receptor. Ref.1

Subunit structure

Binds to KLRK1/NKG2D. Also binds the CMV glycoprotein UL16. Ref.1

Subcellular location

Isoform 1: Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchorGPI-anchor. Endoplasmic reticulum. Note: Mainly found inside the cell, with a minority of the protein reaching the cell surface. Ref.1 Ref.3 Ref.4

Isoform 2: Secreted Ref.1 Ref.3 Ref.4.

Tissue specificity

Isoform 1 is highly expressed in colon and in a number of tumor cell lines and highly restricted in normal tissues. Both isoforms are frequently expressed in cell lines derived from epithelial cancers, and in primary breast cancers. Ref.1 Ref.3

Post-translational modification

The functional form is cleaved C-terminally of the GPI-anchor and yields a 28 kDa protein. Ref.4

Sequence similarities

Belongs to the MHC class I family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q6H3X3-1)

Also known as: RAET1G1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.1 (identifier: Q6H3X3-2)

Also known as: RAET1G2;

The sequence of this isoform differs from the canonical sequence as follows:
     211-213: APP → GTV
     214-334: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 By similarity UniProtKB Q9BZM5
Chain26 – 218193Retinoic acid early transcript 1G protein
PRO_0000019022
Propeptide219 – 334116Removed in mature form Potential
PRO_0000429932

Regions

Topological domain26 – 223198Extracellular Potential
Transmembrane224 – 24320Helical; Potential
Topological domain244 – 33491Cytoplasmic Potential
Region29 – 11789MHC class I alpha-1 like
Region118 – 21093MHC class I alpha-2 like

Amino acid modifications

Lipidation2181GPI-anchor amidated glycine Probable
Glycosylation821N-linked (GlcNAc...) Ref.2
Disulfide bond50 ↔ 66 By similarity UniProtKB Q9BZM4
Disulfide bond127 ↔ 190 By similarity UniProtKB Q9BZM4

Natural variations

Alternative sequence211 – 2133APP → GTV in isoform 2. Ref.1
VSP_051621
Alternative sequence214 – 334121Missing in isoform 2. Ref.1
VSP_051622
Natural variant701T → R.
Corresponds to variant rs9397449 [ dbSNP | Ensembl ].
VAR_033449

Experimental info

Mutagenesis2171S → P: Reduced cell surface expression. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (RAET1G1) [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 3F8350036951BA69

FASTA33437,106
        10         20         30         40         50         60 
MAAAASPAFL LRLPLLLLLS SWCRTGLADP HSLCYDITVI PKFRPGPRWC AVQGQVDEKT 

        70         80         90        100        110        120 
FLHYDCGSKT VTPVSPLGKK LNVTTAWKAQ NPVLREVVDI LTEQLLDIQL ENYIPKEPLT 

       130        140        150        160        170        180 
LQARMSCEQK AEGHGSGSWQ LSFDGQIFLL FDSENRMWTT VHPGARKMKE KWENDKDMTM 

       190        200        210        220        230        240 
SFHYISMGDC TGWLEDFLMG MDSTLEPSAG APPTMSSGTA QPRATATTLI LCCLLIMCLL 

       250        260        270        280        290        300 
ICSRHSLTQS HGHHPQSLQP PPHPPLLHPT WLLRRVLWSD SYQIAKRPLS GGHVTRVTLP 

       310        320        330 
IIGDDSHSLP CPLALYTINN GAARYSEPLQ VSIS 

« Hide

Isoform 2 (RAET1G2) [UniParc].

Checksum: 24E4AC61CE0F8854
Show »

FASTA21323,884

References

« Hide 'large scale' references
[1]"Two human ULBP/RAET1 molecules with transmembrane regions are ligands for NKG2D."
Bacon L., Eagle R.A., Meyer M., Easom N., Young N.T., Trowsdale J.
J. Immunol. 173:1078-1084(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH KLRK1 AND UL16.
[2]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-82.
Tissue: Leukemic T-cell.
[3]"Cellular expression, trafficking, and function of two isoforms of human ULBP5/RAET1G."
Eagle R.A., Flack G., Warford A., Martinez-Borra J., Jafferji I., Traherne J.A., Ohashi M., Boyle L.H., Barrow A.D., Caillat-Zucman S., Young N.T., Trowsdale J.
PLoS ONE 4:E4503-E4503(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
[4]"Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform."
Ohashi M., Eagle R.A., Trowsdale J.
J. Biol. Chem. 285:16408-16415(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, GPI-ANCHOR, PROTEOLYTIC PROCESSING, MUTAGENESIS OF SER-217.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY172579 mRNA. Translation: AAO22238.1.
AY172580 mRNA. Translation: AAO22239.1.
CCDSCCDS43514.1. [Q6H3X3-1]
RefSeqNP_001001788.2. NM_001001788.2. [Q6H3X3-1]
UniGeneHs.558792.

3D structure databases

ProteinModelPortalQ6H3X3.
SMRQ6H3X3. Positions 29-235.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ6H3X3. 1 interaction.
STRING9606.ENSP00000356329.

PTM databases

PhosphoSiteQ6H3X3.

Polymorphism databases

DMDM56749591.

Proteomic databases

PaxDbQ6H3X3.
PRIDEQ6H3X3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000367360; ENSP00000356329; ENSG00000203722. [Q6H3X3-1]
ENST00000367361; ENSP00000356330; ENSG00000203722. [Q6H3X3-2]
GeneID353091.
KEGGhsa:353091.
UCSCuc010kii.1. human. [Q6H3X3-1]

Organism-specific databases

CTD353091.
GeneCardsGC06M150238.
H-InvDBHIX0025204.
HGNCHGNC:16795. RAET1G.
MIM609244. gene.
neXtProtNX_Q6H3X3.
PharmGKBPA134897742.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG145571.
HOGENOMHOG000070028.
InParanoidQ6H3X3.
KOK07987.
OMAGAARYSE.
OrthoDBEOG7HB59Z.
PhylomeDBQ6H3X3.
TreeFamTF341724.

Gene expression databases

ArrayExpressQ6H3X3.
BgeeQ6H3X3.
GenevestigatorQ6H3X3.

Family and domain databases

Gene3D3.30.500.10. 1 hit.
InterProIPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]
PfamPF00129. MHC_I. 1 hit.
[Graphical view]
SUPFAMSSF54452. SSF54452. 1 hit.
ProtoNetSearch...

Other

GenomeRNAi353091.
NextBio99647.
PROQ6H3X3.
SOURCESearch...

Entry information

Entry nameRET1G_HUMAN
AccessionPrimary (citable) accession number: Q6H3X3
Secondary accession number(s): Q6H3X2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: July 19, 2004
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM