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Protein

Dual specificity protein phosphatase CDC14A

Gene

Cdc14a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Dual-specificity phosphatase. Required for centrosome separation and productive cytokinesis during cell division. Dephosphorylates SIRT2 around early anaphase. May dephosphorylate the APC subunit FZR1/CDH1, thereby promoting APC-FZR1 dependent degradation of mitotic cyclins and subsequent exit from mitosis (By similarity).By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei278 – 2781Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

ReactomeiREACT_324252. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase CDC14A (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
CDC14 cell division cycle 14 homolog A
Gene namesi
Name:Cdc14a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:2442676. Cdc14a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 603603Dual specificity protein phosphatase CDC14APRO_0000094877Add
BLAST

Proteomic databases

PRIDEiQ6GQT0.

PTM databases

PhosphoSiteiQ6GQT0.

Expressioni

Gene expression databases

BgeeiQ6GQT0.
GenevisibleiQ6GQT0. MM.

Interactioni

Subunit structurei

Interacts with KIF20A. Interaction is required to localize CDC14 to the midzone of the mitotic spindle (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087950.

Structurei

3D structure databases

ProteinModelPortaliQ6GQT0.
SMRiQ6GQT0. Positions 15-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7 – 162156AAdd
BLAST
Regioni163 – 17614LinkerAdd
BLAST
Regioni177 – 343167BAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi539 – 54911Poly-SerAdd
BLAST
Compositional biasi580 – 5856Poly-Ser

Domaini

Composed of two structurally equivalent A and B domains that adopt a dual specificity protein phosphatase (DSP) fold.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00390000010254.
HOGENOMiHOG000198341.
HOVERGENiHBG050818.
InParanoidiQ6GQT0.
KOiK06639.
OMAiACEFMKD.
OrthoDBiEOG776SPM.
PhylomeDBiQ6GQT0.
TreeFamiTF101053.

Family and domain databases

Gene3Di3.90.190.10. 2 hits.
InterProiIPR029260. DSPn.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR026068. Dual_Pase_CDC14A.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR23339:SF62. PTHR23339:SF62. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF14671. DSPn. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q6GQT0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAESGELIG ACEFMKDRLY FATLRNRPKS TINIHYFSID EELVYENFYA
60 70 80 90 100
DFGPLNLAMV YRYCCKLNKK LKSYSLSRKK IVHYTSFDQR KRANAAFLIG
110 120 130 140 150
AYAVIYLKKT PEEAYRALLS GSNPPYLPFR DASFGNCTYN LTVLDCLQGI
160 170 180 190 200
RKGLQHGFFD FETFDAEEYE HYERVENGDF NWIVPGKFLA FSGPHPKSKI
210 220 230 240 250
ENGYPLHAPE AYFPYFKKNN VTTIVRLNKK IYEAKRFTDA GFEHYDLFFI
260 270 280 290 300
DGSTPSDNIV RRFLNICENT EGAIAVHCKA GLGRTGTLIA CYVMKHYRFT
310 320 330 340 350
HAEIIAWIRI CRPGSIIGPQ QHFLKEKQAS LWVQGDIFRS KLKNRPSSEG
360 370 380 390 400
SITKIISTLD DMSIGANLSK LQSTERIGEN NFEDEDMEIK NNVTQGDKLR
410 420 430 440 450
ALKSQRHPRS SPSCAFRSDD MKGHQRAMAQ TFRLSSSPQP TMSTMKTSKV
460 470 480 490 500
CLSPSVTAKK ISRGSLSSGA NIRSFSINSR LASSLGNLNA GTEEPETKKT
510 520 530 540 550
TSLTKAAFIA SPFTSFLNGS TQTPGRNYPE LNNNQYTRSS NSNSSSSSSG
560 570 580 590 600
LGGNLNSSPV PQSAKPEEHT TILRPSFPGS LSSSSVRFLS RSIPSLQSEY

VHY
Length:603
Mass (Da):67,634
Last modified:December 21, 2004 - v2
Checksum:i496676322A54C078
GO
Isoform 2 (identifier: Q6GQT0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     104-152: Missing.

Note: No experimental confirmation available.
Show »
Length:554
Mass (Da):62,115
Checksum:iD63622E867C09338
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei104 – 15249Missing in isoform 2. 1 PublicationVSP_012324Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC072644 mRNA. Translation: AAH72644.1.
AK036556 mRNA. Translation: BAC29476.1.
CCDSiCCDS38611.1. [Q6GQT0-1]
CCDS51058.1. [Q6GQT0-2]
RefSeqiNP_001074287.1. NM_001080818.2. [Q6GQT0-1]
NP_001167024.1. NM_001173553.1. [Q6GQT0-2]
UniGeneiMm.17647.

Genome annotation databases

EnsembliENSMUST00000090464; ENSMUSP00000087950; ENSMUSG00000033502. [Q6GQT0-1]
ENSMUST00000106491; ENSMUSP00000102100; ENSMUSG00000033502. [Q6GQT0-2]
GeneIDi229776.
KEGGimmu:229776.
UCSCiuc008rca.1. mouse. [Q6GQT0-1]
uc012cwq.1. mouse. [Q6GQT0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC072644 mRNA. Translation: AAH72644.1.
AK036556 mRNA. Translation: BAC29476.1.
CCDSiCCDS38611.1. [Q6GQT0-1]
CCDS51058.1. [Q6GQT0-2]
RefSeqiNP_001074287.1. NM_001080818.2. [Q6GQT0-1]
NP_001167024.1. NM_001173553.1. [Q6GQT0-2]
UniGeneiMm.17647.

3D structure databases

ProteinModelPortaliQ6GQT0.
SMRiQ6GQT0. Positions 15-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000087950.

PTM databases

PhosphoSiteiQ6GQT0.

Proteomic databases

PRIDEiQ6GQT0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090464; ENSMUSP00000087950; ENSMUSG00000033502. [Q6GQT0-1]
ENSMUST00000106491; ENSMUSP00000102100; ENSMUSG00000033502. [Q6GQT0-2]
GeneIDi229776.
KEGGimmu:229776.
UCSCiuc008rca.1. mouse. [Q6GQT0-1]
uc012cwq.1. mouse. [Q6GQT0-2]

Organism-specific databases

CTDi8556.
MGIiMGI:2442676. Cdc14a.

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00390000010254.
HOGENOMiHOG000198341.
HOVERGENiHBG050818.
InParanoidiQ6GQT0.
KOiK06639.
OMAiACEFMKD.
OrthoDBiEOG776SPM.
PhylomeDBiQ6GQT0.
TreeFamiTF101053.

Enzyme and pathway databases

ReactomeiREACT_324252. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.

Miscellaneous databases

NextBioi379667.
PROiQ6GQT0.
SOURCEiSearch...

Gene expression databases

BgeeiQ6GQT0.
GenevisibleiQ6GQT0. MM.

Family and domain databases

Gene3Di3.90.190.10. 2 hits.
InterProiIPR029260. DSPn.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR026068. Dual_Pase_CDC14A.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR23339:SF62. PTHR23339:SF62. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF14671. DSPn. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 72-603 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Bone.

Entry informationi

Entry nameiCC14A_MOUSE
AccessioniPrimary (citable) accession number: Q6GQT0
Secondary accession number(s): Q8BZ66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 23, 2004
Last sequence update: December 21, 2004
Last modified: June 24, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.