Reviewed, UniProtKB/Swiss-Prot Q6H321 (KLK2_HORSE)

Names and origin

Protein names

Recommended name:
    Kallikrein-1E2
    EC=3.4.21.35
Alternative name(s):
    Glandular kallikrein
    HPK

Gene names

Name:

KLK1E2

Organism

Equus caballus (Horse)

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Subcellular location	Secreted. Ref.2
Tissue specificity	Detected in prostate and semen. Ref.2
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	3D-structure
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

Probable

Propeptide

18 – 24

7

Activation peptide Probable

PRO_0000027921

Chain

25 – 261

237

Kallikrein-1E2

PRO_0000027922

Regions

Domain

25 – 258

234

Peptidase S1

Sites

Active site

65

1

Charge relay system By similarity

Active site

120

1

Charge relay system By similarity

Active site

213

1

Charge relay system By similarity

Amino acid modifications

Glycosylation

79

1

N-linked (GlcNAc...) Potential

Disulfide bond

31 ↔ 173

Disulfide bond

50 ↔ 66

Disulfide bond

152 ↔ 219

Disulfide bond

184 ↔ 198

Disulfide bond

209 ↔ 234

Experimental info

Sequence conflict

82

1

E → K in CAD20985. Ref.2

Sequence conflict

250

1

L → K Ref.2

Secondary structure

1

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261

Helix Strand Turn

Details...

Beta strand

39 – 44

6

Beta strand

47 – 56

10

Beta strand

59 – 62

4

Helix

64 – 66

3

Beta strand

72 – 76

5

Beta strand

88 – 90

3

Beta strand

92 – 97

6

Helix

103 – 107

5

Beta strand

122 – 128

7

Beta strand

151 – 158

8

Turn

162 – 164

3

Beta strand

166 – 179

14

Helix

181 – 184

4

Helix

189 – 191

3

Beta strand

196 – 200

5

Helix

210 – 212

3

Beta strand

216 – 219

4

Beta strand

222 – 227

6

Beta strand

231 – 233

3

Turn

237 – 239

3

Beta strand

243 – 246

4

Helix

247 – 249

3

Helix

250 – 260

11

Sequences

	Sequence		Length	Mass (Da)
	Q6H321-1 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: DD612F74EAE0CE49	FASTA	261	29,392
	10 20 30 40 50 60 MWFLVLCLDL SLGETGALPP IQSRIIGGWE CEKHSKPWQV AVYHQGHFQC GGVLVHPQWV 70 80 90 100 110 120 LTAAHCMSDD YQIWLGRHNL SEDEDTAQFH QVSDSFLDPQ FDLSLLKKKY LRPYDDISHD 130 140 150 160 170 180 LMLLRLAQPA RITDAVKILD LPTQEPKLGS TCYTSGWGLI STFTNRGSGT LQCVELRLQS 190 200 210 220 230 240 NEKCARAYPE KMTEFVLCAT HRDDSGSICL GDSGGALICD GVFQGITSWG YSECADFNDN 250 260 FVFTKVMPHL KWIKETIEKN S

References

[1]

"Taxon-specific evolution of glandular kallikrein genes and identification of a progenitor of prostate-specific antigen." Olsson A.Y., Lilja H., Lundwall A. Genomics 84:147-156(2004) [PubMed: 15203212] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Prostate.

[2]

"Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: a homologue of human PSA." Carvalho A.L., Sanz L., Barettino D., Romero A., Calvete J.J., Romao M.J. J. Mol. Biol. 322:325-337(2002) [PubMed: 12217694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 62-252, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, X-RAY CRYSTALLOGRAPHY (1.42 ANGSTROMS) OF 25-261. Tissue: Prostate.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AY290704 mRNA. Translation: AAQ23714.1.
AJ428063 mRNA. Translation: CAD20985.1.

RefSeq

NP_001075362.1.

UniGene

Eca.12742

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GVZ	X-ray	1.42	A	25-261	[»]

ModBase

Search...

Protein family/group databases

MEROPS

S01.171.

Genome annotation databases

Ensembl

ENSECAG00000011643. Equus caballus. [Contig view]

GeneID

100034023.

KEGG

ecb:100034023.

Phylogenomic databases

HOVERGEN

Q6H321.

OMA

Q6H321. FQHESSA.

Enzyme and pathway databases

BRENDA

3.4.21.35. 1464.

Family and domain databases

InterPro

IPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]

Pfam

PF00089. Trypsin. 1 hit.
[Graphical view]

PRINTS

PR00722. CHYMOTRYPSIN.

SMART

SM00020. Tryp_SPc. 1 hit.
[Graphical view]

PROSITE

PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

KLK2_HORSE

Accession

Primary (citable) accession number: Q6H321
Secondary accession number(s): Q8WMN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2004
Last sequence update:	July 19, 2004
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)