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Q6GZT8

- RIR1_FRG3G

UniProt

Q6GZT8 - RIR1_FRG3G

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Protein

Putative ribonucleoside-diphosphate reductase large subunit

Gene

FV3-038R

Organism
Frog virus 3 (isolate Goorha) (FV-3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601SubstrateBy similarity
Sitei75 – 751Important for hydrogen atom transferBy similarity
Sitei82 – 821Allosteric effector bindingBy similarity
Binding sitei103 – 1031Substrate; via amide nitrogenBy similarity
Sitei112 – 1121Allosteric effector bindingBy similarity
Active sitei256 – 2561Proton acceptorBy similarity
Active sitei258 – 2581Cysteine radical intermediateBy similarity
Active sitei260 – 2601Proton acceptorBy similarity
Sitei273 – 2731Important for hydrogen atom transferBy similarity
Sitei534 – 5341Important for electron transferBy similarity
Sitei535 – 5351Important for electron transferBy similarity
Sitei560 – 5601Interacts with thioredoxin/glutaredoxinBy similarity
Sitei563 – 5631Interacts with thioredoxin/glutaredoxinBy similarity

GO - Molecular functioni

  1. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
ORF Names:FV3-038R
OrganismiFrog virus 3 (isolate Goorha) (FV-3)
Taxonomic identifieri654924 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeRanavirus
Virus hostiAmbystoma (mole salamanders) [TaxID: 8295]
Hyla versicolor (chameleon treefrog) [TaxID: 30343]
Lithobates pipiens (Northern leopard frog) (Rana pipiens) [TaxID: 8404]
Notophthalmus viridescens (Eastern newt) (Triturus viridescens) [TaxID: 8316]
Rana sylvatica (Wood frog) [TaxID: 45438]
ProteomesiUP000008770: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Putative ribonucleoside-diphosphate reductase large subunitPRO_0000410591Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 273Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6GZT8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni74 – 752Substrate bindingBy similarity
Regioni256 – 2605Substrate bindingBy similarity
Regioni400 – 4045Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR000788. RNR_lg_C.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.

Sequencei

Sequence statusi: Complete.

Q6GZT8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSAVSFPIT SQASDMLSKF YGSDLGSLYG TLAEKYSGGK PELAEKLRDY
60 70 80 90 100
ACKGWFGYSS PILTSVTGSG LPISCFLLYV PDTIEGLVDH TSELRWLSVM
110 120 130 140 150
GGGVAGHWDD VRGPSAKSCG TIPFLHTVDA DMSAYWQGKV RRGSYAAYMS
160 170 180 190 200
ISHPDLIEFI TMRTPTGDVN RKNLNLHHGV NITDTFMRCV ERGENWDLVD
210 220 230 240 250
PKSGAVAHTV SARELWERIL ETRFRTGEPY LNFIDTANRG LHPALKRKGL
260 270 280 290 300
KIRGSNLCNE IHLPTAADRT AVCCLSSVNL ERYDEWRETD LVECLVEMLD
310 320 330 340 350
NVIQTFVDEA PLKTPHTARA VRSAAGERSI GLGAMGWARY LQKHRIPFDS
360 370 380 390 400
EEAVRLTGII FRGIKSAAVR ATRALAKERG EAPDLTGYGV RNAHLLAVAP
410 420 430 440 450
NANSALLLGT SPSVEPEIGA AYVHRTRVGS HQAVNPYLKR DLESLGLDTE
460 470 480 490 500
EVWDSIVSNK GSVQHIAALP DSLKKVYKTS FEMDQRVIVR QAAERQRHLC
510 520 530 540 550
QGQSLNLFFP IGADKTNLSD VHRLAWKSGC KGLYYLRTCA GRTGHKIFEA
560
KKNPEKTEEC TACQG
Length:565
Mass (Da):62,363
Last modified:July 19, 2004 - v1
Checksum:i790187621FAFC2E7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY548484 Genomic DNA. Translation: AAT09697.1.
RefSeqiYP_031616.1. NC_005946.1.

Genome annotation databases

GeneIDi2947817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY548484 Genomic DNA. Translation: AAT09697.1 .
RefSeqi YP_031616.1. NC_005946.1.

3D structure databases

ProteinModelPortali Q6GZT8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2947817.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR000788. RNR_lg_C.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomic analyses of frog virus 3, type species of the genus Ranavirus (family Iridoviridae)."
    Tan W.G., Barkman T.J., Gregory Chinchar V., Essani K.
    Virology 323:70-84(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRIR1_FRG3G
AccessioniPrimary (citable) accession number: Q6GZT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: July 19, 2004
Last modified: October 1, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3