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Q6H321

- KLK2_HORSE

UniProt

Q6H321 - KLK2_HORSE

Protein

Kallikrein-1E2

Gene

KLK1E2

Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (19 Jul 2004)
      Previous versions | rss
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    Functioni

    Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

    Catalytic activityi

    Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Charge relay systemBy similarity
    Active sitei120 – 1201Charge relay systemBy similarity
    Active sitei213 – 2131Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.171.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kallikrein-1E2 (EC:3.4.21.35)
    Alternative name(s):
    Glandular kallikrein
    HPK
    Gene namesi
    Name:KLK1E2
    OrganismiEquus caballus (Horse)
    Taxonomic identifieri9796 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
    ProteomesiUP000002281: Chromosome 10

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717CuratedAdd
    BLAST
    Propeptidei18 – 247Activation peptideCuratedPRO_0000027921
    Chaini25 – 261237Kallikrein-1E2PRO_0000027922Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 173
    Disulfide bondi50 ↔ 66
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi152 ↔ 219
    Disulfide bondi184 ↔ 198
    Disulfide bondi209 ↔ 234

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PRIDEiQ6H321.

    Expressioni

    Tissue specificityi

    Detected in prostate and semen.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi9796.ENSECAP00000009529.

    Structurei

    Secondary structure

    1
    261
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi39 – 446
    Beta strandi47 – 5610
    Beta strandi59 – 624
    Helixi64 – 663
    Beta strandi72 – 765
    Beta strandi88 – 903
    Beta strandi92 – 976
    Helixi103 – 1075
    Beta strandi122 – 1287
    Beta strandi151 – 1588
    Turni162 – 1643
    Beta strandi166 – 17914
    Helixi181 – 1844
    Helixi189 – 1913
    Beta strandi196 – 2005
    Helixi210 – 2123
    Beta strandi216 – 2194
    Beta strandi222 – 2276
    Beta strandi231 – 2333
    Turni237 – 2393
    Beta strandi243 – 2464
    Helixi247 – 2493
    Helixi250 – 26011

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GVZX-ray1.42A25-261[»]
    ProteinModelPortaliQ6H321.
    SMRiQ6H321. Positions 25-261.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ6H321.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 258234Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00750000117405.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiQ6H321.
    KOiK01325.
    OMAiCATHRDD.
    OrthoDBiEOG75B84T.
    TreeFamiTF331065.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q6H321-1 [UniParc]FASTAAdd to Basket

    « Hide

    MWFLVLCLDL SLGETGALPP IQSRIIGGWE CEKHSKPWQV AVYHQGHFQC    50
    GGVLVHPQWV LTAAHCMSDD YQIWLGRHNL SEDEDTAQFH QVSDSFLDPQ 100
    FDLSLLKKKY LRPYDDISHD LMLLRLAQPA RITDAVKILD LPTQEPKLGS 150
    TCYTSGWGLI STFTNRGSGT LQCVELRLQS NEKCARAYPE KMTEFVLCAT 200
    HRDDSGSICL GDSGGALICD GVFQGITSWG YSECADFNDN FVFTKVMPHL 250
    KWIKETIEKN S 261
    Length:261
    Mass (Da):29,392
    Last modified:July 19, 2004 - v1
    Checksum:iDD612F74EAE0CE49
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti82 – 821E → K in CAD20985. (PubMed:12217694)Curated
    Sequence conflicti250 – 2501L → K(PubMed:12217694)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY290704 mRNA. Translation: AAQ23714.1.
    AJ428063 mRNA. Translation: CAD20985.1.
    RefSeqiNP_001075362.1. NM_001081893.1.
    UniGeneiEca.12742.

    Genome annotation databases

    EnsembliENSECAT00000012101; ENSECAP00000009529; ENSECAG00000011643.
    GeneIDi100034023.
    KEGGiecb:100034023.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY290704 mRNA. Translation: AAQ23714.1 .
    AJ428063 mRNA. Translation: CAD20985.1 .
    RefSeqi NP_001075362.1. NM_001081893.1.
    UniGenei Eca.12742.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GVZ X-ray 1.42 A 25-261 [» ]
    ProteinModelPortali Q6H321.
    SMRi Q6H321. Positions 25-261.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9796.ENSECAP00000009529.

    Protein family/group databases

    MEROPSi S01.171.

    Proteomic databases

    PRIDEi Q6H321.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSECAT00000012101 ; ENSECAP00000009529 ; ENSECAG00000011643 .
    GeneIDi 100034023.
    KEGGi ecb:100034023.

    Organism-specific databases

    CTDi 100034023.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00750000117405.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi Q6H321.
    KOi K01325.
    OMAi CATHRDD.
    OrthoDBi EOG75B84T.
    TreeFami TF331065.

    Miscellaneous databases

    EvolutionaryTracei Q6H321.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Taxon-specific evolution of glandular kallikrein genes and identification of a progenitor of prostate-specific antigen."
      Olsson A.Y., Lilja H., Lundwall A.
      Genomics 84:147-156(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Prostate.
    2. "Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: a homologue of human PSA."
      Carvalho A.L., Sanz L., Barettino D., Romero A., Calvete J.J., Romao M.J.
      J. Mol. Biol. 322:325-337(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 62-252, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, X-RAY CRYSTALLOGRAPHY (1.42 ANGSTROMS) OF 25-261.
      Tissue: Prostate.

    Entry informationi

    Entry nameiKLK2_HORSE
    AccessioniPrimary (citable) accession number: Q6H321
    Secondary accession number(s): Q8WMN9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2004
    Last sequence update: July 19, 2004
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3