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Protein

Nck-associated protein 5-like

Gene

Nckap5l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates microtubule organization and stabilization. Promotes microtubule growth and bundling formation and stabilizes microtubules by increasing intense acetylation of microtubules. Both tubulin-binding and homodimer formation are required for NCKAP5L-mediated microtubule bundle formation.By similarity

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Nck-associated protein 5-like
Alternative name(s):
Centrosomal protein of 169 kDaBy similarity
Short name:
Cep169By similarity
Gene namesi
Name:Nckap5l
Synonyms:Cep169By similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:3609653. Nckap5l.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002884481 – 1323Nck-associated protein 5-likeAdd BLAST1323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei436Phosphoserine; by CDK1By similarity1
Modified residuei447Phosphoserine; by CDK1By similarity1
Modified residuei466Phosphoserine; by CDK1By similarity1
Modified residuei473Phosphoserine; by CDK1By similarity1
Modified residuei489PhosphoserineBy similarity1
Modified residuei492PhosphoserineBy similarity1
Modified residuei494PhosphoserineBy similarity1
Modified residuei573Phosphoserine; by CDK1By similarity1
Modified residuei654PhosphothreonineBy similarity1
Modified residuei760Phosphoserine; by CDK1By similarity1
Modified residuei1184PhosphoserineBy similarity1

Post-translational modificationi

CDK1/Cyclin B-dependent phosphorylation mediates its dissociation from centrosomes during mitosis.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ6GQX2.
PaxDbiQ6GQX2.
PeptideAtlasiQ6GQX2.
PRIDEiQ6GQX2.

PTM databases

iPTMnetiQ6GQX2.
PhosphoSitePlusiQ6GQX2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000023009.
CleanExiMM_C230021P08RIK.
ExpressionAtlasiQ6GQX2. baseline and differential.
GenevisibleiQ6GQX2. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with CDK5RAP2. Interacts with MAPRE1. Interacts with beta-tubulin.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023747.

Structurei

3D structure databases

ProteinModelPortaliQ6GQX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 135Mediates interaction with CDK5RAP2 and is required for homodimerization and microtubule bundle formationBy similarityAdd BLAST135
Regioni743 – 1136Mediates interaction with beta-tubulin and is required for microtubule bundle formationBy similarityAdd BLAST394

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili22 – 109Sequence analysisAdd BLAST88
Coiled coili942 – 985Sequence analysisAdd BLAST44

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi480 – 483(S/T)X(I/L)P motif 1By similarity4
Motifi808 – 811(S/T)X(I/L)P motif 2By similarity4
Motifi918 – 921(S/T)X(I/L)P motif 3; required for interaction with MAPRE1By similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi112 – 613Pro-richAdd BLAST502
Compositional biasi781 – 871Pro-richAdd BLAST91
Compositional biasi1120 – 1257Pro-richAdd BLAST138

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IHBY. Eukaryota.
ENOG410XSUM. LUCA.
GeneTreeiENSGT00530000063607.
HOGENOMiHOG000113170.
HOVERGENiHBG080689.
InParanoidiQ6GQX2.
OMAiSRIPCRN.
OrthoDBiEOG091G007P.
PhylomeDBiQ6GQX2.
TreeFamiTF331208.

Family and domain databases

InterProiView protein in InterPro
IPR032769. NCKAP5_C.
IPR026163. Nckap5l.
PANTHERiPTHR21740. PTHR21740. 1 hit.
PfamiView protein in Pfam
PF15246. NCKAP5. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GQX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQPAGGTGK LRASAGEDDS MELSTCQELL HRLRELEAEN SALAQANENQ
60 70 80 90 100
RETYERCLDE VANHVVQALL NQKDLREECI KLKKRVFDLE RQNQVLSALL
110 120 130 140 150
QQKLQLTANS LPQIPLTPLQ PPSERPTSPA PNVSEGPATS LPSGLCAGQR
160 170 180 190 200
EVCWEQQLRP GGPGPPATPP PALDALSPFL RKKAQILEVL RALEETDPLL
210 220 230 240 250
LCSPATPWRP TGQGPGSPEP INGEPCGPPQ PEPSPWAPYL LLGPGSLGAL
260 270 280 290 300
LHWERVLGGP GEEEGIRQPW ASSRAPPSAQ GPSSGPHCAP GSSSSSSSDE
310 320 330 340 350
AGDPNEAPSP DTLLGALARK QLNLGQLLGD TETYLQAFLA GATGPLSGDQ
360 370 380 390 400
PGPGKPNSPD PGPPQVSKSK GLPKSAWGAS TPEATRLGFG ATSEGQGPLP
410 420 430 440 450
FLSMFMGAGD APLGSRPGHP HSSSQVKSKL QIGPPSPGDA QGPLLPSPAR
460 470 480 490 500
GLKFLKLPPA SEKVPSPGGP QLSPQLPRSS RIPCRNSGSD GSPSPLLARR
510 520 530 540 550
GLGGGELSPE GAQGLPGSPL PCSAMPDSAQ LRPSQSTVST ALSPGPVVSP
560 570 580 590 600
CFENILDLSR STFRGSPPEP PPSPLQVPTY PQLTLEVPQT PEVLRSPGAP
610 620 630 640 650
SPGLPESCPY SGPQEKSMDR AGSESPHASR RTPGGSSKKP GQGSGRRPGD
660 670 680 690 700
PSHTPLRDRL AALGKLKTGP EGPLGPEKNG VPARSSAEKA RALVRSGECA
710 720 730 740 750
GDVPPSARPL EQPEAKGIFR GAVALGTSSL KQQEPGLTDP GARVYSSHSM
760 770 780 790 800
GARVDLEPIS PRSCLTKVEL AKSRLAGALC PQMPRTPAKV PTSAPSLGKP
810 820 830 840 850
KSPHSSPTKL PSKSPTKVVP RPVVPLGTKE PPKPDKVKGP PWADCGSTVG
860 870 880 890 900
QPTSPVAGPA DPSQGSEGPA PHSAIEEKVM KGIEENVLRL QGQERTPGSE
910 920 930 940 950
AKHRNTSSIA SWFGLKKSKL PALNRRTEAT KNKDGAGGGS PLRKEVKTEA
960 970 980 990 1000
RKLEAESLNI SKLMAKAEDL RRALEEEKAY LSRARPRPGG PATVPSPGLG
1010 1020 1030 1040 1050
QAQGQLAGMY QGADTFMQQL LNRVDGKELP PKSWREPKPE YGDFQPVSTD
1060 1070 1080 1090 1100
PKSPWPACGP RNGLVGPLQG CGKPGKPSSE PGRREEMPSE DSLAEPVSTT
1110 1120 1130 1140 1150
HFTACGSLTR TLDSGIGTFP PPDHSSSGTP SKNLPKTKSL RLDPPPGAPP
1160 1170 1180 1190 1200
ARPPGLTKVP RRAHTLEREV PGIEELLVSG RHPSMPAFPG LLTAPPGHRS
1210 1220 1230 1240 1250
HQTCPDDPCE DPGPPPPVQL AKNWTFPNTR TAGSSSDPFL CPPRQLEGLP
1260 1270 1280 1290 1300
RTPMALPVDR KQSVDPSRTS TPQGPAFGGS RTPSTSDMGE EGRVASGGAP
1310 1320
GLETSESLSD SLYDSLSSCG SQG
Length:1,323
Mass (Da):138,170
Last modified:July 19, 2004 - v1
Checksum:i944DCC62EB77EEF7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti752A → S in BAC33433 (PubMed:16141072).Curated1
Sequence conflicti1100T → A in AAH26468 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC026468 mRNA. Translation: AAH26468.1.
BC072572 mRNA. Translation: AAH72572.1.
AK048721 mRNA. Translation: BAC33433.1.
CCDSiCCDS27820.1.
RefSeqiNP_001001884.1. NM_001001884.1.
XP_006521186.1. XM_006521123.3.
UniGeneiMm.22915.

Genome annotation databases

EnsembliENSMUST00000023747; ENSMUSP00000023747; ENSMUSG00000023009.
GeneIDi380969.
KEGGimmu:380969.
UCSCiuc007xpn.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiNCK5L_MOUSE
AccessioniPrimary (citable) accession number: Q6GQX2
Secondary accession number(s): Q8BX75, Q8R364
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: July 19, 2004
Last modified: June 7, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome