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Q6H3X3

- RET1G_HUMAN

UniProt

Q6H3X3 - RET1G_HUMAN

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Protein

Retinoic acid early transcript 1G protein

Gene
RAET1G
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a ligand for the KLRK1 receptor and mediates NK cell cytotoxicity via the receptor.1 Publication

GO - Molecular functioni

  1. antigen binding Source: RefGenome
  2. natural killer cell lectin-like receptor binding Source: UniProtKB

GO - Biological processi

  1. antigen processing and presentation Source: RefGenome
  2. natural killer cell mediated cytotoxicity Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Retinoic acid early transcript 1G protein
Alternative name(s):
UL-16 binding protein 5
Short name:
ULBP5
Gene namesi
Name:RAET1G
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 6

Organism-specific databases

HGNCiHGNC:16795. RAET1G.

Subcellular locationi

Isoform 1 : Cell membrane; Single-pass type I membrane protein. Cell membrane; Lipid-anchorGPI-anchor. Endoplasmic reticulum
Note: Mainly found inside the cell, with a minority of the protein reaching the cell surface.3 Publications
Isoform 2 : Secreted 3 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini26 – 223198Extracellular Reviewed predictionAdd
BLAST
Transmembranei224 – 24320Helical; Reviewed predictionAdd
BLAST
Topological domaini244 – 33491Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endoplasmic reticulum, Membrane, Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi217 – 2171S → P: Reduced cell surface expression. 1 Publication

Organism-specific databases

PharmGKBiPA134897742.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525 By similarityBy similarityAdd
BLAST
Chaini26 – 218193Retinoic acid early transcript 1G proteinPRO_0000019022Add
BLAST
Propeptidei219 – 334116Removed in mature form Reviewed predictionPRO_0000429932Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 66 By similarityBy similarity
Glycosylationi82 – 821N-linked (GlcNAc...)1 Publication
Disulfide bondi127 ↔ 190 By similarityBy similarity
Lipidationi218 – 2181GPI-anchor amidated glycine Inferred

Post-translational modificationi

The functional form is cleaved C-terminally of the GPI-anchor and yields a 28 kDa protein.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ6H3X3.
PRIDEiQ6H3X3.

PTM databases

PhosphoSiteiQ6H3X3.

Expressioni

Tissue specificityi

Isoform 1 is highly expressed in colon and in a number of tumor cell lines and highly restricted in normal tissues. Both isoforms are frequently expressed in cell lines derived from epithelial cancers, and in primary breast cancers.2 Publications

Gene expression databases

ArrayExpressiQ6H3X3.
BgeeiQ6H3X3.
GenevestigatoriQ6H3X3.

Interactioni

Subunit structurei

Binds to KLRK1/NKG2D. Also binds the CMV glycoprotein UL16.1 Publication

Protein-protein interaction databases

IntActiQ6H3X3. 1 interaction.
STRINGi9606.ENSP00000356329.

Structurei

3D structure databases

ProteinModelPortaliQ6H3X3.
SMRiQ6H3X3. Positions 29-235.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 11789MHC class I alpha-1 likeAdd
BLAST
Regioni118 – 21093MHC class I alpha-2 likeAdd
BLAST

Sequence similaritiesi

Belongs to the MHC class I family.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG145571.
HOGENOMiHOG000070028.
InParanoidiQ6H3X3.
KOiK07987.
OMAiGAARYSE.
OrthoDBiEOG7HB59Z.
PhylomeDBiQ6H3X3.
TreeFamiTF341724.

Family and domain databases

Gene3Di3.30.500.10. 1 hit.
InterProiIPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
[Graphical view]
PfamiPF00129. MHC_I. 1 hit.
[Graphical view]
SUPFAMiSSF54452. SSF54452. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q6H3X3-1) [UniParc]FASTAAdd to Basket

Also known as: RAET1G1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAAAASPAFL LRLPLLLLLS SWCRTGLADP HSLCYDITVI PKFRPGPRWC    50
AVQGQVDEKT FLHYDCGSKT VTPVSPLGKK LNVTTAWKAQ NPVLREVVDI 100
LTEQLLDIQL ENYIPKEPLT LQARMSCEQK AEGHGSGSWQ LSFDGQIFLL 150
FDSENRMWTT VHPGARKMKE KWENDKDMTM SFHYISMGDC TGWLEDFLMG 200
MDSTLEPSAG APPTMSSGTA QPRATATTLI LCCLLIMCLL ICSRHSLTQS 250
HGHHPQSLQP PPHPPLLHPT WLLRRVLWSD SYQIAKRPLS GGHVTRVTLP 300
IIGDDSHSLP CPLALYTINN GAARYSEPLQ VSIS 334
Length:334
Mass (Da):37,106
Last modified:July 19, 2004 - v1
Checksum:i3F8350036951BA69
GO
Isoform 21 Publication (identifier: Q6H3X3-2) [UniParc]FASTAAdd to Basket

Also known as: RAET1G2

The sequence of this isoform differs from the canonical sequence as follows:
     211-213: APP → GTV
     214-334: Missing.

Show »
Length:213
Mass (Da):23,884
Checksum:i24E4AC61CE0F8854
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti70 – 701T → R.
Corresponds to variant rs9397449 [ dbSNP | Ensembl ].
VAR_033449

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei211 – 2133APP → GTV in isoform 2. 1 PublicationVSP_051621
Alternative sequencei214 – 334121Missing in isoform 2. 1 PublicationVSP_051622Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY172579 mRNA. Translation: AAO22238.1.
AY172580 mRNA. Translation: AAO22239.1.
CCDSiCCDS43514.1. [Q6H3X3-1]
RefSeqiNP_001001788.2. NM_001001788.2. [Q6H3X3-1]
UniGeneiHs.558792.

Genome annotation databases

EnsembliENST00000367360; ENSP00000356329; ENSG00000203722. [Q6H3X3-1]
ENST00000367361; ENSP00000356330; ENSG00000203722. [Q6H3X3-2]
GeneIDi353091.
KEGGihsa:353091.
UCSCiuc010kii.1. human. [Q6H3X3-1]

Polymorphism databases

DMDMi56749591.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY172579 mRNA. Translation: AAO22238.1 .
AY172580 mRNA. Translation: AAO22239.1 .
CCDSi CCDS43514.1. [Q6H3X3-1 ]
RefSeqi NP_001001788.2. NM_001001788.2. [Q6H3X3-1 ]
UniGenei Hs.558792.

3D structure databases

ProteinModelPortali Q6H3X3.
SMRi Q6H3X3. Positions 29-235.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q6H3X3. 1 interaction.
STRINGi 9606.ENSP00000356329.

PTM databases

PhosphoSitei Q6H3X3.

Polymorphism databases

DMDMi 56749591.

Proteomic databases

PaxDbi Q6H3X3.
PRIDEi Q6H3X3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000367360 ; ENSP00000356329 ; ENSG00000203722 . [Q6H3X3-1 ]
ENST00000367361 ; ENSP00000356330 ; ENSG00000203722 . [Q6H3X3-2 ]
GeneIDi 353091.
KEGGi hsa:353091.
UCSCi uc010kii.1. human. [Q6H3X3-1 ]

Organism-specific databases

CTDi 353091.
GeneCardsi GC06M150238.
H-InvDB HIX0025204.
HGNCi HGNC:16795. RAET1G.
MIMi 609244. gene.
neXtProti NX_Q6H3X3.
PharmGKBi PA134897742.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG145571.
HOGENOMi HOG000070028.
InParanoidi Q6H3X3.
KOi K07987.
OMAi GAARYSE.
OrthoDBi EOG7HB59Z.
PhylomeDBi Q6H3X3.
TreeFami TF341724.

Miscellaneous databases

GenomeRNAii 353091.
NextBioi 99647.
PROi Q6H3X3.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q6H3X3.
Bgeei Q6H3X3.
Genevestigatori Q6H3X3.

Family and domain databases

Gene3Di 3.30.500.10. 1 hit.
InterProi IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
[Graphical view ]
Pfami PF00129. MHC_I. 1 hit.
[Graphical view ]
SUPFAMi SSF54452. SSF54452. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two human ULBP/RAET1 molecules with transmembrane regions are ligands for NKG2D."
    Bacon L., Eagle R.A., Meyer M., Easom N., Young N.T., Trowsdale J.
    J. Immunol. 173:1078-1084(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH KLRK1 AND UL16.
  2. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-82.
    Tissue: Leukemic T-cell.
  3. Cited for: SUBCELLULAR LOCATION (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
  4. "Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform."
    Ohashi M., Eagle R.A., Trowsdale J.
    J. Biol. Chem. 285:16408-16415(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, GPI-ANCHOR, PROTEOLYTIC PROCESSING, MUTAGENESIS OF SER-217.

Entry informationi

Entry nameiRET1G_HUMAN
AccessioniPrimary (citable) accession number: Q6H3X3
Secondary accession number(s): Q6H3X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: July 19, 2004
Last modified: July 9, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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