Q6GV23 (RHBL5_TOXGO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rhomboid-like protease 5
EC=3.4.21.105

Alternative name(s):
Microneme protein protease 1
Short name=MPP-1

Gene names

Name:	ROM5
Synonyms:	MPP1

Organism

Toxoplasma gondii

Taxonomic identifier

5811 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Coccidia › Eucoccidiorida › Eimeriorina › Sarcocystidae › Toxoplasma

Protein attributes

Sequence length	841 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine protease involved in intramembrane proteolysis. Cleaves microneme adhesins, such as MIC2. This step is essential for efficient invasion of host cells. Catalyzes intramembrane proteolysis of AMA1. Ref.2 Ref.3
Catalytic activity	Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.
Subcellular location	Membrane; Multi-pass membrane protein. Note: Detected primarily at the posterior surface of intracellular tachyzoites. Detected in patches on the cell surface of extracellular tachyzoites. Concentrated at the posterior end of tachyzoites after induction of microneme secretion prior to invasion of host cells. Ref.2
Developmental stage	Highly expressed in tachyzoites. Detected at low levels in bradyzoites and sporozoites. Ref.2
Sequence similarities	Belongs to the peptidase S54 family.
Sequence caution	The sequence AAT84606.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease Serine protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 841	841	Rhomboid-like protease 5		PRO_0000239078
Regions
	Transmembrane	323 – 343	21	Helical; Potential
	Transmembrane	464 – 484	21	Helical; Potential
	Transmembrane	492 – 512	21	Helical; Potential
	Transmembrane	526 – 546	21	Helical; Potential
	Transmembrane	571 – 590	20	Helical; Potential
	Transmembrane	673 – 693	21	Helical; Potential
	Compositional bias	99 – 150	52	Ser-rich
Sites
	Active site	531	1	Nucleophile By similarity
	Active site	585	1	By similarity
Experimental info
	Mutagenesis	531	1	S → A: Loss of activity. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q6GV23 [UniParc]. Last modified July 19, 2004. Version 1. Checksum: 455856A0DB72BFFC	FASTA	841	92,102
	10 20 30 40 50 60 MSSKGGSSRL GSKDLKKMTS RTERELRDSG RVRGEVERVE KRLRATAKVK EQPPTGDYKR 70 80 90 100 110 120 RALASPGETA APTFLVDSRG IPRKTSSTAP RKATLRPASS SPRLASSSRP TESTLPSSSS 130 140 150 160 170 180 RALQGASSSS SSRPRRLHES ASGRGGSGGS AGELRQEKKR LPELEAAEAA PASCVVELRD 190 200 210 220 230 240 VTARKGRTSP ATPPETAGSS VCGQGSHART AEKLEEGTAS HRDGSRRGSV DAETWATPGD 250 260 270 280 290 300 GSSSHEFESS PQREERMQPQ ETGRRELSSE PRSGDLTKNG GDGGPRRHSC AWRKWREHMI 310 320 330 340 350 360 QSFDITTHPF PPRGDGSPRR GKFLMIFLTS SVLFFVFLQE LVLNVTTFNG RCMSPVLYPS 370 380 390 400 410 420 HDAPESERTP RVISFGYGAC EHNLGVSLFR REETKKDPRG RWTPGPLTER CASGRCASDD 430 440 450 460 470 480 GWPSDLVQRG RAQRSPAAFD SPNPRVFSSL GALDTNKVRN YGEMFRVVWG MFLHGGWMHL 490 500 510 520 530 540 LLNVSCQAQT LWILEPAWGF LRTLSLWIVG GVSGSLLSAV ANPCTVTVGS SGAFYGLLGA 550 560 570 580 590 600 LVPFSIEYWD HIASPAWFLF CVSVLVMVAQ FGNMVGVQGV DNNAHLGGLI GGLLFGFATI 610 620 630 640 650 660 RSVHAFRWQG VAERMASSTL FWWMFPAEKR RSLREDNLQR VAREREERSS GRIPPPKFVW 670 680 690 700 710 720 KFRGHEREWC VRFAAAVGLV TFWSVLWLYL LVPSYYESLS SPPGNFSFLG STGCHCCRVQ 730 740 750 760 770 780 PFPGEEDKLP AFHPVRVNRG LFWCFVSEGV ANLFCGRSSA LNRGADVYGQ TRQFEEALGD 790 800 810 820 830 840 LPSARAGEAP LRIAKEEGES ASVWQRLVKS AKKTYNAVLG NTTTPAAPSA AELAQQTRAG Q

References

[1]	"Host cell invasion by the apicomplexans: the significance of microneme protein proteolysis." Dowse T., Soldati D. Curr. Opin. Microbiol. 7:388-396(2004) [PubMed: 15358257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: RH.
[2]	"A spatially localized rhomboid protease cleaves cell surface adhesins essential for invasion by Toxoplasma." Brossier F., Jewett T.J., Sibley L.D., Urban S. Proc. Natl. Acad. Sci. U.S.A. 102:4146-4151(2005) [PubMed: 15753289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 161-841, FUNCTION, MUTAGENESIS OF SER-531, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[3]	"Intramembrane cleavage of microneme proteins at the surface of the apicomplexan parasite Toxoplasma gondii." Opitz C., Di Cristina M., Reiss M., Ruppert T., Crisanti A., Soldati D. EMBO J. 21:1577-1585(2002) [PubMed: 11927542] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY634626 mRNA. Translation: AAT47708.1. AY587208 mRNA. Translation: AAT84606.1. Different initiation.
3D structure databases
ProteinModelPortal	Q6GV23.
ModBase	Search...
Protein family/group databases
MEROPS	S54.023.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR017092. Pept_S54_Rhomboid-like_Rom4/5. IPR002610. Peptidase_S54_rhomboid. IPR022764. Peptidase_S54_rhomboid_dom. [Graphical view]
PANTHER	PTHR22936. Peptidase_S54_rhomboid. 1 hit.
Pfam	PF01694. Rhomboid. 1 hit. [Graphical view]
PIRSF	PIRSF037023. Rhomboid-like_ROM4_ROM5. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RHBL5_TOXGO

Accession

Primary (citable) accession number: Q6GV23
Secondary accession number(s): Q696L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	July 19, 2004
Last modified:	November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)