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Q6H321

- KLK2_HORSE

UniProt

Q6H321 - KLK2_HORSE

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Protein
Kallikrein-1E2
Gene
KLK1E2
Organism
Equus caballus (Horse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay system By similarity
Active sitei120 – 1201Charge relay system By similarity
Active sitei213 – 2131Charge relay system By similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.171.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein-1E2 (EC:3.4.21.35)
Alternative name(s):
Glandular kallikrein
HPK
Gene namesi
Name:KLK1E2
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Chromosome 10

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Inferred
Add
BLAST
Propeptidei18 – 247Activation peptide Inferred
PRO_0000027921
Chaini25 – 261237Kallikrein-1E2
PRO_0000027922Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 173
Disulfide bondi50 ↔ 66
Glycosylationi79 – 791N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi152 ↔ 219
Disulfide bondi184 ↔ 198
Disulfide bondi209 ↔ 234

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ6H321.

Expressioni

Tissue specificityi

Detected in prostate and semen.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9796.ENSECAP00000009529.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi39 – 446
Beta strandi47 – 5610
Beta strandi59 – 624
Helixi64 – 663
Beta strandi72 – 765
Beta strandi88 – 903
Beta strandi92 – 976
Helixi103 – 1075
Beta strandi122 – 1287
Beta strandi151 – 1588
Turni162 – 1643
Beta strandi166 – 17914
Helixi181 – 1844
Helixi189 – 1913
Beta strandi196 – 2005
Helixi210 – 2123
Beta strandi216 – 2194
Beta strandi222 – 2276
Beta strandi231 – 2333
Turni237 – 2393
Beta strandi243 – 2464
Helixi247 – 2493
Helixi250 – 26011

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GVZX-ray1.42A25-261[»]
ProteinModelPortaliQ6H321.
SMRiQ6H321. Positions 25-261.

Miscellaneous databases

EvolutionaryTraceiQ6H321.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 258234Peptidase S1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00750000117405.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ6H321.
KOiK01325.
OMAiCATHRDD.
OrthoDBiEOG75B84T.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6H321-1 [UniParc]FASTAAdd to Basket

« Hide

MWFLVLCLDL SLGETGALPP IQSRIIGGWE CEKHSKPWQV AVYHQGHFQC    50
GGVLVHPQWV LTAAHCMSDD YQIWLGRHNL SEDEDTAQFH QVSDSFLDPQ 100
FDLSLLKKKY LRPYDDISHD LMLLRLAQPA RITDAVKILD LPTQEPKLGS 150
TCYTSGWGLI STFTNRGSGT LQCVELRLQS NEKCARAYPE KMTEFVLCAT 200
HRDDSGSICL GDSGGALICD GVFQGITSWG YSECADFNDN FVFTKVMPHL 250
KWIKETIEKN S 261
Length:261
Mass (Da):29,392
Last modified:July 19, 2004 - v1
Checksum:iDD612F74EAE0CE49
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821E → K in CAD20985. 1 Publication
Sequence conflicti250 – 2501L → K1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY290704 mRNA. Translation: AAQ23714.1.
AJ428063 mRNA. Translation: CAD20985.1.
RefSeqiNP_001075362.1. NM_001081893.1.
UniGeneiEca.12742.

Genome annotation databases

EnsembliENSECAT00000012101; ENSECAP00000009529; ENSECAG00000011643.
GeneIDi100034023.
KEGGiecb:100034023.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY290704 mRNA. Translation: AAQ23714.1 .
AJ428063 mRNA. Translation: CAD20985.1 .
RefSeqi NP_001075362.1. NM_001081893.1.
UniGenei Eca.12742.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GVZ X-ray 1.42 A 25-261 [» ]
ProteinModelPortali Q6H321.
SMRi Q6H321. Positions 25-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9796.ENSECAP00000009529.

Protein family/group databases

MEROPSi S01.171.

Proteomic databases

PRIDEi Q6H321.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSECAT00000012101 ; ENSECAP00000009529 ; ENSECAG00000011643 .
GeneIDi 100034023.
KEGGi ecb:100034023.

Organism-specific databases

CTDi 100034023.

Phylogenomic databases

eggNOGi COG5640.
GeneTreei ENSGT00750000117405.
HOGENOMi HOG000251820.
HOVERGENi HBG013304.
InParanoidi Q6H321.
KOi K01325.
OMAi CATHRDD.
OrthoDBi EOG75B84T.
TreeFami TF331065.

Miscellaneous databases

EvolutionaryTracei Q6H321.

Family and domain databases

InterProi IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view ]
Pfami PF00089. Trypsin. 1 hit.
[Graphical view ]
PRINTSi PR00722. CHYMOTRYPSIN.
SMARTi SM00020. Tryp_SPc. 1 hit.
[Graphical view ]
SUPFAMi SSF50494. SSF50494. 1 hit.
PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Taxon-specific evolution of glandular kallikrein genes and identification of a progenitor of prostate-specific antigen."
    Olsson A.Y., Lilja H., Lundwall A.
    Genomics 84:147-156(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Prostate.
  2. "Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: a homologue of human PSA."
    Carvalho A.L., Sanz L., Barettino D., Romero A., Calvete J.J., Romao M.J.
    J. Mol. Biol. 322:325-337(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 62-252, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, X-RAY CRYSTALLOGRAPHY (1.42 ANGSTROMS) OF 25-261.
    Tissue: Prostate.

Entry informationi

Entry nameiKLK2_HORSE
AccessioniPrimary (citable) accession number: Q6H321
Secondary accession number(s): Q8WMN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 19, 2004
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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