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Protein

Ras-related protein Rab-31

Gene

Rab31

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and Mycobacterium (By similarity). Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 198GTPBy similarity
Nucleotide bindingi60 – 645GTPBy similarity
Nucleotide bindingi118 – 1214GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-31
Alternative name(s):
GTP-binding protein Rab0
Gene namesi
Name:Rab31
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi628598. Rab31.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 194194Ras-related protein Rab-31PRO_0000121234Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351PhosphoserineBy similarity
Lipidationi193 – 1931S-geranylgeranyl cysteineBy similarity
Lipidationi194 – 1941S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PRIDEiQ6GQP4.

Expressioni

Tissue specificityi

Detected in brain astrocytes, spleen and intestine (at protein level).2 Publications

Gene expression databases

ExpressionAtlasiQ6GQP4. baseline and differential.
GenevestigatoriQ6GQP4.

Interactioni

Subunit structurei

Interacts (in GDP-bound form) with RIN3 and GAPVD1, which function as guanine exchange factors (GEF). Interacts (in GTP-bound form) with EEA1. Interacts with NGFR. Interacts with EGFR (By similarity). Interacts with OCRL.By similarity1 Publication

Protein-protein interaction databases

IntActiQ6GQP4. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ6GQP4.
SMRiQ6GQP4. Positions 3-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi34 – 429Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ6GQP4.
KOiK07891.
OMAiQTGRRCC.
OrthoDBiEOG77DJ7M.
PhylomeDBiQ6GQP4.
TreeFamiTF331262.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q6GQP4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIRELKVCL LGDTGVGKSS IVCRFVQDHF DHNISPTIGA SFMTKTVPCG
60 70 80 90 100
NELHKFLIWD TAGQERFHSL APMYYRGSAA AVIVYDITKQ DSFHTLKKWV
110 120 130 140 150
KELKEHGPEN IVMAIAGNKC DLSDIREVPL KDAKEYAESI GALVVETSAK
160 170 180 190
NAINIEELFQ GISRQIPPLD PHENGNSGGI KLGNQSLQAG RRCC
Length:194
Mass (Da):21,368
Last modified:April 26, 2005 - v2
Checksum:iD48C9C68E4476205
GO

Sequence cautioni

The sequence AAH72698.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1061H → Y in AAF67746 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF254800 mRNA. Translation: AAF67746.1.
BC072698 mRNA. Translation: AAH72698.1. Different initiation.
RefSeqiNP_659562.2. NM_145094.2.
UniGeneiRn.28921.

Genome annotation databases

GeneIDi246324.
KEGGirno:246324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF254800 mRNA. Translation: AAF67746.1.
BC072698 mRNA. Translation: AAH72698.1. Different initiation.
RefSeqiNP_659562.2. NM_145094.2.
UniGeneiRn.28921.

3D structure databases

ProteinModelPortaliQ6GQP4.
SMRiQ6GQP4. Positions 3-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ6GQP4. 1 interaction.

Proteomic databases

PRIDEiQ6GQP4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi246324.
KEGGirno:246324.

Organism-specific databases

CTDi11031.
RGDi628598. Rab31.

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ6GQP4.
KOiK07891.
OMAiQTGRRCC.
OrthoDBiEOG77DJ7M.
PhylomeDBiQ6GQP4.
TreeFamiTF331262.

Miscellaneous databases

NextBioi623748.
PROiQ6GQP4.

Gene expression databases

ExpressionAtlasiQ6GQP4. baseline and differential.
GenevestigatoriQ6GQP4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Visualization in living cells of formation and transport of vesicles: participation of Rab0, an oligodendrocyte protein."
    Rodriguez-Gabin A.G., Cammer M., Almazan G., Charron M.J., Larocca J.N.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Role of rRAB22b, an oligodendrocyte protein, in regulation of transport of vesicles from trans Golgi to endocytic compartments."
    Rodriguez-Gabin A.G., Cammer M., Almazan G., Charron M., Larocca J.N.
    J. Neurosci. Res. 66:1149-1160(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Rab22B's role in trans-Golgi network membrane dynamics."
    Ng E.L., Wang Y., Tang B.L.
    Biochem. Biophys. Res. Commun. 361:751-757(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Rab22B is expressed in the CNS astroglia lineage and plays a role in epidermal growth factor receptor trafficking in A431 cells."
    Ng E.L., Ng J.J., Liang F., Tang B.L.
    J. Cell. Physiol. 221:716-728(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Interaction of Rab31 and OCRL-1 in oligodendrocytes: its role in transport of mannose 6-phosphate receptors."
    Rodriguez-Gabin A.G., Ortiz E., Demoliner K., Si Q., Almazan G., Larocca J.N.
    J. Neurosci. Res. 88:589-604(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH OCRL.

Entry informationi

Entry nameiRAB31_RAT
AccessioniPrimary (citable) accession number: Q6GQP4
Secondary accession number(s): Q9JK74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: May 27, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.