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Q6GZT8

- RIR1_FRG3G

UniProt

Q6GZT8 - RIR1_FRG3G

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Protein

Putative ribonucleoside-diphosphate reductase large subunit

Gene
FV3-038R
Organism
Frog virus 3 (isolate Goorha) (FV-3)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601Substrate By similarity
Sitei75 – 751Important for hydrogen atom transfer By similarity
Sitei82 – 821Allosteric effector binding By similarity
Binding sitei103 – 1031Substrate; via amide nitrogen By similarity
Sitei112 – 1121Allosteric effector binding By similarity
Active sitei256 – 2561Proton acceptor By similarity
Active sitei258 – 2581Cysteine radical intermediate By similarity
Active sitei260 – 2601Proton acceptor By similarity
Sitei273 – 2731Important for hydrogen atom transfer By similarity
Sitei534 – 5341Important for electron transfer By similarity
Sitei535 – 5351Important for electron transfer By similarity
Sitei560 – 5601Interacts with thioredoxin/glutaredoxin By similarity
Sitei563 – 5631Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
ORF Names:FV3-038R
OrganismiFrog virus 3 (isolate Goorha) (FV-3)
Taxonomic identifieri654924 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeRanavirus
Virus hostiAmbystoma (mole salamanders) [TaxID: 8295]
Hyla versicolor (chameleon treefrog) [TaxID: 30343]
Lithobates pipiens (Northern leopard frog) (Rana pipiens) [TaxID: 8404]
Notophthalmus viridescens (Eastern newt) (Triturus viridescens) [TaxID: 8316]
Rana sylvatica (Wood frog) [TaxID: 45438]
ProteomesiUP000008770: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 565565Putative ribonucleoside-diphosphate reductase large subunitPRO_0000410591Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 273Redox-active By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ6GZT8.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni74 – 752Substrate binding By similarity
Regioni256 – 2605Substrate binding By similarity
Regioni400 – 4045Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR000788. RNR_lg_C.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.

Sequencei

Sequence statusi: Complete.

Q6GZT8-1 [UniParc]FASTAAdd to Basket

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MTSAVSFPIT SQASDMLSKF YGSDLGSLYG TLAEKYSGGK PELAEKLRDY    50
ACKGWFGYSS PILTSVTGSG LPISCFLLYV PDTIEGLVDH TSELRWLSVM 100
GGGVAGHWDD VRGPSAKSCG TIPFLHTVDA DMSAYWQGKV RRGSYAAYMS 150
ISHPDLIEFI TMRTPTGDVN RKNLNLHHGV NITDTFMRCV ERGENWDLVD 200
PKSGAVAHTV SARELWERIL ETRFRTGEPY LNFIDTANRG LHPALKRKGL 250
KIRGSNLCNE IHLPTAADRT AVCCLSSVNL ERYDEWRETD LVECLVEMLD 300
NVIQTFVDEA PLKTPHTARA VRSAAGERSI GLGAMGWARY LQKHRIPFDS 350
EEAVRLTGII FRGIKSAAVR ATRALAKERG EAPDLTGYGV RNAHLLAVAP 400
NANSALLLGT SPSVEPEIGA AYVHRTRVGS HQAVNPYLKR DLESLGLDTE 450
EVWDSIVSNK GSVQHIAALP DSLKKVYKTS FEMDQRVIVR QAAERQRHLC 500
QGQSLNLFFP IGADKTNLSD VHRLAWKSGC KGLYYLRTCA GRTGHKIFEA 550
KKNPEKTEEC TACQG 565
Length:565
Mass (Da):62,363
Last modified:July 19, 2004 - v1
Checksum:i790187621FAFC2E7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY548484 Genomic DNA. Translation: AAT09697.1.
RefSeqiYP_031616.1. NC_005946.1.

Genome annotation databases

GeneIDi2947817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY548484 Genomic DNA. Translation: AAT09697.1 .
RefSeqi YP_031616.1. NC_005946.1.

3D structure databases

ProteinModelPortali Q6GZT8.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 2947817.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR000788. RNR_lg_C.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
ProtoNeti Search...

Publicationsi

  1. "Comparative genomic analyses of frog virus 3, type species of the genus Ranavirus (family Iridoviridae)."
    Tan W.G., Barkman T.J., Gregory Chinchar V., Essani K.
    Virology 323:70-84(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiRIR1_FRG3G
AccessioniPrimary (citable) accession number: Q6GZT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: July 19, 2004
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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