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Reviewed, UniProtKB/Swiss-Prot Q6H266 (ML2_VISAL)

Last modified June 16, 2009. Version 28. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Beta-galactoside-specific lectin 2
Alternative name(s):
    Beta-galactoside-specific lectin II
    Beta-galactoside-specific lectin III
Cleaved into the following 2 chains:
    1- Recommended name:
            Beta-galactoside-specific lectin 2 chain A
              EC=3.2.2.22
        Alternative name(s):
            ML-2 A
            Beta-galactoside-specific lectin II chain A
            ML-II A
            rRNA N-glycosidase
    2- Recommended name:
            Beta-galactoside-specific lectin 2 chain B
        Alternative name(s):
            ML-2B
            Beta-galactoside-specific lectin II chain B
            ML-II B
OrganismViscum album (European mistletoe)
Taxonomic identifier3972 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Santalales › Santalaceae › Viscum

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Protein attributes

Sequence length567 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

The A chain is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits by removing adenine from position 4,324 of 28S rRNA. The B chain binds to cell receptors and probably facilitates the entry into the cell of the A chain; B chains are also responsible for cell agglutination (lectin activity). Ref.1 UniProtKB P81446

Catalytic activity

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA. UniProtKB P81446

Subunit structure

Disulfide-linked dimer of A and B chains By similarity. UniProtKB P81446

Miscellaneous

Several isoforms exist. Ref.1

Sequence similarities

Belongs to the ribosome-inactivating protein family. Type 2 RIP subfamily.

Contains 2 ricin B-type lectin domains.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Chain34 – 287254Beta-galactoside-specific lectin 2 chain A
PRO_5000092109
Propeptide288 – 30114Connecting peptide By similarity UniProtKB P81446
PRO_0000284728
Chain302 – 567266Beta-galactoside-specific lectin 2 chain B
PRO_5000092110

Regions

Domain309 – 439131Ricin B-type lectin 1
Domain443 – 566124Ricin B-type lectin 2
Region324 – 3263Galactose binding By similarity UniProtKB P81446
Region539 – 5413Galactose binding By similarity UniProtKB P81446

Sites

Active site1981 By similarity UniProtKB Q6ITZ3

Amino acid modifications

Glycosylation1451N-linked (GlcNAc...) Potential
Glycosylation3621N-linked (GlcNAc...) Potential
Glycosylation4401N-linked (GlcNAc...) Potential
Disulfide bond280 ↔ 306Interchain (between A and B chains) By similarity UniProtKB P81446
Disulfide bond322 ↔ 341 By similarity UniProtKB Q6ITZ3
Disulfide bond365 ↔ 382 By similarity UniProtKB P81446
Disulfide bond456 ↔ 469 By similarity UniProtKB P81446
Disulfide bond495 ↔ 512 By similarity UniProtKB P81446

Experimental info

Sequence conflict181V → L in AAR25549. Ref.1
Sequence conflict261A → T in AAR25549. Ref.1
Sequence conflict301S → T in AAR25549. Ref.1
Sequence conflict441K → E in AAR25549. Ref.1
Sequence conflict631I → M in AAR25549. Ref.1
Sequence conflict951W → G in AAR25549. Ref.1
Sequence conflict1271H → D in AAR25549. Ref.1
Sequence conflict1991A → V in AAR25549. Ref.1
Sequence conflict2461V → I in AAR25549. Ref.1
Sequence conflict2891V → A in AAR25551. Ref.1
Sequence conflict3141R → Q in AAR25549. Ref.1
Sequence conflict3311R → H in AAR25549. Ref.1
Sequence conflict364 – 3652SC → RF in AAR25549. Ref.1
Sequence conflict3931Q → L in AAR25549. Ref.1
Sequence conflict4301D → Y in AAR25549. Ref.1
Sequence conflict4421T → I in AAR25549. Ref.1
Sequence conflict4681S → T in AAR25549. Ref.1
Sequence conflict5051S → F in AAR25549. Ref.1
Sequence conflict5561S → N in AAR25549. Ref.1
Sequence conflict5661V → M in AAR25549. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q6H266-1 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 5FC57D56848F741F

FASTA56763,410
        10         20         30         40         50         60 
MNARLASSRA WVWCFLMVGL VCGATAKAES KINYRRISLR VTDKTTGDEY FRFITILRDY 

        70         80         90        100        110        120 
VSIGSFSNDI PLLRQSTIPV SDAQRFVLVE LTNQWGDSIT AAIDVTNLYV VAYQAAGQSY 

       130        140        150        160        170        180 
YLRDAPHGAE RHLFTGTTRS SLPFNGSYAD LERYAGHRDR IPLGREPLLR SVSALHYPGG 

       190        200        210        220        230        240 
STRAQASSII IVIQMISEAA RFNPILWRAR QYINRGVSFL PDVYMLELET SWGRQSTQVQ 

       250        260        270        280        290        300 
QSTDGVFNNP IRLGISTGNF VTLSNVRDVI PSLAIMVFVC RDRSSSSDVH NWPLVIRPVM 

       310        320        330        340        350        360 
VDDVTCTTSE PTVRIVGRNG MCLDVRDSDY RDGSRIQLWP CKSNSDPNQL WTIRRDGTIR 

       370        380        390        400        410        420 
SNGSCLTTYG YTAGSYIMMY DCNRAGWDLT TWQIRGNGII FNPRSKMVIG TPSGSRGTRG 

       430        440        450        460        470        480 
TTFTLQTLDD SLGQSWLASN DTAPREVTIY GFRDLCMETS GGRVWVESCV SSKQNQRWAL 

       490        500        510        520        530        540 
YGDGSIRPKP YQDQCLTSQG DSVRSVINLF SCTAGSPRQR WVFTNKGTIL NLKNGLALDV 

       550        560 
RESNPSLRQI IIFSVSGNPN QMWLPVP

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References

[1]"Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L)."
Kourmanova A.G., Soudarkina O.J., Olsnes S., Kozlov J.V.
Eur. J. Biochem. 271:2350-2360(2004) [PubMed: 15182350] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION.
Tissue: Leaf.

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Cross-references

Sequence databases

AY377894 Genomic DNA. Translation: AAR25549.1.
AY377895 Genomic DNA. Translation: AAR25550.1.
AY377896 mRNA. Translation: AAR25551.1.

3D structure databases

SMRQ6H266. Positions 34-280, 302-567.
ModBaseSearch...

Protein family/group databases

CAZyCBM13. Carbohydrate-Binding Module Family 13.

Enzyme and pathway databases

BRENDA3.2.2.22. 273578.

Family and domain databases

InterProIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
IPR000772. Ricin_B_lectin.
[Graphical view]
Gene3DG3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
PfamPF00652. Ricin_B_lectin. 2 hits.
PF00161. RIP. 1 hit.
[Graphical view]
PRINTSPR00396. SHIGARICIN.
SMARTSM00458. RICIN. 2 hits.
[Graphical view]
PROSITEPS50231. RICIN_B_LECTIN. 2 hits.
PS00275. SHIGA_RICIN. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameML2_VISAL
AccessionPrimary (citable) accession number: Q6H266
Secondary accession number(s): Q6H265, Q6H267
Entry history
Integrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: July 19, 2004
Last modified: June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents