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Protein

Kallikrein-1E2

Gene

KLK1E2

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay systemBy similarity1
Active sitei120Charge relay systemBy similarity1
Active sitei213Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.171.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein-1E2 (EC:3.4.21.35)
Alternative name(s):
Glandular kallikrein
HPK
Gene namesi
Name:KLK1E2
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
Proteomesi
  • UP000002281 Componenti: Chromosome 10

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17CuratedAdd BLAST17
PropeptideiPRO_000002792118 – 24Activation peptideCurated7
ChainiPRO_000002792225 – 261Kallikrein-1E2Add BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 173
Disulfide bondi50 ↔ 66
Glycosylationi79N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi152 ↔ 219
Disulfide bondi184 ↔ 198
Disulfide bondi209 ↔ 234

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ6H321.
PRIDEiQ6H321.

Expressioni

Tissue specificityi

Detected in prostate and semen.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9796.ENSECAP00000009529.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi39 – 44Combined sources6
Beta strandi47 – 56Combined sources10
Beta strandi59 – 62Combined sources4
Helixi64 – 66Combined sources3
Beta strandi72 – 76Combined sources5
Beta strandi88 – 90Combined sources3
Beta strandi92 – 97Combined sources6
Helixi103 – 107Combined sources5
Beta strandi122 – 128Combined sources7
Beta strandi151 – 158Combined sources8
Turni162 – 164Combined sources3
Beta strandi166 – 179Combined sources14
Helixi181 – 184Combined sources4
Helixi189 – 191Combined sources3
Beta strandi196 – 200Combined sources5
Helixi210 – 212Combined sources3
Beta strandi216 – 219Combined sources4
Beta strandi222 – 227Combined sources6
Beta strandi231 – 233Combined sources3
Turni237 – 239Combined sources3
Beta strandi243 – 246Combined sources4
Helixi247 – 249Combined sources3
Helixi250 – 260Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GVZX-ray1.42A25-261[»]
ProteinModelPortaliQ6H321.
SMRiQ6H321.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6H321.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 258Peptidase S1PROSITE-ProRule annotationAdd BLAST234

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ6H321.
KOiK01325.
OMAiCATHRDD.
OrthoDBiEOG091G0DF7.
TreeFamiTF331065.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6H321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWFLVLCLDL SLGETGALPP IQSRIIGGWE CEKHSKPWQV AVYHQGHFQC
60 70 80 90 100
GGVLVHPQWV LTAAHCMSDD YQIWLGRHNL SEDEDTAQFH QVSDSFLDPQ
110 120 130 140 150
FDLSLLKKKY LRPYDDISHD LMLLRLAQPA RITDAVKILD LPTQEPKLGS
160 170 180 190 200
TCYTSGWGLI STFTNRGSGT LQCVELRLQS NEKCARAYPE KMTEFVLCAT
210 220 230 240 250
HRDDSGSICL GDSGGALICD GVFQGITSWG YSECADFNDN FVFTKVMPHL
260
KWIKETIEKN S
Length:261
Mass (Da):29,392
Last modified:July 19, 2004 - v1
Checksum:iDD612F74EAE0CE49
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82E → K in CAD20985 (PubMed:12217694).Curated1
Sequence conflicti250L → K (PubMed:12217694).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY290704 mRNA. Translation: AAQ23714.1.
AJ428063 mRNA. Translation: CAD20985.1.
RefSeqiNP_001075362.1. NM_001081893.1.
UniGeneiEca.12742.

Genome annotation databases

EnsembliENSECAT00000012101; ENSECAP00000009529; ENSECAG00000011643.
GeneIDi100034023.
KEGGiecb:100034023.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY290704 mRNA. Translation: AAQ23714.1.
AJ428063 mRNA. Translation: CAD20985.1.
RefSeqiNP_001075362.1. NM_001081893.1.
UniGeneiEca.12742.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GVZX-ray1.42A25-261[»]
ProteinModelPortaliQ6H321.
SMRiQ6H321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9796.ENSECAP00000009529.

Protein family/group databases

MEROPSiS01.171.

Proteomic databases

PaxDbiQ6H321.
PRIDEiQ6H321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSECAT00000012101; ENSECAP00000009529; ENSECAG00000011643.
GeneIDi100034023.
KEGGiecb:100034023.

Organism-specific databases

CTDi100034023.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ6H321.
KOiK01325.
OMAiCATHRDD.
OrthoDBiEOG091G0DF7.
TreeFamiTF331065.

Miscellaneous databases

EvolutionaryTraceiQ6H321.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKLK2_HORSE
AccessioniPrimary (citable) accession number: Q6H321
Secondary accession number(s): Q8WMN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 19, 2004
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.