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Protein

Kallikrein-1E2

Gene

KLK1E2

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

Catalytic activityi

Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay systemBy similarity
Active sitei120 – 1201Charge relay systemBy similarity
Active sitei213 – 2131Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.171.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein-1E2 (EC:3.4.21.35)
Alternative name(s):
Glandular kallikrein
HPK
Gene namesi
Name:KLK1E2
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
ProteomesiUP000002281: Chromosome 10

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717CuratedAdd
BLAST
Propeptidei18 – 247Activation peptideCuratedPRO_0000027921
Chaini25 – 261237Kallikrein-1E2PRO_0000027922Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 173
Disulfide bondi50 ↔ 66
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi152 ↔ 219
Disulfide bondi184 ↔ 198
Disulfide bondi209 ↔ 234

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiQ6H321.

Expressioni

Tissue specificityi

Detected in prostate and semen.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi9796.ENSECAP00000009529.

Structurei

Secondary structure

1
261
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi39 – 446Combined sources
Beta strandi47 – 5610Combined sources
Beta strandi59 – 624Combined sources
Helixi64 – 663Combined sources
Beta strandi72 – 765Combined sources
Beta strandi88 – 903Combined sources
Beta strandi92 – 976Combined sources
Helixi103 – 1075Combined sources
Beta strandi122 – 1287Combined sources
Beta strandi151 – 1588Combined sources
Turni162 – 1643Combined sources
Beta strandi166 – 17914Combined sources
Helixi181 – 1844Combined sources
Helixi189 – 1913Combined sources
Beta strandi196 – 2005Combined sources
Helixi210 – 2123Combined sources
Beta strandi216 – 2194Combined sources
Beta strandi222 – 2276Combined sources
Beta strandi231 – 2333Combined sources
Turni237 – 2393Combined sources
Beta strandi243 – 2464Combined sources
Helixi247 – 2493Combined sources
Helixi250 – 26011Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GVZX-ray1.42A25-261[»]
ProteinModelPortaliQ6H321.
SMRiQ6H321. Positions 25-261.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ6H321.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 258234Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ6H321.
KOiK01325.
OrthoDBiEOG75B84T.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q6H321-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MWFLVLCLDL SLGETGALPP IQSRIIGGWE CEKHSKPWQV AVYHQGHFQC
60 70 80 90 100
GGVLVHPQWV LTAAHCMSDD YQIWLGRHNL SEDEDTAQFH QVSDSFLDPQ
110 120 130 140 150
FDLSLLKKKY LRPYDDISHD LMLLRLAQPA RITDAVKILD LPTQEPKLGS
160 170 180 190 200
TCYTSGWGLI STFTNRGSGT LQCVELRLQS NEKCARAYPE KMTEFVLCAT
210 220 230 240 250
HRDDSGSICL GDSGGALICD GVFQGITSWG YSECADFNDN FVFTKVMPHL
260
KWIKETIEKN S
Length:261
Mass (Da):29,392
Last modified:July 19, 2004 - v1
Checksum:iDD612F74EAE0CE49
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti82 – 821E → K in CAD20985. (PubMed:12217694)Curated
Sequence conflicti250 – 2501L → K(PubMed:12217694)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY290704 mRNA. Translation: AAQ23714.1.
AJ428063 mRNA. Translation: CAD20985.1.
RefSeqiNP_001075362.1. NM_001081893.1.
UniGeneiEca.12742.

Genome annotation databases

EnsembliENSECAT00000012101; ENSECAP00000009529; ENSECAG00000011643.
GeneIDi100034023.
KEGGiecb:100034023.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY290704 mRNA. Translation: AAQ23714.1.
AJ428063 mRNA. Translation: CAD20985.1.
RefSeqiNP_001075362.1. NM_001081893.1.
UniGeneiEca.12742.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GVZX-ray1.42A25-261[»]
ProteinModelPortaliQ6H321.
SMRiQ6H321. Positions 25-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9796.ENSECAP00000009529.

Protein family/group databases

MEROPSiS01.171.

Proteomic databases

PRIDEiQ6H321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSECAT00000012101; ENSECAP00000009529; ENSECAG00000011643.
GeneIDi100034023.
KEGGiecb:100034023.

Organism-specific databases

CTDi100034023.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ6H321.
KOiK01325.
OrthoDBiEOG75B84T.
TreeFamiTF331065.

Miscellaneous databases

EvolutionaryTraceiQ6H321.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Taxon-specific evolution of glandular kallikrein genes and identification of a progenitor of prostate-specific antigen."
    Olsson A.Y., Lilja H., Lundwall A.
    Genomics 84:147-156(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Prostate.
  2. "Crystal structure of a prostate kallikrein isolated from stallion seminal plasma: a homologue of human PSA."
    Carvalho A.L., Sanz L., Barettino D., Romero A., Calvete J.J., Romao M.J.
    J. Mol. Biol. 322:325-337(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 62-252, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, X-RAY CRYSTALLOGRAPHY (1.42 ANGSTROMS) OF 25-261.
    Tissue: Prostate.

Entry informationi

Entry nameiKLK2_HORSE
AccessioniPrimary (citable) accession number: Q6H321
Secondary accession number(s): Q8WMN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 19, 2004
Last modified: January 7, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.