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Protein

N-fatty-acyl-amino acid synthase/hydrolase PM20D1

Gene

PM20D1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Bidirectional N-fatty-acyl amino acid synthase/hydrolase that regulates the production of N-fatty-acyl amino acids. These metabolites are endogenous chemical uncouplers of mitochondrial respiration. In an UCP1-independent manner, maybe through interaction with mitochondrial transporters, they promote proton leakage into the mitochondrial matrix. Thereby, this secreted protein may indirectly regulate the bodily dissipation of chemical energy as heat through thermogenic respiration.1 Publication

Catalytic activityi

A N-fatty-acyl-L-amino acid + H2O = a fatty acid + an amino acid.By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi125Zinc 2By similarity1
Active sitei127By similarity1
Metal bindingi157Zinc 1By similarity1
Metal bindingi157Zinc 2By similarity1
Active sitei191Proton acceptorBy similarity1
Metal bindingi192Zinc 1By similarity1
Metal bindingi217Zinc 2By similarity1
Metal bindingi464Zinc 1By similarity1

GO - Molecular functioni

GO - Biological processi

  • amide biosynthetic process Source: UniProtKB
  • cellular amide catabolic process Source: UniProtKB
  • cellular amino acid metabolic process Source: UniProtKB
  • cellular lipid metabolic process Source: UniProtKB
  • negative regulation of neuron death Source: UniProtKB
  • peptide catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Lyase, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM20.011.

Names & Taxonomyi

Protein namesi
Recommended name:
N-fatty-acyl-amino acid synthase/hydrolase PM20D11 Publication (EC:3.5.1.-1 Publication, EC:4.3.-.-1 Publication)
Alternative name(s):
Peptidase M20 domain-containing protein 1Imported
Gene namesi
Name:PM20D1Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:26518. PM20D1.

Subcellular locationi

  • Secreted By similarity

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi148811.
OpenTargetsiENSG00000162877.
PharmGKBiPA162399772.

Polymorphism and mutation databases

BioMutaiPM20D1.
DMDMi317373406.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000032192826 – 502N-fatty-acyl-amino acid synthase/hydrolase PM20D1Add BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi252N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ6GTS8.
PeptideAtlasiQ6GTS8.
PRIDEiQ6GTS8.

PTM databases

iPTMnetiQ6GTS8.
PhosphoSitePlusiQ6GTS8.

Expressioni

Gene expression databases

BgeeiENSG00000162877.
CleanExiHS_PM20D1.
GenevisibleiQ6GTS8. HS.

Organism-specific databases

HPAiHPA017571.

Interactioni

Protein-protein interaction databases

BioGridi127171. 2 interactors.
STRINGi9606.ENSP00000356104.

Structurei

3D structure databases

ProteinModelPortaliQ6GTS8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M20A family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2275. Eukaryota.
COG0624. LUCA.
GeneTreeiENSGT00840000129850.
HOGENOMiHOG000245216.
HOVERGENiHBG059923.
InParanoidiQ6GTS8.
KOiK13049.
OMAiQAYENQV.
OrthoDBiEOG091G0BP2.
PhylomeDBiQ6GTS8.
TreeFamiTF328688.

Family and domain databases

Gene3Di3.30.70.360. 2 hits.
InterProiIPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
SUPFAMiSSF55031. SSF55031. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6GTS8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQRCVCVLA LVAMLLLVFP TVSRSMGPRS GEHQRASRIP SQFSKEERVA
60 70 80 90 100
MKEALKGAIQ IPTVTFSSEK SNTTALAEFG KYIHKVFPTV VSTSFIQHEV
110 120 130 140 150
VEEYSHLFTI QGSDPSLQPY LLMAHFDVVP APEEGWEVPP FSGLERDGII
160 170 180 190 200
YGRGTLDDKN SVMALLQALE LLLIRKYIPR RSFFISLGHD EESSGTGAQR
210 220 230 240 250
ISALLQSRGV QLAFIVDEGG FILDDFIPNF KKPIALIAVS EKGSMNLMLQ
260 270 280 290 300
VNMTSGHSSA PPKETSIGIL AAAVSRLEQT PMPIIFGSGT VVTVLQQLAN
310 320 330 340 350
EFPFPVNIIL SNPWLFEPLI SRFMERNPLT NAIIRTTTAL TIFKAGVKFN
360 370 380 390 400
VIPPVAQATV NFRIHPGQTV QEVLELTKNI VADNRVQFHV LSAFDPLPVS
410 420 430 440 450
PSDDKALGYQ LLRQTVQSVF PEVNITAPVT SIGNTDSRFF TNLTTGIYRF
460 470 480 490 500
YPIYIQPEDF KRIHGVNEKI SVQAYETQVK FIFELIQNAD TDQEPVSHLH

KL
Length:502
Mass (Da):55,741
Last modified:January 11, 2011 - v3
Checksum:i0CC2C45E1A7ADEA8
GO
Isoform 2 (identifier: Q6GTS8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     302-361: FPFPVNIILS...IPPVAQATVN → VYGEKSLNQC...GPRTHEEHCG
     362-502: Missing.

Note: No experimental confirmation available.
Show »
Length:361
Mass (Da):39,544
Checksum:i74340982D6CE39A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84H → R in AAH39170 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03938033H → Y.1 PublicationCorresponds to variant rs11540014dbSNPEnsembl.1
Natural variantiVAR_039381149I → V.2 PublicationsCorresponds to variant rs1891460dbSNPEnsembl.1
Natural variantiVAR_039382153R → W.2 PublicationsCorresponds to variant rs1104899dbSNPEnsembl.1
Natural variantiVAR_039383237I → T.1 PublicationCorresponds to variant rs7518979dbSNPEnsembl.1
Natural variantiVAR_039384258S → C.Corresponds to variant rs11581214dbSNPEnsembl.1
Natural variantiVAR_039385346G → R.1 PublicationCorresponds to variant rs11240573dbSNPEnsembl.1
Natural variantiVAR_039386380I → T.1 PublicationCorresponds to variant rs1361754dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_031826302 – 361FPFPV…QATVN → VYGEKSLNQCNNQDHHGTHH IQSRGQVQCHPPSGPGHSQL PDSPWTDSPRGPRTHEEHCG in isoform 2. 1 PublicationAdd BLAST60
Alternative sequenceiVSP_031827362 – 502Missing in isoform 2. 1 PublicationAdd BLAST141

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057131 mRNA. Translation: BAB71368.1.
AK290786 mRNA. Translation: BAF83475.1.
AC119673 Genomic DNA. No translation available.
BC039170 mRNA. Translation: AAH39170.1.
BC063477 mRNA. Translation: AAH63477.1.
CCDSiCCDS1460.1. [Q6GTS8-1]
RefSeqiNP_689704.4. NM_152491.4. [Q6GTS8-1]
UniGeneiHs.177744.

Genome annotation databases

EnsembliENST00000367136; ENSP00000356104; ENSG00000162877. [Q6GTS8-1]
GeneIDi148811.
KEGGihsa:148811.
UCSCiuc001hdj.4. human. [Q6GTS8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK057131 mRNA. Translation: BAB71368.1.
AK290786 mRNA. Translation: BAF83475.1.
AC119673 Genomic DNA. No translation available.
BC039170 mRNA. Translation: AAH39170.1.
BC063477 mRNA. Translation: AAH63477.1.
CCDSiCCDS1460.1. [Q6GTS8-1]
RefSeqiNP_689704.4. NM_152491.4. [Q6GTS8-1]
UniGeneiHs.177744.

3D structure databases

ProteinModelPortaliQ6GTS8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127171. 2 interactors.
STRINGi9606.ENSP00000356104.

Protein family/group databases

MEROPSiM20.011.

PTM databases

iPTMnetiQ6GTS8.
PhosphoSitePlusiQ6GTS8.

Polymorphism and mutation databases

BioMutaiPM20D1.
DMDMi317373406.

Proteomic databases

PaxDbiQ6GTS8.
PeptideAtlasiQ6GTS8.
PRIDEiQ6GTS8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367136; ENSP00000356104; ENSG00000162877. [Q6GTS8-1]
GeneIDi148811.
KEGGihsa:148811.
UCSCiuc001hdj.4. human. [Q6GTS8-1]

Organism-specific databases

CTDi148811.
DisGeNETi148811.
GeneCardsiPM20D1.
HGNCiHGNC:26518. PM20D1.
HPAiHPA017571.
MIMi617124. gene.
neXtProtiNX_Q6GTS8.
OpenTargetsiENSG00000162877.
PharmGKBiPA162399772.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2275. Eukaryota.
COG0624. LUCA.
GeneTreeiENSGT00840000129850.
HOGENOMiHOG000245216.
HOVERGENiHBG059923.
InParanoidiQ6GTS8.
KOiK13049.
OMAiQAYENQV.
OrthoDBiEOG091G0BP2.
PhylomeDBiQ6GTS8.
TreeFamiTF328688.

Miscellaneous databases

GenomeRNAii148811.
PROiQ6GTS8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000162877.
CleanExiHS_PM20D1.
GenevisibleiQ6GTS8. HS.

Family and domain databases

Gene3Di3.30.70.360. 2 hits.
InterProiIPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
PfamiPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
SUPFAMiSSF55031. SSF55031. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiP20D1_HUMAN
AccessioniPrimary (citable) accession number: Q6GTS8
Secondary accession number(s): Q6P4E3, Q96DM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 11, 2011
Last modified: November 30, 2016
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.