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Protein

E3 ubiquitin-protein ligase CBL-B-A

Gene

cblb-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.By similarity

Miscellaneous

This protein has one functional calcium-binding site.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei297PhosphotyrosineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi230 – 243PROSITE-ProRule annotationAdd BLAST14
Zinc fingeri384 – 423RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processImmunity, Ubl conjugation pathway
LigandCalcium, Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase CBL-B-A (EC:2.3.2.27By similarity)
Alternative name(s):
RING-type E3 ubiquitin transferase CBL-B-ACurated
SH3-binding protein CBL-B-A
Signal transduction protein CBL-B-A
Gene namesi
Name:cblb-a
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6251733. cblb.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558631 – 918E3 ubiquitin-protein ligase CBL-B-AAdd BLAST918

Proteomic databases

MaxQBiQ6GQL0.
PRIDEiQ6GQL0.

Interactioni

Subunit structurei

Interacts with several SH3 domain-containing proteins and with poly-ubiquitinated proteins.By similarity

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliQ6GQL0.
SMRiQ6GQL0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 354Cbl-PTBPROSITE-ProRule annotationAdd BLAST309

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni46 – 1784HAdd BLAST133
Regioni179 – 251EF-hand-likeAdd BLAST73
Regioni252 – 354SH2-likeAdd BLAST103
Regioni355 – 383LinkerAdd BLAST29

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi487 – 576Pro-richAdd BLAST90
Compositional biasi809 – 873Pro-richAdd BLAST65

Domaini

The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri384 – 423RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOVERGENiHBG005255.
KOiK04707.

Family and domain databases

CDDicd09920. SH2_Cbl-b_TKB. 1 hit.
Gene3Di1.20.930.20. 1 hit.
3.30.40.10. 1 hit.
3.30.505.10. 1 hit.
InterProiView protein in InterPro
IPR024162. Adaptor_Cbl.
IPR014741. Adaptor_Cbl_EF_hand-like.
IPR003153. Adaptor_Cbl_N_hlx.
IPR014742. Adaptor_Cbl_SH2-like.
IPR024159. Cbl_PTB.
IPR011992. EF-hand-dom_pair.
IPR000980. SH2.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PANTHERiPTHR23007. PTHR23007. 1 hit.
PfamiView protein in Pfam
PF02262. Cbl_N. 1 hit.
PF02761. Cbl_N2. 1 hit.
PF02762. Cbl_N3. 1 hit.
SMARTiView protein in SMART
SM00184. RING. 1 hit.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47668. SSF47668. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiView protein in PROSITE
PS51506. CBL_PTB. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q6GQL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGSGSSSS TSSSALSGRL PGSRSANPRK ARILGLFDAI QDAVGPPKQA
60 70 80 90 100
AADRRTVEKT WKLMDKVVRL CQNPKLQLKN SPPYILDILP DTYQHLRLIL
110 120 130 140 150
SKYDDNQKLA QLSENEYFKI YIDSLIKKSK RAMRLFKEGK ERMYEEQSQE
160 170 180 190 200
RRNLTKLSLI FSHMLAEIKA IFPSGQFQGD NFRITKADAA EFWRKFFGER
210 220 230 240 250
TIVPWKIFRQ CLHEVHQISS GLEAMALKST IDLTCNDYIS VFEFDIFTRL
260 270 280 290 300
FQPWGSILRN WNFLAVTHPG YMAFLTYDEV KARLQKYSLK PGSYIFRLSC
310 320 330 340 350
TRLGQWAIGY VTADGNILQT IPHNKPLFQA LIDGSREGFY LYPDGRSYNP
360 370 380 390 400
DLTGLCEPTP HDHIKVTQEQ YELYCEMGST FQLCKICAEN DKDVKIEPCG
410 420 430 440 450
HLMCTSCLTS WQESDGQGCP FCRCEIKGME PIIVDPFDPR DESRCFSFSD
460 470 480 490 500
SLCTPMLDFD DDDLREECLI MNRLAALRKM NERQNSPVTS PGSSPLSQRR
510 520 530 540 550
KTPPEPLQIP HLNLPPVPPR LDLIQKGLAR SPCASPTGSP KSSPCMVRKQ
560 570 580 590 600
DKPLPAPPPL LREPPPPPER PPPIPPDSRT CRHLHHADNV PCRDQSTPHE
610 620 630 640 650
AWCTRDLSGG NPASVCRVTH DGSPKLGVPS SSVLNGRHSR MSTEAGFMRH
660 670 680 690 700
KHHKRRESPL ETLRVYNGLS GNEEYDVPPR LSPPTITIHP IVKCPVLVNS
710 720 730 740 750
VSDKVRNSAE EDDCEYKIPS SHPVSSRLPL HCHSIKHFPR LCENGQCLSN
760 770 780 790 800
GTHNGISEIK KLKPPDQGDV IATSTVPIHF PPARTSAREN HPHGSSLTRT
810 820 830 840 850
PSDYDLLVPH PGEESFDISP PSQPPPPPPA RTCVTEHVMP TALGSRPNSD
860 870 880 890 900
VDLFLPHSDP CPEAPLPPAR RGPGEVKSNR LSQEYDQLPS CPGKGQEKAS
910
NTKGELLLPN QNLIMRPT
Length:918
Mass (Da):102,831
Last modified:July 19, 2004 - v1
Checksum:i5CEB1FDCBCE9D572
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC072732 mRNA. Translation: AAH72732.1.
RefSeqiNP_001085428.1. NM_001091959.1.
UniGeneiXl.65637.

Genome annotation databases

GeneIDi443854.
KEGGixla:443854.

Similar proteinsi

Entry informationi

Entry nameiCBLBA_XENLA
AccessioniPrimary (citable) accession number: Q6GQL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: July 19, 2004
Last modified: August 30, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways