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Q6GQP4 (RAB31_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ras-related protein Rab-31
Alternative name(s):
GTP-binding protein Rab0
Gene names
Name:Rab31
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length194 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and Mycobacterium By similarity. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Ref.3 Ref.6

Subunit structure

Interacts (in GDP-bound form) with RIN3 and GAPVD1, which function as guanine exchange factors (GEF). Interacts (in GTP-bound form) with EEA1. Interacts with NGFR. Interacts with EGFR By similarity. Interacts with OCRL. Ref.6

Subcellular location

Golgi apparatustrans-Golgi network By similarity. Golgi apparatustrans-Golgi network membrane; Lipid-anchor; Cytoplasmic side By similarity. Early endosome By similarity. Cytoplasmic vesiclephagosome By similarity. Cytoplasmic vesiclephagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Note: Rapidly recruited to phagosomes containing S.aureus or Mycobacterium By similarity.

Tissue specificity

Detected in brain astrocytes, spleen and intestine (at protein level). Ref.4 Ref.5

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Sequence caution

The sequence AAH72698.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Cellular componentCytoplasmic vesicle
Endosome
Golgi apparatus
Membrane
   LigandGTP-binding
Nucleotide-binding
   PTMLipoprotein
Phosphoprotein
Prenylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGolgi to plasma membrane protein transport

Inferred from sequence or structural similarity. Source: UniProtKB

Golgi vesicle transport

Inferred from mutant phenotype Ref.3. Source: RGD

cellular response to insulin stimulus

Inferred from sequence or structural similarity. Source: UniProtKB

phagosome maturation

Inferred from sequence or structural similarity. Source: UniProtKB

receptor internalization

Inferred from sequence or structural similarity. Source: UniProtKB

regulated secretory pathway

Inferred from sequence or structural similarity. Source: UniProtKB

small GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: UniProtKB-SubCell

early endosome

Inferred from sequence or structural similarity. Source: UniProtKB

phagocytic vesicle

Inferred from sequence or structural similarity. Source: UniProtKB

phagocytic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

trans-Golgi network membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionGDP binding

Inferred from sequence or structural similarity. Source: UniProtKB

GTP binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 194194Ras-related protein Rab-31
PRO_0000121234

Regions

Nucleotide binding12 – 198GTP By similarity
Nucleotide binding60 – 645GTP By similarity
Nucleotide binding118 – 1214GTP By similarity
Motif34 – 429Effector region By similarity

Amino acid modifications

Modified residue351Phosphoserine By similarity
Lipidation1931S-geranylgeranyl cysteine By similarity
Lipidation1941S-geranylgeranyl cysteine By similarity

Experimental info

Sequence conflict1061H → Y in AAF67746. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q6GQP4 [UniParc].

Last modified April 26, 2005. Version 2.
Checksum: D48C9C68E4476205

FASTA19421,368
        10         20         30         40         50         60 
MAIRELKVCL LGDTGVGKSS IVCRFVQDHF DHNISPTIGA SFMTKTVPCG NELHKFLIWD 

        70         80         90        100        110        120 
TAGQERFHSL APMYYRGSAA AVIVYDITKQ DSFHTLKKWV KELKEHGPEN IVMAIAGNKC 

       130        140        150        160        170        180 
DLSDIREVPL KDAKEYAESI GALVVETSAK NAINIEELFQ GISRQIPPLD PHENGNSGGI 

       190 
KLGNQSLQAG RRCC 

« Hide

References

« Hide 'large scale' references
[1]"Visualization in living cells of formation and transport of vesicles: participation of Rab0, an oligodendrocyte protein."
Rodriguez-Gabin A.G., Cammer M., Almazan G., Charron M.J., Larocca J.N.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.
[3]"Role of rRAB22b, an oligodendrocyte protein, in regulation of transport of vesicles from trans Golgi to endocytic compartments."
Rodriguez-Gabin A.G., Cammer M., Almazan G., Charron M., Larocca J.N.
J. Neurosci. Res. 66:1149-1160(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Rab22B's role in trans-Golgi network membrane dynamics."
Ng E.L., Wang Y., Tang B.L.
Biochem. Biophys. Res. Commun. 361:751-757(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Rab22B is expressed in the CNS astroglia lineage and plays a role in epidermal growth factor receptor trafficking in A431 cells."
Ng E.L., Ng J.J., Liang F., Tang B.L.
J. Cell. Physiol. 221:716-728(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Interaction of Rab31 and OCRL-1 in oligodendrocytes: its role in transport of mannose 6-phosphate receptors."
Rodriguez-Gabin A.G., Ortiz E., Demoliner K., Si Q., Almazan G., Larocca J.N.
J. Neurosci. Res. 88:589-604(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH OCRL.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF254800 mRNA. Translation: AAF67746.1.
BC072698 mRNA. Translation: AAH72698.1. Different initiation.
RefSeqNP_659562.2. NM_145094.2.
UniGeneRn.28921.

3D structure databases

ProteinModelPortalQ6GQP4.
SMRQ6GQP4. Positions 3-167.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ6GQP4. 1 interaction.

Proteomic databases

PRIDEQ6GQP4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000067974; ENSRNOP00000063486; ENSRNOG00000042189.
GeneID246324.
KEGGrno:246324.

Organism-specific databases

CTD11031.
RGD628598. Rab31.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00640000091139.
HOGENOMHOG000233968.
HOVERGENHBG009351.
KOK07891.
OMAKQIPPLE.
OrthoDBEOG77DJ7M.
PhylomeDBQ6GQP4.
TreeFamTF331262.

Gene expression databases

GenevestigatorQ6GQP4.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
SMARTSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
PROSITEPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio623748.
PROQ6GQP4.

Entry information

Entry nameRAB31_RAT
AccessionPrimary (citable) accession number: Q6GQP4
Secondary accession number(s): Q9JK74
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: April 16, 2014
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families