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Reviewed, UniProtKB/Swiss-Prot Q6GQT1 (A2MP_MOUSE)

Last modified February 9, 2010. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alpha-2-macroglobulin-P
Alternative name(s):
    Alpha-2-macroglobulin
Gene names
Name: A2mp
Synonyms: A2m
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length1474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase By similarity. UniProtKB P01023

Subunit structure

Homotetramer; disulfide-linked By similarity. UniProtKB P01023

Subcellular location

Secreted By similarity.

Tissue specificity

Expressed in uterus, mesometrial lympoid aggregate and mammary tissue during pregnancy. Expressed in ovary, testis and kidney. Low level expression in heart. Not expressed in liver. Ref.1

Developmental stage

Expressed in uterus of pregnant females during decidualization from 6 dpc with highest level around 10 dpc declining throughout the rest of the pregnancy. Ref.1

Sequence similarities

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]

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Ontologies

Keywords
   Biological processPregnancy
   Cellular componentSecreted
   DomainBait region
Signal
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMDisulfide bond
Glycoprotein
Thioester bond
Gene Ontology (GO)
   Biological processfemale pregnancy

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular space

Inferred from electronic annotation. Source: InterPro

   Molecular functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 14741442Alpha-2-macroglobulin-P
PRO_0000271402

Regions

Region623 – 752130Bait region By similarity UniProtKB P01023

Amino acid modifications

Glycosylation621N-linked (GlcNAc...) Potential
Glycosylation771N-linked (GlcNAc...) Potential
Glycosylation2531N-linked (GlcNAc...) Potential
Glycosylation4021N-linked (GlcNAc...) Potential
Glycosylation6541N-linked (GlcNAc...) Potential
Glycosylation7741N-linked (GlcNAc...) Potential
Glycosylation8691N-linked (GlcNAc...) Potential
Glycosylation9911N-linked (GlcNAc...) Potential
Glycosylation13661N-linked (GlcNAc...) Potential
Disulfide bond257 ↔ 305 By similarity UniProtKB P01023
Disulfide bond275 ↔ 293 By similarity UniProtKB P01023
Disulfide bond284Interchain (with C-437) By similarity UniProtKB P01023
Disulfide bond437Interchain (with C-284) By similarity UniProtKB P01023
Disulfide bond476 ↔ 569 By similarity UniProtKB P01023
Disulfide bond601 ↔ 771 By similarity UniProtKB P01023
Disulfide bond650 ↔ 697 By similarity UniProtKB P01023
Disulfide bond821 ↔ 849 By similarity UniProtKB P01023
Disulfide bond847 ↔ 883 By similarity UniProtKB P01023
Disulfide bond921 ↔ 1321 By similarity UniProtKB P01023
Disulfide bond1079 ↔ 1127 By similarity UniProtKB P01023
Disulfide bond1352 ↔ 1467 By similarity UniProtKB P01023
Cross-link972 ↔ 975Isoglutamyl cysteine thioester (Cys-Gln) By similarity UniProtKB P01023

Experimental info

Sequence conflict3661R → G in AAO25741. Ref.1
Sequence conflict4521H → R in AAO25741. Ref.1
Sequence conflict659 – 6668NGILYSPV → RNPVLPR in AAO25741. Ref.1
Sequence conflict7991A → P in AAO25741. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q6GQT1-1 [UniParc].

Last modified July 19, 2004. Version 1.
Checksum: 14C6F4F0819EC1ED

FASTA1,474164,327
        10         20         30         40         50         60 
MGKRWLPSLA LLPLPPPLLL LLLLLLPTNA SAPQKPIYMV MVPSLLHAGT PEKGCLLFNH 

        70         80         90        100        110        120 
LNETVTVKVS MESVRGNQSL FTDLVVDKDL FHCASFIVPQ SSSNEVMFLT VQVKGPTHEF 

       130        140        150        160        170        180 
RRRSTVLIKT KESLVFAQTD KPIYKPGQMV RFRVVSLDEN FHPLNELIPL LYIQDSKKNR 

       190        200        210        220        230        240 
IAQWQNFRLE GGLKQLSFPL SSEPTQGSYK VVIRTESGRT VEHPFSVKEF VLPKFEVKVA 

       250        260        270        280        290        300 
VPETITILEE EMNVSVCGIY TYGKPVPGHV TVNICRKYSN PSSCFGEESL AFCEKFSQQL 

       310        320        330        340        350        360 
DGRGCFSQLV KTKSFQLKRQ EYEMQLDVNA KIQEEGTGVE ETGKGLTKIT RTITKLSFVN 

       370        380        390        400        410        420 
VDTHFRQGIP FVGQVLLVDG RGTPIPYEMI FIGADEANQN INTTTDKNGL ARFSINTDDI 

       430        440        450        460        470        480 
MGTSLTVRAK YKDSNVCYGF RWLTEENVEA WHTANAVFSP SRSFVHLESL PYKLRCEQTL 

       490        500        510        520        530        540 
AVQAHYILND EAVLERKELV FYYLMMAKGG IVRAGTHVLP VTQGHKKGHF SILISMETDL 

       550        560        570        580        590        600 
APVARLVLYT ILPNGEVVGD TVKYEIEKCL ANKVDLVFHP NIGLPATRAF LSVMASPQSL 

       610        620        630        640        650        660 
CGLRAVDQSV LLTKPEAELS ASLVYDLLPV KDLTGFPKGV NQQEEDTNGC LKQNDTYING 

       670        680        690        700        710        720 
ILYSPVQNTN EEDMYGFLKD MGLKVFTNLN IRKPKVCERL GVNKIPAAYH LVSQGHMDAF 

       730        740        750        760        770        780 
LESSESPTET TRSYFPETWI WDLVIVDSTG VAEMEVTVPD TITEWKAGAF CLSNDTGLGL 

       790        800        810        820        830        840 
SPVIDFQAFQ PFFVDLTMAY SVIRGEAFTL KATVLNYLQT CIRVGVQLEA SPDFLATPEE 

       850        860        870        880        890        900 
KEQKSHCICM NERHTMSWAV IPKSLGNVNF TVSAEALDSK ELCRNEVPVV PERGKKDTII 

       910        920        930        940        950        960 
KSLLVEPEGL ENEVTFNSLL CPTGAEVSEQ ISLKLPSDVV EESARASVTV LGDILGSAMQ 

       970        980        990       1000       1010       1020 
NTQDLLKMPY GCGEQNMVLF APNIYVLDYL NETEQLTQEI KTKAITYLNT GYQRQLNYKH 

      1030       1040       1050       1060       1070       1080 
RDGSYSTFGD KPGRSHANTW LTAFVLKSFA QARRYIFIDE SHITQALTWL SQQQKDNGCF 

      1090       1100       1110       1120       1130       1140 
RSSGSLLNNA MKGGVEDEVT LSAYITIALL EMSLPVTHPV VRNALFCLDT AWKSARRGAS 

      1150       1160       1170       1180       1190       1200 
GNHVYTKALL AYAFALAGNQ DTKKEILKSL DEEAVKEDNS VHWTRAQKPR VPADLWYQPQ 

      1210       1220       1230       1240       1250       1260 
APSAEVEMTA YVLLAYLTTE LVPTREDLTA AMLIVKWLTK QQNSHGGFSS TQDTVVALHA 

      1270       1280       1290       1300       1310       1320 
LSKYGAATFT RAKKAAHVTI QSSGAFYTKF QVNNDNQLLL QRVTLPTVPG DYTAKVAGEG 

      1330       1340       1350       1360       1370       1380 
CVYLQTSLKY SVLPREKEFP FALVVQTLPG TCEDLKAHTT FQISLNISYI GSRSDSNMAI 

      1390       1400       1410       1420       1430       1440 
ADVKMVSGFI PLKPTVKMLE RSVHVSRTEV SNNHVLIYLD KVSNQMLTLF FMVQQDIPVR 

      1450       1460       1470 
DLKPAIVKVY DYYEKDEFAV AKYSAPCSAG YGNA

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References

« Hide 'large scale' references
[1]"Characterization of a murine alpha 2 macroglobulin gene expressed in reproductive and cardiovascular tissue."
He H., McCartney D.J., Wei Q., Esadeg S., Zhang J., Foster R.A., Hayes M.A., Tayade C., Van Leuven F., Croy B.A.
Biol. Reprod. 72:266-275(2005) [PubMed: 15355875] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: C57BL/6.
Tissue: Placenta.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY185125 mRNA. Translation: AAO25741.1.
BC072642 mRNA. Translation: AAH72642.1.
IPIIPI00454052.
RefSeqNP_783327.2.
UniGeneMm.30151

3D structure databases

HSSPHSSP built from PDB template 1AYO based on UniProtKB Q7SIH1.
SMRQ6GQT1. Positions 132-233, 961-1270, 1338-1474.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ6GQT1.

Protein family/group databases

MEROPSI39.001.

PTM databases

PhosphoSiteQ6GQT1.

Proteomic databases

PRIDEQ6GQT1.

Genome annotation databases

EnsemblENSMUST00000032203; ENSMUSP00000032203; ENSMUSG00000030111; Mus musculus. [Genome view]
GeneID232345.
KEGGmmu:232345.
UCSCuc009doq.1. mouse.

Organism-specific databases

CTD232345.
MGIMGI:2449119. A2m.

Phylogenomic databases

eggNOGroNOG04104.
HOGENOMHBG444284.
HOVERGENQ6GQT1.
InParanoidQ6GQT1.

Gene expression databases

ArrayExpressQ6GQT1.
BgeeQ6GQT1.
CleanExMM_A2M.
GenevestigatorQ6GQT1.

Family and domain databases

InterProIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR019742. MacrogloblnA2_CS.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
Gene3DG3DSA:2.60.40.690. A-macroglobulin_rcpt-bd. 1 hit.
PfamPF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
[Graphical view]
PROSITEPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio381055.
SOURCESearch...

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Entry information

Entry nameA2MP_MOUSE
AccessionPrimary (citable) accession number: Q6GQT1
Secondary accession number(s): Q811S0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: July 19, 2004
Last modified: February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents