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Protein

Putative ribonucleoside-diphosphate reductase large subunit

Gene

FV3-038R

Organism
Frog virus 3 (isolate Goorha) (FV-3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction (By similarity).By similarity

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60SubstrateBy similarity1
Sitei75Important for hydrogen atom transferBy similarity1
Sitei82Allosteric effector bindingBy similarity1
Binding sitei103Substrate; via amide nitrogenBy similarity1
Sitei112Allosteric effector bindingBy similarity1
Active sitei256Proton acceptorBy similarity1
Active sitei258Cysteine radical intermediateBy similarity1
Active sitei260Proton acceptorBy similarity1
Sitei273Important for hydrogen atom transferBy similarity1
Sitei534Important for electron transferBy similarity1
Sitei535Important for electron transferBy similarity1
Sitei560Interacts with thioredoxin/glutaredoxinBy similarity1
Sitei563Interacts with thioredoxin/glutaredoxinBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
ORF Names:FV3-038R
OrganismiFrog virus 3 (isolate Goorha) (FV-3)
Taxonomic identifieri654924 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageIridoviridaeRanavirus
Virus hostiAmbystoma (mole salamanders) [TaxID: 8295]
Hyla versicolor (chameleon treefrog) [TaxID: 30343]
Lithobates pipiens (Northern leopard frog) (Rana pipiens) [TaxID: 8404]
Notophthalmus viridescens (Eastern newt) (Triturus viridescens) [TaxID: 8316]
Rana sylvatica (Wood frog) [TaxID: 45438]
Proteomesi
  • UP000008770 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004105911 – 565Putative ribonucleoside-diphosphate reductase large subunitAdd BLAST565

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi75 ↔ 273Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ6GZT8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni74 – 75Substrate bindingBy similarity2
Regioni256 – 260Substrate bindingBy similarity5
Regioni400 – 404Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

KOiK00525.

Family and domain databases

InterProiIPR000788. RNR_lg_C.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 2 hits.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.

Sequencei

Sequence statusi: Complete.

Q6GZT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSAVSFPIT SQASDMLSKF YGSDLGSLYG TLAEKYSGGK PELAEKLRDY
60 70 80 90 100
ACKGWFGYSS PILTSVTGSG LPISCFLLYV PDTIEGLVDH TSELRWLSVM
110 120 130 140 150
GGGVAGHWDD VRGPSAKSCG TIPFLHTVDA DMSAYWQGKV RRGSYAAYMS
160 170 180 190 200
ISHPDLIEFI TMRTPTGDVN RKNLNLHHGV NITDTFMRCV ERGENWDLVD
210 220 230 240 250
PKSGAVAHTV SARELWERIL ETRFRTGEPY LNFIDTANRG LHPALKRKGL
260 270 280 290 300
KIRGSNLCNE IHLPTAADRT AVCCLSSVNL ERYDEWRETD LVECLVEMLD
310 320 330 340 350
NVIQTFVDEA PLKTPHTARA VRSAAGERSI GLGAMGWARY LQKHRIPFDS
360 370 380 390 400
EEAVRLTGII FRGIKSAAVR ATRALAKERG EAPDLTGYGV RNAHLLAVAP
410 420 430 440 450
NANSALLLGT SPSVEPEIGA AYVHRTRVGS HQAVNPYLKR DLESLGLDTE
460 470 480 490 500
EVWDSIVSNK GSVQHIAALP DSLKKVYKTS FEMDQRVIVR QAAERQRHLC
510 520 530 540 550
QGQSLNLFFP IGADKTNLSD VHRLAWKSGC KGLYYLRTCA GRTGHKIFEA
560
KKNPEKTEEC TACQG
Length:565
Mass (Da):62,363
Last modified:July 19, 2004 - v1
Checksum:i790187621FAFC2E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY548484 Genomic DNA. Translation: AAT09697.1.
RefSeqiYP_031616.1. NC_005946.1.

Genome annotation databases

GeneIDi2947817.
KEGGivg:2947817.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY548484 Genomic DNA. Translation: AAT09697.1.
RefSeqiYP_031616.1. NC_005946.1.

3D structure databases

ProteinModelPortaliQ6GZT8.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2947817.
KEGGivg:2947817.

Phylogenomic databases

KOiK00525.

Enzyme and pathway databases

UniPathwayiUPA00326.

Family and domain databases

InterProiIPR000788. RNR_lg_C.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 2 hits.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
ProtoNetiSearch...

Entry informationi

Entry nameiRIR1_FRG3G
AccessioniPrimary (citable) accession number: Q6GZT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 28, 2011
Last sequence update: July 19, 2004
Last modified: September 16, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.