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Q6H321 (KLK2_HORSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kallikrein-1E2

EC=3.4.21.35
Alternative name(s):
Glandular kallikrein
HPK
Gene names
Name:KLK1E2
OrganismEquus caballus (Horse) [Reference proteome]
Taxonomic identifier9796 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus

Protein attributes

Sequence length261 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activity

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Subcellular location

Secreted Ref.2.

Tissue specificity

Detected in prostate and semen. Ref.2

Sequence similarities

Belongs to the peptidase S1 family. Kallikrein subfamily.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Probable
Propeptide18 – 247Activation peptide Probable
PRO_0000027921
Chain25 – 261237Kallikrein-1E2
PRO_0000027922

Regions

Domain25 – 258234Peptidase S1

Sites

Active site651Charge relay system By similarity
Active site1201Charge relay system By similarity
Active site2131Charge relay system By similarity

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Potential
Disulfide bond31 ↔ 173
Disulfide bond50 ↔ 66
Disulfide bond152 ↔ 219
Disulfide bond184 ↔ 198
Disulfide bond209 ↔ 234

Experimental info

Sequence conflict821E → K in CAD20985. Ref.2
Sequence conflict2501L → K Ref.2

Secondary structure

............................................. 261
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q6H321 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: DD612F74EAE0CE49

FASTA26129,392
        10         20         30         40         50         60 
MWFLVLCLDL SLGETGALPP IQSRIIGGWE CEKHSKPWQV AVYHQGHFQC GGVLVHPQWV 

        70         80         90        100        110        120 
LTAAHCMSDD YQIWLGRHNL SEDEDTAQFH QVSDSFLDPQ FDLSLLKKKY LRPYDDISHD 

       130        140        150        160        170        180 
LMLLRLAQPA RITDAVKILD LPTQEPKLGS TCYTSGWGLI STFTNRGSGT LQCVELRLQS 

       190        200        210        220        230        240 
NEKCARAYPE KMTEFVLCAT HRDDSGSICL GDSGGALICD GVFQGITSWG YSECADFNDN 

       250        260 
FVFTKVMPHL KWIKETIEKN S 

« Hide

References

[1]"Taxon-specific evolution of glandular kallikrein genes and identification of a progenitor of prostate-specific antigen."
Olsson A.Y., Lilja H., Lundwall A.
Genomics 84:147-156(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Prostate.
[2]"Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: a homologue of human PSA."
Carvalho A.L., Sanz L., Barettino D., Romero A., Calvete J.J., Romao M.J.
J. Mol. Biol. 322:325-337(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 62-252, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, X-RAY CRYSTALLOGRAPHY (1.42 ANGSTROMS) OF 25-261.
Tissue: Prostate.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY290704 mRNA. Translation: AAQ23714.1.
AJ428063 mRNA. Translation: CAD20985.1.
RefSeqNP_001075362.1. NM_001081893.1.
UniGeneEca.12742.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GVZX-ray1.42A25-261[»]
ProteinModelPortalQ6H321.
SMRQ6H321. Positions 25-261.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9796.ENSECAP00000009529.

Protein family/group databases

MEROPSS01.171.

Proteomic databases

PRIDEQ6H321.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSECAT00000012101; ENSECAP00000009529; ENSECAG00000011643.
GeneID100034023.
KEGGecb:100034023.

Organism-specific databases

CTD100034023.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00750000117405.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidQ6H321.
KOK01325.
OMACATHRDD.
OrthoDBEOG75B84T.
TreeFamTF331065.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ6H321.

Entry information

Entry nameKLK2_HORSE
AccessionPrimary (citable) accession number: Q6H321
Secondary accession number(s): Q8WMN9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 19, 2004
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references