Q6GZQ8 (RIR2_FRG3G) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative ribonucleoside-diphosphate reductase small subunit 067L
EC=1.17.4.1

Gene names

ORF Names:

FV3-067L

Organism

Frog virus 3 (isolate Goorha) (FV-3) [Reference proteome]

Taxonomic identifier

654924 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Iridoviridae › Ranavirus › Frog virus 3

Virus host

Ambystoma (mole salamanders) [TaxID: 8295]
Hyla versicolor (chameleon treefrog) [TaxID: 30343]
Notophthalmus viridescens (Eastern newt) (Triturus viridescens) [TaxID: 8316]
Rana pipiens (Northern leopard frog) [TaxID: 8404]
Rana sylvatica (Wood frog) [TaxID: 45438]

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	Putative ribonucleoside-diphosphate reductase small subunit 067L		PRO_0000410579
Sites
	Active site	169	1	By similarity
	Metal binding	131	1	Iron 1 By similarity
	Metal binding	162	1	Iron 1 By similarity
	Metal binding	162	1	Iron 2 By similarity
	Metal binding	165	1	Iron 1 By similarity
	Metal binding	221	1	Iron 2 By similarity
	Metal binding	255	1	Iron 2 By similarity
	Metal binding	258	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q6GZQ8 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 4E4C594687191C0D	FASTA	387	43,890
	10 20 30 40 50 60 MFPHVTIVEK PGCGNCVTLK RALKEKSVPH TVMPYVPGVP GSYPIATVGG QRVGYREITK 70 80 90 100 110 120 ALAMDMFTTS KCFKPFRYNW AEDIRTKHEK VHWVETEINL RDDVTDWKSG VMTPGEKAFV 130 140 150 160 170 180 SCVLRLFTQL DVEVGALYLD HLTQVFRCNE IRNMLTSFAC REAIHQKAYS LLNDTLGMPD 190 200 210 220 230 240 CEYQAFADYG EMTDKLDFMR EMDCSTLPGL ARSLLRAVLN EGVSLFAAFV MLLTFQRSGK 250 260 270 280 290 300 MKGMGKVVEW SVRDENIHVE GVSKLFETLN SEYPWVVTKD LIELFKEDAE RVAELEEAFV 310 320 330 340 350 360 DLAFQECGPV CGLEPKQVKD YVLYVINLRA ANMGLPKLIE RAPEACPIPW VKWLIDGADL 370 380 TNFFENHVTE YETGGLTGSW SYEPLDI

References

[1]	"Comparative genomic analyses of frog virus 3, type species of the genus Ranavirus (family Iridoviridae)." Tan W.G., Barkman T.J., Gregory Chinchar V., Essani K. Virology 323:70-84(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY548484 Genomic DNA. Translation: AAT09727.1.
RefSeq	YP_031646.1. NC_005946.1.
3D structure databases
ProteinModelPortal	Q6GZQ8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2947767.
Phylogenomic databases
ProtClustDB	CLSP2511500.
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIR2_FRG3G

Accession

Primary (citable) accession number: Q6GZQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 28, 2011
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program