Q6H3X3 (RET1G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retinoic acid early transcript 1G protein

Gene names

Name:

RAET1G

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a ligand for the NKG2D receptor and mediates NK cell cytotoxicity via the receptor. Ref.1
Subunit structure	Binds to KLRK1/NKG2D. Also binds the CMV glycoprotein UL16. Ref.1
Subcellular location	Cell membrane; Single-pass type I membrane protein Ref.1.
Tissue specificity	Highly expressed in colon and in a number of tumor cell lines. Ref.1
Sequence similarities	Belongs to the MHC class I family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation Inferred from electronic annotation. Source: InterPro natural killer cell mediated cytotoxicity Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	MHC class I protein complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Non-traceable author statement Ref.1. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q6H3X3-1)

Also known as: RAET1G1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: Q6H3X3-2)

Also known as: RAET1G2;

The sequence of this isoform differs from the canonical sequence as follows:
     211-213: APP → GTV
     214-334: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	By similarity UniProtKB Q9BZM5
	Chain	26 – 334	309	Retinoic acid early transcript 1G protein UniProtKB Q9BZM5		PRO_0000019022
Regions
	Topological domain	26 – 223	198	Extracellular Potential
	Transmembrane	224 – 243	20	Helical; Potential
	Topological domain	244 – 334	91	Cytoplasmic Potential
	Region	29 – 117	89	MHC class I alpha-1 like
	Region	118 – 210	93	MHC class I alpha-2 like
Amino acid modifications
	Glycosylation	82	1	N-linked (GlcNAc...) Ref.2
	Disulfide bond	50 ↔ 66		By similarity UniProtKB Q9BZM4
	Disulfide bond	127 ↔ 190		By similarity UniProtKB Q9BZM4
Natural variations
	Alternative sequence	211 – 213	3	APP → GTV in isoform 2. Ref.1		VSP_051621
	Alternative sequence	214 – 334	121	Missing in isoform 2. Ref.1		VSP_051622
	Natural variant	70	1	T → R. Corresponds to variant rs9397449 [ dbSNP | Ensembl ].		VAR_033449

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (RAET1G1) [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 3F8350036951BA69	FASTA	334	37,106
	10 20 30 40 50 60 MAAAASPAFL LRLPLLLLLS SWCRTGLADP HSLCYDITVI PKFRPGPRWC AVQGQVDEKT 70 80 90 100 110 120 FLHYDCGSKT VTPVSPLGKK LNVTTAWKAQ NPVLREVVDI LTEQLLDIQL ENYIPKEPLT 130 140 150 160 170 180 LQARMSCEQK AEGHGSGSWQ LSFDGQIFLL FDSENRMWTT VHPGARKMKE KWENDKDMTM 190 200 210 220 230 240 SFHYISMGDC TGWLEDFLMG MDSTLEPSAG APPTMSSGTA QPRATATTLI LCCLLIMCLL 250 260 270 280 290 300 ICSRHSLTQS HGHHPQSLQP PPHPPLLHPT WLLRRVLWSD SYQIAKRPLS GGHVTRVTLP 310 320 330 IIGDDSHSLP CPLALYTINN GAARYSEPLQ VSIS
	Isoform 2 (RAET1G2) [UniParc]. Checksum: 24E4AC61CE0F8854	FASTA	213	23,884
	10 20 30 40 50 60 MAAAASPAFL LRLPLLLLLS SWCRTGLADP HSLCYDITVI PKFRPGPRWC AVQGQVDEKT 70 80 90 100 110 120 FLHYDCGSKT VTPVSPLGKK LNVTTAWKAQ NPVLREVVDI LTEQLLDIQL ENYIPKEPLT 130 140 150 160 170 180 LQARMSCEQK AEGHGSGSWQ LSFDGQIFLL FDSENRMWTT VHPGARKMKE KWENDKDMTM 190 200 210 SFHYISMGDC TGWLEDFLMG MDSTLEPSAG GTV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two human ULBP/RAET1 molecules with transmembrane regions are ligands for NKG2D." Bacon L., Eagle R.A., Meyer M., Easom N., Young N.T., Trowsdale J. J. Immunol. 173:1078-1084(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH KLRK1 AND UL16.
[2]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-82, MASS SPECTROMETRY. Tissue: Leukemic T-cell.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY172579 mRNA. Translation: AAO22238.1. AY172580 mRNA. Translation: AAO22239.1.
IPI	IPI00433027. IPI00477175.
RefSeq	NP_001001788.2. NM_001001788.2.
UniGene	Hs.558792.
3D structure databases
ProteinModelPortal	Q6H3X3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q6H3X3. 1 interaction.
STRING	9606.ENSP00000356329.
PTM databases
PhosphoSite	Q6H3X3.
Polymorphism databases
DMDM	56749591.
Proteomic databases
PaxDb	Q6H3X3.
PRIDE	Q6H3X3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000367360; ENSP00000356329; ENSG00000203722. ENST00000367361; ENSP00000356330; ENSG00000203722.
GeneID	353091.
KEGG	hsa:353091.
UCSC	uc010kii.1. human.
Organism-specific databases
CTD	353091.
GeneCards	GC06M150238.
H-InvDB	HIX0025204.
HGNC	HGNC:16795. RAET1G.
MIM	609244. gene.
neXtProt	NX_Q6H3X3.
PharmGKB	PA134897742.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG145571.
HOGENOM	HOG000070028.
InParanoid	Q6H3X3.
KO	K07987.
OMA	GAARYSE.
OrthoDB	EOG4FBHTV.
Gene expression databases
ArrayExpress	Q6H3X3.
Bgee	Q6H3X3.
Genevestigator	Q6H3X3.
GermOnline	ENSG00000203722. Homo sapiens.
Family and domain databases
Gene3D	3.30.500.10. 1 hit.
InterPro	IPR011161. MHC_I-like_Ag-recog. IPR011162. MHC_I/II-like_Ag-recog. IPR001039. MHC_I_a_a1/a2. [Graphical view]
Pfam	PF00129. MHC_I. 1 hit. [Graphical view]
SUPFAM	SSF54452. MHC_I/II-like_Ag-recog. 1 hit.
PROSITE	PS00290. IG_MHC. False negative. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	353091.
NextBio	99647.
SOURCE	Search...

Entry information

Entry name

RET1G_HUMAN

Accession

Primary (citable) accession number: Q6H3X3
Secondary accession number(s): Q6H3X2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.